Natural Product Sciences

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Determination of Chiisanoside in Acanthopanax Species by High Performance Liquid Chromatography

  • Published : 2003.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

The content of chiisanoside in the Acanthopanax Species was determined by reversed-phase high performance liquid chromatographic method. Chiisanoside was separated from the other components in the plant extracts using Zorbax 300 SB $C_{18}$ column with gradient elution of acetonitrile. Identification of chiisanoside was carried out by comparison in the LC/MS spectrum of separated peak from extract with that of standard. By HPLC analysis in this experiment, Acanthopanax species could be classified into two groups based upon the content of chiisanoside-one with low concentration of chiisanoside, such as A. senticosus and A. koreanum, and another with high concentration of chiisanoside, such as A. senticosus f. inermis, A. Divaricatus var. albeofructus, and A. chiisanensis.

Keywords

References

  1. Bae, E. A., Yook, C. S., Oh, O. J., Chang, S. Y. Nohara, T., Kim, D. H., Metabolism of chiisanoside from Acanthopanax divaricatus var. albeofructus by human intestinal bacteria and its relation to some biological activities. Bioi. Pharrn. Bull. 24, 582-585 (2001) https://doi.org/10.1248/bpb.24.582
  2. Cho, H. K, Ham, I., Whang, W. K, Constituents and quantitative analysis from the leaves of Acanthopanax divaricatus f. narnbunensis. Yakhak Hoeji 43, 294-299 (1999)
  3. Hahn D. R., Kasai R., Kim J. H., Taniyasu S., and Tanaka O., A new glycosyl ester ofa 3,4-seco-triterpene from Korean medicinal plant, Acanthopanax chiisanensis. Chern. Pharrn. Bull. 32, 1244-1247 (1984) https://doi.org/10.1248/cpb.32.1244
  4. Kang, J. S., Pham, T. L., Cai, X. F.,Kim, H. S., Lee J. J., Kim, Y. H., Quantitative determination of eleutheroside B and E from Acanthopanax species by high performance liquid chromatography. Arch. Pharm. Res., 24,407-411 (2001) https://doi.org/10.1007/BF02975184
  5. Kim, C. J., Hahn, D. R., The biological activity of a new glycoside, chiisanoside from Acanthopanax chiisanensis Nakai leaves. Yakhak Hoeji 24, 123-134 (1980)
  6. Kim, Y. H., Chung, B. S. Sankawa, U., Pimaradiene diterpenes from Acanthopanax koreanurn. J. Nat. Prod., 51, 1080~1083 (1988) https://doi.org/10.1021/np50060a005
  7. Kim, Y. O., Cho, D. H., Chung H. J., Kim, J. H., Chang, S. Y., Yook, C. S., Yang, K. S., Oh, O. J., Effects of lupane-triterpenoids on mitogen-induced proliferation of lymphocytes. Yakhak Hoeji 43, 208-239 (1999)
  8. Nishibe S, Kinoshita H, Takeda H, Okano G., Phenolic compounds from stem bark of Acanthopanax senticosus and their pharmacological effect in chronic swimming stressed rats. Chern. Pharrn. Bull. 38, 1763-1765 (1990) https://doi.org/10.1248/cpb.38.1763
  9. Oh, O. J., Chang, S. Y., Yook, C. S., Yang K. S., Park, S. Y., Nohara, T., Two 3,4-seco-Iupane triterpenes from leaves of Acanthopanax divaricatus var. albeofructus. Chern. Pharrn. Bull. 48, 879-881 (2000) https://doi.org/10.1248/cpb.48.879
  10. Park, S. Y., Chang, S. Y., Yook, C. S., Nohara, T., New 3,4-seco-lupane-type triterpene glycosides from Acanthopanax senticosus forma inermis. J. Nat. Prod. 63, 1630-1633 (2000) https://doi.org/10.1021/np000277c
  11. Park, S. Y. Studies on RAPD analysis and triterpenoidal constituents of Acanthopanax species. PH. D. Dissertation, Kumamoto University (2002)
  12. Perry, L. M., and Metzger, J., Medicinal plants of east and southeast of Asia. MIT Press, Cambridge, MA and London, 1980, pp. 41.
  13. Yook, C. S., Rho, Y. S., Seo, S. H., Leem, J. Y., Han, D. R., Chemical components of Acanthopanax divaricatus and anticancer effect in leaves. Yakhak Hoeji 40, 251-261 (1996)
  14. Yook, C. S., Medicinal herbs of Acanthopanax in Asia. Kyeongwon Media, Seoul, 2001