DOI QR코드

DOI QR Code

Structural Alterations in the Gill of the Red Sea Bream, Pagrus major, Exposed to the Harmful Dinoflagellate Cochlodinium polykrikoides

  • Kim Chang Sook (Harmful Algal Blooms Research Division National Fisheries Research and Development Institue) ;
  • Jee Bo-Young (Pathology Division, National Fisheries Research and Development Institute) ;
  • Bae Heon Meen (Harmful Algal Blooms Research Division National Fisheries Research and Development Institue)
  • Published : 2002.03.01

Abstract

The effects of Coehlodinium polykrikoides on the gill of red sea bream, Pagrus major, were examined to clarify the ichthyotoxic mechanisms of this plankton species. The gill of fish exposed to dense blooms over 3,000 cells/mL for 24 h showed severe epithelial separation: a severe edema was found in the secondary lamellar epithelium and interlamellar regions of primary filament. In addition, lipid peroxidation of gill tissue in Coehlodinium-exposed fish was about 2.5 times higher than that of control. The composition of glycoproteins in the gill mucus of Coehlodinium-exposed fish was also changed. These results suggest that the loss of structural integrity of cell membranes in fish gill may be deeply involved in fish death by C. polykrikoides.

Keywords

References

  1. Buege, J.A. and S.D. Aust. 1978. Microsomal lipid peroxida tion. Methods Enzymol., 52, 302-310 https://doi.org/10.1016/S0076-6879(78)52032-6
  2. Endo, M., T. Sakai and A, Kuroki. 1985. Histological and his-tochemical changes in the gills of the yellowtail Seriola quinqueradiata exposed to the raphidphycean flagellate Chattonella marina. Mar. Biol., 87, 193-197 https://doi.org/10.1007/BF00539428
  3. Girotti, A.W. 1990. Photodynamic lipid peroxidation in bio-logical systems. Photochem. Photobiol., 51, 497-509 https://doi.org/10.1111/j.1751-1097.1990.tb01744.x
  4. Hart, P.K. and G.B. Oglesby. 1979. Toxin induced changes in the gill epithelium of rainbow trout (Salmo eairdnen). Scanning Electron Micros., 197, 355-361
  5. Kim, C.S., H.M. Bae, S.J. Yun, Y.C. Cho and H.G. Kim. 2000a. Ichthyotoxicity of a harmful dinoflagellate Coch10-dinium polykrikoides: Aspect of hematological responses of fish exposed to algal blooms. Kor. J. Fish. Sci. Tech., 3, 111-117
  6. Kim, C.S., S.G. Lee, C.K. Lee, H.G. Kim and J. Jung. 1999. Reactive oxygen species as causative agents in the ichthyotoxicity of the red tide dinoflagellate Cochlodinium polykrikoides. J. Plankton Res., 21, 2105-2115 https://doi.org/10.1093/plankt/21.11.2105
  7. Kim, C.S., S.G. Lee and H.G. Kim. 2000b. Biochemical responses of fish exposed to a harmful dinoflagellate Cochlodinium polykrikoides. J. Exp. Mar. Biol. Eco., 254, 131-141 https://doi.org/10.1016/S0022-0981(00)00263-X
  8. Kim, C.S., S.G. Lee, H.K. Kim and J.S. Lee. 2001. Screening for toxic compounds in the red tide dinoflagellate Coch-lodinium polykrikoides: Is it toxic Plankton ? . Algae, 16, 457-462
  9. Kim, H.G., S.G. Lee and K.H. An. 1997. Recent red tides in Korean coastal waters. Nat. Fish. Res. Dev. Agency., pp. 237-239 (in Korean)
  10. Kobayashi, H., Y. Takahashi and T. Itami. 1989. Changes in the electrophoretic pattern of mucous solution extracted from gills of the yellowtail Seriola quinqueradiata affected by the red tide. Nippon Suisan Gakkaishi, 55, 577 https://doi.org/10.2331/suisan.55.577
  11. Lee, J.S. 1996. Bioactive components from red tide plankton, Cochlodinium polykrikoides. J.Kor. Fish. Soc., 29, 165-173 (in Korean)
  12. Onoue, Y., K. Nozawa, K. Kumanda, K. Takeda and T. Aramaki. 1985. Toxicity of Cochlodinium type 78 Yat-sushiro occurring in Yatsushiro Sea. Bull. Jap. Soc. Sci. Fish., 51, 147 https://doi.org/10.2331/suisan.51.147
  13. Onoue, Y. and K. Nozawa. 1989a. Zinc-bound PSP toxins separated from Cochlodinium red tide. In Mycotoxin and Phycotoxins 88', S. Natori, K. Hashimoto and Y. Ueno, ed. Elsevier, Amsterdam, PP. 359-66
  14. Onoue, Y. and K. Nozawa. 1989b. Separation of toxins from harmful red tides occurring along the coast of Kago-shima prefecture, In Red Tides: Biology, Environmental Science, and Toxicology, T. Okaichi, D.M. Anderson and T. Nemoto, eds. Elsevier, New York, PP. 371-374
  15. Toyoshima, T., H.S. Ozaki, M. Shimada, T. Okaichi and T.H. Murakami. 1985. Ultrastructural alterations on chloride cells of the yellowtail Seriola quinqueradiata, following exposure to the red tide species Chattonella antiqua. Mar. Biol 88, 101-108 https://doi.org/10.1007/BF00393048

Cited by

  1. Cochlodinium Red Tide Effects on the Respiration of Abalone, Haliotis discus hannai Ino vol.22, pp.3, 2007, https://doi.org/10.4490/algae.2007.22.3.241
  2. Survey of survival rate and histological alterations of gills and hepatopancreas of the Litopenaeus vannamei juveniles caused by exposure of Margalefidinium / Cochlodinium polykrikoides isolated from vol.97, pp.None, 2002, https://doi.org/10.1016/j.hal.2020.101856