한방안이비인후피부과학회지 (The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology)
- 제15권2호
- /
- Pages.33-52
- /
- 2002
- /
- 1738-6640(pISSN)
- /
- 2234-4020(eISSN)
牛蒡子추출물 및 분획층이 항알레르기에 미치는 實驗的 硏究
The effects of Arctii fructus extract on the allergenic inflammation reactions.
- 발행 : 20021200
초록
The effects of Arctii fructus extract on allegenic inflammation were investigated using in vivo and in vitro test models. Firstly, the cytotoxicity of Arctii fructus extract was validated using MTT assay. As a result, Arctii fructus extract showed no cytotoxic potential, while SDS, a positive control, revealed strong cytotoxic effect. In LLNA assay, Arctii fructus extract showed no skin allergenicity. Next, the anti-allergic actions of Arctii fructus extract were evaluated using rodent experimental models. The oral, intraperitoneal and intradermal administration of Arctii fructus extract significantly inhibited the compound 48/80-induced vascular permeability documented by Evans blue extravasation. In addition, Arctii fructus extract showed potent inhibitory effect on passive cutaneous anaphylaxis activated by anti-dinitrophenyl (DNP) IgE when orally administered. In an in vitro study, Arctii fructus extract revealed to possess inhibitory potential on the compound 48/80-induced histamine release from rat peritoneal mast cells. Moreover, Arctii fructus extract inhibited the IL-4 and TNF-
키워드