Journal of Periodontal and Implant Science

Korean Academy of Periodontology (대한치주과학회)
권호 표지

Antimicorbial effect of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogen and effect on human gingival fibroblast cellular activity

옥수수 불검화 추출물(Zea Mays L.)과 후박(Magnoliae cortex) 추출물 혼합물의 치주질환원인균에 대한 항균작용 및 치은섬유아세포 활성도에 미치는 영향

  • Kim, Tae-Il (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Choi, Eun-Jeong (Department of Dentistry, School of Medicine, Eulji University) ;
  • Chung, Chong-Pyoung (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Han, Soo-Boo (Department of Periodontology, College of Dentistry, Seoul National University) ;
  • Ku, Young (Department of Periodontology, College of Dentistry, Seoul National University)
  • 김태일 (서울대학교 치과대학 치주과학교실) ;
  • 최은정 (을지의과대학교 치과학교실) ;
  • 정종평 (서울대학교 치과대학 치주과학교실) ;
  • 한수부 (서울대학교 치과대학 치주과학교실) ;
  • 구영 (서울대학교 치과대학 치주과학교실)
  • Published : 2002.03.30
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Abstract

Zea Mays L. has been known to be effective for improving tissue health and Magnoliae cortex to have effective antibacterial and antimicrobial activity against pathogenic microbes. The purpose of this study was to examine the antimicrobial effects of Zea Mays L. and Magnoliae cortex extract mixtures on periodontal pathogens(Prevotella intermedia, Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis, Streptococcus mutans )and to examine the effects on human gingival fibroblast cellular activity. Zea Mays L. and Magnoliae cortex extracts and their mixtures were prepared with various mixing ratios (0.5:1, 1:1, 1.5:1, 2:1). These extracts were loaded to periodontal pathogen cultured petri dish for antimicrobial test and also loaded to cultured human gingival fibroblast for cellular activity test. Each test was repeated 3 times and data were analyzed by one-way ANOVA with 95% confidence level. Mixture of these two extracts showed greater amount of inhibition area on periodontal pathogen and more improved gingival fibroblast activity as Zea Mays L. ratio reduced. So, mixture ratio 0.5:1 (Zea Mays L. : Magnoliae cortex) group showed statistical significance in antimicrobial activity and cellular activity among various mixtures(p < 0.05). In conclusion, 0.5:1 (Zea Mays L. : Magnoliae cortex) mixture possessed best gingival fibroblast cellular activity and antimicrobial activity toward periodontal pathogens.

Keywords

References

  1. Loe H.,Schiott CR.: The effect of mouth rinses and topical application of Chlorhexidine on the development of dental plaque and gingivitis in man. J Periodont Res 5:79-83,1970 https://doi.org/10.1111/j.1600-0765.1970.tb00696.x
  2. Smith RN,Andersen RN, Kolenbrander PE: Inhibition of intergeneric coaggregation among oral bacteria by cetylpyridium chloride, chlorhexidine digluconate and octenidine dihydrochloride. J Periodont Res 26:422-428,1991 https://doi.org/10.1111/j.1600-0765.1991.tb01732.x
  3. Helgeland K,Heyden G,Rolla G : Effect of chlorhexidine on animal cells in vitro. Scan J Dent Res 79:209-215, 1971
  4. Pucher JJ,Daniel JC: The effects of chlorhexidine digluconate on human fibroblasts in vitro. J Periodont 62: 526-532,1993
  5. Thiers H, Jouanneteau, Zwingelstein: The maize germ oil insaponifiable. Its therapeutic indications. Presse Medi- cale 66:1293,1958
  6. Chaput A: Insadol and parodontolyses. L'Intormation Dentaire 23: 2148,1964
  7. Tecucianu J: Double blind clinical trial of titrated extract of the unsapo- nifiable fractions of Zea Mays L. on gingival inflammation. Inf. Den 57:27, 1975
  8. Son S: Influence of standard extract of the unsaponifiable fraction of Zea Mays L. on periodontal disease. Quintessence international 8:1,1982
  9. Scopp IW, Kumar W,Kasseuny DY, Ozick J: Corn-germ oil extracts in the treatment of periodontal disease. Minerva Stomat. 20:88-93, 1971 253
  10. Fourel J, Siau T, Barka A: Clinical trials of the ussaponifiable part of Maize seed oil in periodontic practice. L'Information Dentaire 8:749-753,1967
  11. Colombel J, Parente C: Trial of the unsaponifiable fraction of Zea Mays L. on dryness of the mouth induced by psychotropic drugs. Progres Odonto Stomatologiques 12:31-33, 1974
  12. Namba T,Tsunezuka M,Bae K, Hattori M.: Studies on dental caries prevention by traditional Chinese medicine (part I). Screening of crude drug for anti- bacterial action against streptococcus mutans. Shoyakugaku Zasshi 35, 1981
  13. Bae K, Oh H: Synergistic effect of lysozyme on bactericidal activity of Magnolol and honokiol against a cariogenic bacterium, Streptococcus mutans OMZ 176. Arch Pharmacies 13: 117,1990 https://doi.org/10.1007/BF02857847
  14. Chang BS, Son SH, Chung CP, Bae KH: The effects of honokiol and magnolol on the antimicrobial, bacterial collagenase activity, cytotoxicity and cytokine production. J Korean Acad Periodont 23: 145-158,1993
  15. 이승렬,정종평,최상묵,배기환: 천연물 추출물의 치주병인균에 대한 항균효과 및 세포독성에 관한 연구. 대한치주과학회지 22:515-526.1992
  16. 이용무,구영,배기환,정종평: 후박 및 대조 추출혼합물이 골조직 재생에 미치 는 영향. 대한치주과학회지 27(1): 165-177, 1997
  17. 신승윤, 이용무, 구영, 배기환, 정종평: 후박 및 홍화종자 추출혼합물이 치주 인대세포 및 골아세포의 활성도 및 백서 의 두개골재생에 미치는 영향. 대한치주과학회지 28: 545-557,1998
  18. 정종평, 구영, 배기환: 후박 및 은행엽 추출물의 항균, 항염 및 세포활성도에 미치는 영향. 대한치주과학회지 25: 478-486. 1995
  19. 정종평,구영,배기환 : 은행엽 추출물이 치은섬유아세포에 미치는 생물학적 영향. 대한치주과학회지 25:469-477,1995
  20. 김태일,염혜리,류인철,배기환,정종평: 후박 및 은행엽 추출물을 함유한 치약의 임상 및 미생물학적 효과에 관한 연구. 대한치주과학회지 26:542-556,1996.
  21. Shin SY, Lee YM, Ku Y, Ryu IC, Han SB, Choi SM, Bae KH, Chung CP: Effects of Magnoliae cortex and Zizyphi fructus extract mixtures on the progression of experimental periodontitis in Beagle dogs. J Korean Acad Periodont 29: 289-296,1999
  22. 이승렬,정종평,최상묵,배기환: 천연물 추출물의 치주병인균에 대한 항균효과 및 세포독성에 관한 연구. 대한치주과학회지 22: 515-526, 1992
  23. Goldschmidt P, Cogen R, Taubman S: Cytopathologic effects of chlorhexidine on human cells. J Periodontol 48:212- 215,1977 https://doi.org/10.1902/jop.1977.48.4.212
  24. 김종관,채중규,조규성,문익상,최성호: Zea Mays L. 불검화 정량추출물의 치주염 치료 효과에 대한 임상적 연구. 대한 치주과학회지 21(2): 225-234,1991
  25. 민원기, 이만섭: Ascorbic acid와 Zea Mays L. 불검화 정량추출물이 치주염 치유에 미치는 영향에 관한 실험적 연구. 대한치주과학회지 18(2):6-23,1988