대한수의학회지 (Korean Journal of Veterinary Research)

  • 제42권1호
  • /
  • Pages.1-6
  • /
  • 2002
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  • 2466-1384(pISSN)
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  • 2466-1392(eISSN)
대한수의학회 (The Korean Society of Veterinary Science)
권호 표지

사람, 토끼 및 개 유래 말초혈액 림프구의 미소핵을 이용한 방사선 피폭의 생물학적 선량측정

Induction of micronuclei in human, rabbit and dog lymphocytes irradiated in vitro with gamma radiation

  • Ryu, Si-yun (College of Veterinary Medicine, Chungnam National University) ;
  • Kang, Bit-na (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Ho-jun (College of Veterinary Medicine, Chungnam National University) ;
  • Kim, Tae-hwan (Korea Cancer Center Hospital) ;
  • Jeong, Kyu-sik (College of Veterinary Medicine, Kyungpook National University) ;
  • Kim, Se-ra (College of Veterinary Medicine, Chonnam National University) ;
  • Lee, Hae-june (College of Veterinary Medicine, Chonnam National University) ;
  • Kim, Sung-ho (College of Veterinary Medicine, Chonnam National University) ;
  • An, Mi-Young (College of Veterinary Medicine, Texas A&M University)
  • 심사 : 2002.02.27
  • 발행 : 2002.03.25
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초록

The frequencies of gamma-ray-induced micronuclei (MN) in cytokinesis-blocked (CB) lymphocytes at several doses were measured in three donors of three species (human, rabbit, dog). Measurements performed after irradiation showed a dose-related increases in MN frequency in each of the donors studied. When analysed by linear-quadratic model the line of best fit was : human : $y=0.1184D+0.01867D^2+0.01$, rabbit : $y=0.0387D+0.00528D^2+0.01$ (y = number of MN/CB cells and D = irradiation dose in Gy). The relative sensitivity of rabbit lymphocytes compared with human lymphocytes was estimated by best fitting linear-quadratic model based on the radiation-induced MN data over the range from 0 Gy to 4 Gy. In the case of MN frequency with 0.2, the relative sensitivities of rabbit lymphocytes was 0.39. These data indicate that the induction of MN in rabbit CB cells following irradiation was much less sensitive to the MN induction effects of gamma-irradiation than those from human. The MN assay with dog lymphocytes was very difficult and time-consumed because the dog PHA-stimulated lymphocytes yielded cultures with very low level of CB cells formation in the condition of this experiment. Our in vitro radiobiological study confirmed that the cytogenetic response obtained in blood from rabbit can be utilized for application in environmental studies.

키워드

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