대한두경부종양학회지 (Korean Journal of Head & Neck Oncology)

  • 제18권1호
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  • Pages.11-17
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  • 2002
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  • 1229-5183(pISSN)
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  • 2586-2553(eISSN)
대한두경부종양학회 (The Korean Society for Head and Neck Oncology)
권호 표지

두경부 편평세포암에서 HMGI(Y), p53, Ki-67의 발현과 임상인자와의 상관 관계

Correlation of Clinical Factors with HMGI(Y), p53 and Ki-67 Expression in Squamous Cell Carcinomas of the Head and Neck

  • 노영수 (한림대학교 의과대학 이비인후-두경부외과학교실) ;
  • 박준영 (한림대학교 의과대학 이비인후-두경부외과학교실) ;
  • 박일석 (한림대학교 의과대학 이비인후-두경부외과학교실) ;
  • 임영창 (한림대학교 의과대학 이비인후-두경부외과학교실) ;
  • 문성호 (한림대학교 의과대학 이비인후-두경부외과학교실) ;
  • 김성동 (한림대학교 의과대학 이비인후-두경부외과학교실) ;
  • 황준식 (한림대학교 의과대학 이비인후-두경부외과학교실) ;
  • 김덕환 (한림대학교 의과대학 해부병리학과교실)
  • Rho Young-Soo (Department of Otorhinolayngology-Head and Neck Surgery, Hallym University College of Medicine) ;
  • Park Jun-Young (Department of Otorhinolayngology-Head and Neck Surgery, Hallym University College of Medicine) ;
  • Park Il-Seok (Department of Otorhinolayngology-Head and Neck Surgery, Hallym University College of Medicine) ;
  • Lim Young-Chang (Department of Otorhinolayngology-Head and Neck Surgery, Hallym University College of Medicine) ;
  • Moon Sung-Ho (Department of Otorhinolayngology-Head and Neck Surgery, Hallym University College of Medicine) ;
  • Kim Sung-Dong (Department of Otorhinolayngology-Head and Neck Surgery, Hallym University College of Medicine) ;
  • Hwang Joon-Sik (Department of Otorhinolayngology-Head and Neck Surgery, Hallym University College of Medicine) ;
  • Kim Duk-Hwan (Department of Pathology, Hallym University College of Medicine)
  • 발행 : 2002.05.01
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초록

Objectives: Expression of HMGI(Y), a nucleoprotein that binds to A/T rich sequences in the minor groove of the DNA helix, is observed in neoplastically transformed cells but not in normal cells. We have analyzed HMGI(Y), p53 expression and Ki-67 labelling index in squamous cell carcinomas of the head and neck, and evaluated its clinicopathologic significance. Materials and Methods: 40 cases of squamous cell carcinoma of the head and neck were entered on the study of immunohistochemical stains for HMGI(Y), p53 and Ki-67. We analyzed the relationship between HMGI(Y), p53, Ki-67 expression and age, sex, primary tumor site, stage, survival rate, recurrence. Results: HMGI(Y) expression evidenced by immunohistochemical staining was observed in 35 of 40 (87.5%) squamous cell carcinoma of the head and neck. But no significant correlation was observed between HMGI(Y) expression and other clinical factors such as primary site, tumor stage, differenciation, cervical lymph node, metastasis, recurrence and immunohistochemical status of p53. The Ki-67 labelling index was significantly correlated with recurrence and HMGI(Y) expression (p<0.05). Conclusion: This results suggest the Ki-67 is a good prognostic factor and the HMGI(Y) expression plays some roles in carcinogenesis and cellular proliferation of squamous cell carcinoma of the head and neck. HMGI(Y) gene can be used as a cancer marker, the correlation between the gene expression and the prognosis of the cancer patient should be proved in the future studies.

키워드

참고문헌

  1. Jones AS, Roland NJ, Caslin AW,Cooke TG : A comparison of cellular proliferation markers in squamous cell carcinoma ofthe head and neck. J Laryngol Otol. 1994 ; 108 : 859-864
  2. Giancotti V, Berlingieri MT, Di Fiore PP, Fusco A, Vecchio G, Crane-Robionson C : Changes in nuclear proteins on transformation of rat epithelial thyroid cells by a murine sarcoma retrovirus. Cancer Res. 1985 ; 45 : 6051-6057
  3. Fedele M, Bandiera A, Chiappetta G, et al : Human colorectal carcinomas express high levels ofhigh mobility group HMG1 (Y) proteins. Cancer Res. 1996 ; 56 : 1896-1901
  4. Bandiera A, Bonifacio D, Manfiloletti G, et al : Expression of HMG1 (Y) proteins in squamous intraepithlial and invasive lesions ofthe uterine cervix. Cancer Res. 1998 ; 58 : 426-431
  5. Chiappeta G, Bandiera A, Berlingieri MT, et al : The expression ofthe high mobility group HMGI (Y) proteins correlates with the malignant phenotype of human thyroid neoplasias. Oncogene. 1995 ; 10 : 1307-1314
  6. Levine AJ, Momand J, Finlay CA : The p53 tumor supressor gene. Nature. 1991 ; 351 : 453-456 https://doi.org/10.1038/351453a0
  7. Watling DL, Gown AM, Coltrera MD : Overexpression of p53 in head and neck cancer. Head-Neck. 1992 ; 14 : 437-444 https://doi.org/10.1002/hed.2880140603
  8. Kamel OW, Franklin WA, Ringus JC, Meyer JS : Thymindine labeling index and Ki-67 growth fraction in lesions ofthe breast. Amer J Pathol. 1989 ; 134 : 107-113
  9. Zhou X, Chada K : HMG1family proteins: architectural transcription factors in mammalian development and cancer. Keio J Med. 1998 ; 47 (2) : 73-77 https://doi.org/10.2302/kjm.47.73
  10. Friedmann M, Holth LT, Zoghbi HY, Reeves R : Organization, inducible expression, and chromosomal localization ofthe human HMGI-I (Y) nonhistone protein gene. Nucleic Acids Res. 1993 : 21 : 4259-4267 https://doi.org/10.1093/nar/21.18.4259
  11. Schoenmakers EF, Wanschura S, Mols R, Bullerdiek J, Van de Yen WJ : Recurrent rearrangements in the high mobility group protein gene, HMGI-C, in benign mesenchymal tumors. Nature Genet. 1995 ;10 : 436-444 https://doi.org/10.1038/ng0895-436
  12. Zhou X, Benson K, Ashar HR, Chada K : Mutation responsible for the mouse pygmy phenotype in the developmentally regulated factor HMGI-C. Nature. 1995 ; 376 : 771-774 https://doi.org/10.1038/376771a0
  13. Chiappetta G, Tallini MC, De Biasio G, Manfio1ettiF, MartinezTello FJ, Pentimalli F : Detection of high mobility group I HMGI (Y) proteins in the diagnosis of thyroid tumors : HMGI (Y) expression represents a potential diagnostic indicator of carcinoma. Cancer Res. 1998 ; 58 : 4193-4198
  14. VogelsteinB, Fearon ER, Hamilton SR, et al : Genetic alterationsduring colorectal tumor development. N Engl J Med. 1998 ; 319 : 525-532 https://doi.org/10.1056/NEJM198809013190901
  15. Giancotti V, Buratti E, Perissin L, et al : Analysis of the HMGI nuclear protein in mouse neoplastic cells induced by different procedures. Exp Cell Res. 1989 ; 184 : 538-545 https://doi.org/10.1016/0014-4827(89)90352-2
  16. Tamimi Y, van der Poe1HG, Karthaus HFM, Debruyne PM, Schalken JA : A retrospective study of high mobility group protein I (Y) as progression markerfor prostate cancer determined by in situ hybridization. Br J Cancer. 1996 ; 74 : 573-578 https://doi.org/10.1038/bjc.1996.403
  17. Rajeswari MR, Singh D, Jain A, Ray R : Elevated levels of high-mobility-group chromosomal proteins, HMGAI, in murine skin carcinoma. Cancer Lett. 2001 ; 173 : 93-99 https://doi.org/10.1016/S0304-3835(01)00688-7
  18. Giancotti V, Pani B, Andrea PD, et al : Elevated levels of specific class of nuclear phosphoproteins in cells transformed with v-ras and v-mos oncogenes and by contransfection with c-myc audpolyoma middle T genes. Embo J. 1987 ; 6 : 1981-1987
  19. Kim DH, Park YS, Park CA, Son KC, Nam ES, Shin HS : Expression ofthe HMGI (Y) gene in human colorectal cancer. Int J Cancer. 1999; 84 : 376-380 https://doi.org/10.1002/(SICI)1097-0215(19990820)84:4<376::AID-IJC8>3.0.CO;2-N
  20. Gerdes J, Li L, Schlueter C, et al : Immunohistochemical and molecular biologic characterization ofthe cell proliferation-associated nuclear antigen that is defined by monoclonal antibody Ki-67 study. Am J Pathol. 1991 ; 138 : 867-873
  21. Gasparini G, Boracchi P, Verderio P : Cell kinetics in human breast cancer .: Comparison between the prognostic value ofthe cytoflurometric S-phase fraction and that ofthe antibodies to Ki67 and PCNA antigens detected by imunocytochemistry. Int J Cancer. 1994 ; 57 : 822-829
  22. Hopf NJ, Bremm J, Bohl J, Pemeczky A : Image analysis of proliferating cells in tumors of the human nervous system : an immunohistological study with the monoclonal antibody Ki-67. Neurosurg. 1994 ; 35 : 917-923 https://doi.org/10.1227/00006123-199411000-00017
  23. Porschen R, Lohe B, Heg1esKJ, Borchard F : Assessment ofcell proliferation in colorectal carcinoma using the monoclonal antibody Ki-67. Cancer. 1989 ; 63 : 1607-1611 https://doi.org/10.1002/1097-0142(19890415)63:8<1607::AID-CNCR2820630827>3.0.CO;2-1