Fisheries and Aquatic Sciences

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지
DOI QR코드

DOI QR Code

Apoptosis in CHSE-214 Chinook Salmon Embryo Cells Infected with Hirame Rhabdovirus (HIRRV)

  • Sung Han-Gi (Department of Biology, Pusan National University) ;
  • Kim Yeong-Jin (Department of Fish Pathology, Yosu National University) ;
  • Jung Sung-Ju (Department of Fish Pathology, Yosu National University) ;
  • Choi Won-Chul (Department of Biology, Pusan National University) ;
  • Jung Tae-Sung (Lab of Fish & Shellfish Disease, College of Veterinary Gyeongsang National University) ;
  • Choi Tae-Jin (Department of Microbiology, Pukyong National University) ;
  • Oh Myung-Joo (Department of Fish Pathology, Yosu National University)
  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

In this study, we investigated the mechanism of cell death in rhabdovirus-infected cells, chinook salmon embryonic cell line (CHSE-2l4) infected with hirame rhabdovirus (HIRRV). Studies using light microscopy, fluorescence microscopy, TUNEL method, electron microscopy, and agarose gel electrophoresis revealed changes in the cell morphology and DNA fragmentation indicative of apoptosis in early infection. It was observed that HIRRV induced apoptosis as well as necrosis in infected cells.

Keywords

References

  1. Bjorklund, H.V., T.R. Johansson and A. Rinne. 1997. Rhab-dovirus-induced apoptosis in a fish cell line is inhibited by a human endogenous acid cysteine proteinase inhibitor. J. Virol., 71, 5658-5662
  2. Hong, J.R., T.L. Lin, Y.L. Hsu and J.L. Wu. 1998. Induction of apoptosis and secondary necrosis by infectious Pan-creatic necrosis virus in fish embryonic cells, J. Fish Dis., 2, 463-467
  3. Imajoh, M. and S. Suzuki. 1999. Apoptosis induced by amarine birnavirus in Established cell lines from fish. J. Fish Pathol., 34, 73-79 https://doi.org/10.3147/jsfp.34.73
  4. Janicke, R.U., M.L. Sprengart, M.R. Wati and A.G. Porter. 1998. Caspase-3 is required for DNA fragmentation and morphological changes associated with apoptosis. J. Biol. Chem., 273, 9357-9360 https://doi.org/10.1074/jbc.273.16.9357
  5. Kerr, J.F.R. and B.V. Harmon. 1991. Definition and incidence of apoptosis: an historical perspective, In: Apoptosis: The molecular basis of cell death L.D. Tomoei & F.O. Cope, ed., Cold Spring Harbor Laboratory Press, Cold Spring Harbor. NY, PP. 5-29
  6. Kim, Y.J., W.C. Choi, H.R. Kim, S.J. Jung, T.S. Jung, J.H. Kim, I.K. Yeo and M.J. Oh. 2000. Relationship between viral propagation and apoptosis after marine birnavirus (MABV) infection. J. Fish. Sci. Tech., 3, 49-51
  7. Oh, M.J. and T.J. Choi. 1999. A new rhabdovirus (HRV-like) isolated in Korea from cultured Japanese flounder Paralichthys olivaceus. J. Fish Pathol., 11, 129-136
  8. Peitsch, M.C., C. Muller and J. Tschopp. 1993. DNA frag-mentation during apoptosis is caused by frequent Single-strand cuts. Nucleic Acids Res., 21, 4206-4209 https://doi.org/10.1093/nar/21.18.4206
  9. Rojo, M.C. and M.E. Gonzalez. 1998. In situ detection of apoptotic cell by TUNEL in the gill epithelium of the developing brown trout (Salmo trutta). J. Anat., 193, 391-398 https://doi.org/10.1046/j.1469-7580.1998.19330391.x
  10. Wyllie, A.H. 1980. Glucocorticoid-induced thymocyte apoptosis is associated with endoeeneous endonuclease activation. Nature, 284, 555-556 https://doi.org/10.1038/284555a0