자연과학논문집 (The Journal of Natural Sciences)
- 제12권1호
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- Pages.69-79
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- 2002
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- 1225-6196(pISSN)
인광석 가용화 세균의 분리 및 가용화 최적조건
Isolation of Insoluble Phosphate-Solubilizing Bacteria and Optimum Condition for Solubilization
- Kim, Hyoung-Jong (Department of Genetic Engineering, Division of Life Science, Paichai University) ;
- Jeong, Hun-Seob (Gongju City Agricultural Technology Center) ;
- Kim, Jae-Ho (Department of Genetic Engineering, Division of Life Science, Paichai University) ;
- Lee, Jong-Soo (Department of Genetic Engineering, Division of Life Science, Paichai University)
- 발행 : 2002.06.05
초록
PDA-calcium phosphate 평판배지를 이용하여 인산가용화활성을 가진 850종의 세균을 분리한 후 인광석에 대한 인산가용활성이 가장 강한 HS-2 균주를 선발하였다. 선정 균주의 형태학적, 배양학적 및 생리생화학적 특성 등을 조사한 결과 Azotobacter sp. HS-2로 동정되었고, 이 균주을 인광석을 0.1%함유한 Potato dextrose Broth배지 (pH 6.0)에 접종하여
850 strains of phosphate-solubilizing bacteria were isolated from soil of Chung-nam and Daejeon region using 0.5% calcium phosphate containing medium. The HS-2 strain with the highest rock phosphate-solubilizing activity was selected and identified as Azotobacter sp. HS-2 based on the microbiological characteristics. The optimum culture temperature and initial pH of medium for solubilization of rock phosphate were