The Korean Journal of Physiology and Pharmacology

The Korean Society of Pharmacology (대한약리학회)
권호 표지

A Simple and Accurate Method for Determining Antioxidative Activity

  • Bae, Jin-Woo (Department of Pharmacology, Medical College of Konkuk University) ;
  • Lee, Moo-Hong (Department of Pharmacology, Medical College of Konkuk University)
  • Published : 2003.10.21
Download PDF
⟨ Previous Next ⟩

Abstract

Antioxidative activity is an important factor in inhibiting oxidative stress. The usual methods for determining antioxidative activity are time-consuming and cumbersome. They are also indirect processes that use biological material such as brain or liver microsome. This study therefore proposed a new method. Redoxpotential was determined using galvanic cell with or without the addition of various antioxidants or herbal extracts in zinc sulfate solution. The result was compared with the results from the TBA method and the peroxide value from sodium thiosulfate titration. All methods showed significant and dose-dependent enhancement of antioxidative activity by adding ascorbic acid, quercetin, ginseng, or gingko biloba extract. The result of redox potential using galvanic cell showed the smallest standard deviation and took the shortest time among the three methods. Therefore, the antioxidative potential of chemical substances and herbal extracts can be determined simply, directly and accurately in a short period of time using galvanic cell.

Keywords

References

  1. Ahlemeyer B, Bauerbach E, Plath M, Steuber M, Heers C, Tegtrr F, Krieglstein L. Retinoic acid reduces apoptosis and oxidative stress by preservation of SOD protein level. Free Radic Biol Med 30: 1067 1077, 2003 https://doi.org/10.1016/S0891-5849(01)00495-6
  2. Bridi R, Crossetti FP, Steffen VM, Henriques AT. The antioxidant activity of standardized extract of ginkgo biloba (Egb 761) in rats. Phytother Res 15: 449 51, 2001 https://doi.org/10.1002/ptr.814
  3. Choi KM, Lee EJ, Kim YH, Baik SH, Kim YK, Choi DS. Effects of red ginseng on the lipid peroxidation of erythrocyte and antioxidant superoxide dismutase activity in NIDDM patients. Korean J Ginseng Sci 21: 153 159, 1997
  4. Cramer GL, Miller JF, Pendleton RB, Lands WE. Iodometric measurement of lipid hydroperoxides in human plasma. Anal Biochem 193: 204 211, 1991 https://doi.org/10.1016/0003-2697(91)90010-Q
  5. Jeon BS, Kim NM, Park CK, Yang JW, Chang KS. Effect of controlled atmosphere storage on the antioxidative and cytotoxic activities of fresh and red ginseng. Korean J Ginseng Rec 20(3): 261 268, 1996
  6. Imai H, Graham DI, Masayasu H, Macrae IM. Antioxidant ebselen reduces oxidative damage in focal cerebral ischemia. Free Radic Biol Med 34: 56 63, 2003 https://doi.org/10.1016/S0891-5849(02)01180-2
  7. Kim KH, Sung KS, Chang CC. Effects of the antioxidative components to ginsenoside in the liver of 40-week-old mice. J Ginseng Res 24: 162 167, 2000
  8. Latchoumycandane C, Chitra KC, Mathur PP. 2,3,7,8-Tetrachlorodibenzo- p-dioxin (TCDD) induces oxidative stress in the epididymis and epididymal sperm adults rats. Arch Toxicol 77: 280 284, 2003 https://doi.org/10.1007/s00204-003-0439-x
  9. Lee KY, Weintraub ST, Yu BP. Isolation and identification of a phenolic antioxidant from Aloe Barbadensis. Free Radic Biol Med 28: 261 265, 2000 https://doi.org/10.1016/S0891-5849(99)00235-X
  10. Loguercio C, Federico A. Oxidative stress in viral and alcoholic hepatitis. Free Radic Biol Med 34: 1 10, 2003 https://doi.org/10.1016/S0891-5849(02)01167-X
  11. MacDonald-Wicks LK, Garg ML. Vitamin E supplementation in the mitigation of carbon tetrachloride induced oxidative stress in rats (1). J Nutr Biochem 14: 211 218, 2003 https://doi.org/10.1016/S0955-2863(03)00003-2
  12. Moore K, Roberts II LJ. Measurement of lipid peroxidation. Free Rad Res 28: 659 671, 1998 https://doi.org/10.3109/10715769809065821
  13. Onodera K, Omoi NO, Fukui K, Hayasaka T, Shinkai T, Suzuki SK, Urano S. Oxidative damage of rat cerebral cortex and hippocampus and changes in antioxidative defense systems caused by hyperoxia. Free Radic Res 37: 367 372, 2003. https://doi.org/10.1080/1071576031000090019
  14. Yun BS, Lee IK, Kim JP, Chung SH, Shim GS, Yoo ID. Lipid peroxidation inhibitor activity of some constituents isolated from the stem bark of Eucalytptus globules. Arch Pharm Res 23: 147 150, 2000 https://doi.org/10.1007/BF02975503
  15. Sanders RA, Rauscher FM, Watkins III JB. Effects of quercetin on antioxidant defense in streptozotocin-induced diabetic rats. J Biochem Molecular Toxicology 15: 143 149, 2001
  16. Slater TF. Overview of methods used for detecting lipid peroxidation. Methods in Enzymology 115: 283 293, 1984
  17. Vasiljeva CV, Lyubitsky OB, Klebanov GI, Vladimirov YA. Effects of the combined action of flavonoids, ascorbate and tocopherol on peroxidation of phospholipid liposomes induced by Fe2$^+$ ions. Membr Cell Biol 14: 47 56, 2000
  18. Sung KS, Chun C, Kwon YH, Kim KH, Chang CC. Effects of red ginseng component on the antioxidative enzymes activities and lipid peroxidation in the liver of mice. J Ginseng Res 24: 29 34, 2000
  19. Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 95: 351 358, 1979 https://doi.org/10.1016/0003-2697(79)90738-3