Biotechnology and Bioprocess Engineering:BBE

  • 제7권5호
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  • Pages.281-288
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  • 2002
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Periodic Change in DO Concentration for Efficient Poly-${\beta}$-hydroxy-butyrate Production Using Temperature-inducible Recombinant Escherichia coli with Proteome Analysis

  • Abdul Rahman, Nor Aini (Department of Biochemical Engineering and Science, Kyushu Institute of Technology) ;
  • Shirai, Yoshihito (Department of Biochemical Engineering and Science, Kyushu Institute of Technology) ;
  • Shimizu, Kazuyuki (Department of Biochemical Engineering and Science, Kyushu Institute of Technology) ;
  • Hassan, Mohd Ali (Department of Biotechnology, University Putra Malaysia)
  • 발행 : 2002.09.01
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초록

Recombinant Escherichia coli strain harboring the ${\lambda}$pR-pL promotor and heterologus poly-${\beta}$-hydroxybutyrate (PHB) biosynthesis genes was used to investigate the effect of culture conditions on the efficient PHB production. The expression of phb genes was induced by a temperature upshift from $33^{\circ}C\;to\;38^{\circ}C$. The protein expression levels were measured by using two-dimensional electrophoresis, and the enzyme activities were also measured to understand the effect of culture temperature, carbon sources, and the dissolved oxygen (DO) concentration on the metabolic regulations. AcetylCoA is an important branch point for PHB production. The decrease in DO concentration lowers the citrate synthase activity, thus limit the flux toward the TCA cycle, and increase the flux for PHB production. Since NADPH is required for PHB production, the PHB production does not continue leading the overproduction of acetate and lac-tate. Based on these observations, a new operation was considered where DO concentration was changed periodically, and it was verified its usefulness for the efficient PHB production by experiments.

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