The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY (한국해양학회지:바다)

The Korean Society of Oceanography (한국해양학회)
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Relative Influence of Sediments, Food and Dissolved Sources on Ag Bioaccumulation in the Amphipod Leptocheirus plumulosus

오염된 퇴적물로부터 해양저서 단각류 Leptocheirus plumulosus의 은(Ag)축적에서 흡수경로의 상대적 기여도 평가

  • Yoo, Hoon (Department of Oceanography Chonnam National University) ;
  • Lee, In-Tae (Department of Oceanography Chonnam National University) ;
  • Lee, Byeong-Gweon (Department of Oceanography Chonnam National University)
  • 유훈 (전남대학교 해양학과) ;
  • 이인태 (전남대학교 해양학과) ;
  • 이병권 (전남대학교 해양학과)
  • Published : 2002.05.31
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Abstract

A amphipod, Leptocheirus plumulosus was exposed to Ag contaminated sediments to evaluate relative importance of various uptake routes (sediment, porewater, supplementary food) for Ag bioaccumulation in sediment-dwelling marine invertebrates. Additionally, influence of AVS (acid-volatile sulfide) on the partitioning of Ag to porewater and on the Ag bioavailability was determined to evaluate the utility of AVS criteria for the management of metal contaminated sediment. The experimental sediments were spiked with 4 levels of Ag (0.1-3.3 ${\mu}$mol Ag/g) and AVS concentrations were manipulated to 40 or <0.5 ${\mu}$mol/g, then equilibrated for >2 months to allow pore water/particulate distributions similar to nature. A L. plumulosus was incubated in the contaminated sediments with overlying water for 35d. During the exposure, the amphipods was fed with supplementary food ($TetraMin^{(R)}$) with or without Ag contamination. Following exposure, tissue Ag in L. plumulosus was strongly correlated with the weak acid extractable Ag in sediments ($r^{2}$=0.87, p<0.001). The ratio of AVS to Ag-SEM (Ag extracted simulaneouls with AVS) had a strong influence on porewater Ag concentration, consistent with previous studies. However, Ag bioaccumulation in L. plumulosus was not influenced by AVS concentrations. The amphipods fed Ag contaminated food took up ${\sim}$ 1.8 X Ag accumulated by the amphipods fed uncontaminated supplementary diet. The result suggests that the benthic invertebrates exposed to metal contaminated sediments would accumulate metals largely via ingestion of contaminated sediments and food, with minor contribution from dissolved sources of porewater and overlying water.

해양퇴적물 오염평가에 널리 쓰이는 단각류 Leptocheirus plumulosus를 이용하여 오염된 퇴적물에서 Ag을 축적하는데 있어서 각 매질(퇴적물, 공극수, 먹이)의 상대적 기여도를 조사하였다. 또한 퇴적물 중 중금속의 생물 이용도와 독성을 조절하는 인자로 잘 알려진 황화물(AVS)이 공극수 중 용존 Ag 농도와 생물내 흡수에 미치는 영향을 평가하였다. 연안 퇴적물을 임의로 4개의 농도구배(0.1${\sim}$3.3 ${\mu}$mol Ag/g)로 오염시킨 후, AVS농도를 40 또는 <0.5 ${\mu}$mol/g로 조절하여 실험생물을 35일 동안 노출시켰다. 실험생물이 배양되는 동안 Ag으로 오염된 것과 오염되지 않은 $TetraMin^{(R)}$을 보조 먹이로 공급하여 생물 체내 Ag축적 농도 차이를 비교하였다. 그 결과, L. plumulosus의 체내 Ag농도는 퇴적물 내 Ag의 농도와 양의 상관관계를 갖고 증가하였다($r^{2}$=0.87, p<0.001). 퇴적물 중 AVS의 함량은 공극수 중 용존 Ag의 농도를 조절하는 중요한 인자로 확인되었으나, AVS 농도는 L. plumulosus의 Ag축적에 거의 영향을 주지 않았다. Ag으로 오염된 먹이가 제공된 L. plumulosus는 오염되지 않은 먹이가 제공된 L. plumulosus보다 최고 1.8배 정도 높게 Ag을 체내에 축적하였다. 즉, Ag으로 오염된 퇴적물에서 L. plumulosus의 Ag축적은 공극수나 해수 중의 용존 Ag의 흡수보다는 퇴적물이나 먹이의 섭식을 통한 흡수가 주요 노출경로라는것을 시사한다.

Keywords

References

  1. Toxicol. Chem v.18 Predicting toxicty of sediments spiked with silver Berry, W.J;M.G. Cantwell;P.A. Edward;J.R. Serbst;D.J. Hansen https://doi.org/10.1897/1551-5028(1999)018<0040:PTOSSW>2.3.CO;2
  2. The Science of the Total Environment v.125 Bioaccumlation, toxicity and physico-chemical speciation of silver in bivalve mollusks: ecotoxicological and health consequences Berthet, B;J.C. Amiard;C. Amiard triquet;M. Martoja;A.Y. Jeantet https://doi.org/10.1016/0048-9697(92)90385-6
  3. Research Report. U.S.Environmental Protection Agency, Narragansett, RI Vertical and seasonal variability of acid volatile sulfides in marine sediments Boothman, W.S;A. Helmstetter
  4. Mar. Ecol. Prog. Ser v.124 Use of the euryhaline bivalve Potamocorbula amrensis as a biosentinel species to assess trace metal contamination in San Francisco Brown, C.L;S.N. Luoma https://doi.org/10.3354/meps124129
  5. Water Res v.29 Sediment pore water collection methods: A review Bufflap, W.E;H.E. Allen https://doi.org/10.1016/0043-1354(94)E0105-F
  6. CBP/TRS 89/93. U.S. Environmental Protection Agency, Newport, OR Development of a chronic sediment toxicity test for marine benthic amphipods Dewitt, T.H;M.S. Redmond;J.E. Sewall;R.C. Swartz
  7. Environ. Sci. Technol v.26 Acid volatile sulfide predicts the acute toxicity of cadmium and nickel in sediments Di Toro, D.M;J.D. Mahony;D.J. Hansen;K.J. Scott;A.R. Carson;G.T. Ankley https://doi.org/10.1021/es00025a009
  8. Environ. Toxicol. Chem v.17 Toxicity and fate of silver in the environment Gorsuch, J.W;S.J. Klaine https://doi.org/10.1897/1551-5028(1998)017<0537:TAFOSI>2.3.CO;2
  9. Sci. Technol v.34 Geochemical influences on assimilation of sediment-bound metals in clams and mussels Griscom, S.B;N.S. Fisher;S.N. Luoma https://doi.org/10.1021/es981309+
  10. Environ. Toxicol. Chem v.15 Chronic effect of cadmium in sediment on colonization by benthic marine organisms: an evaluation of the role of interstitial cadmium and acid-volatile sulfide in biological availability Hansen, D.J;J.D. Mahony;W.J. Berry;S.J. Benyi;J.M. Corbin;S.D. Prarr;D.M. Di Toro;M.B. Abel https://doi.org/10.1897/1551-5028(1996)015<2126:CEOCIS>2.3.CO;2
  11. Environ. Toxical. Chem v.17 Bioaccumulation of silver from laboratory-spiked sediments in the oligochaete(Lumbriculus variegatus) Hirsch, M.P https://doi.org/10.1897/1551-5028(1998)017<0605:BOSFLS>2.3.CO;2
  12. Environ. Toxicol. Chem v.15 Toxicity, silver accumulation and metallothionein induction in freshwater rain bow trout during exposure to different silver salts Hogstrand, C;F. Galvez;C.M. Wood https://doi.org/10.1897/1551-5028(1996)015<1102:TSAAMI>2.3.CO;2
  13. Environ. Toxicol. Chem v.20-3 Sublethal effects of silver in zooplankton: Importance of exposure pathways and implications for toxicity testing Hook, S.E;N.S. Fisher
  14. Environ. Toxicol. Chem v.13 Bioaccumulation of metals by Hyalella azteca exposed to contaminated sediments from the upper Clark Fork River, Montana Ingersoll, C.G;W.G. Brumbaugh;F.J. Dwyer;N.E. Kemble https://doi.org/10.1897/1552-8618(1994)13[2013:BAOMBH]2.0.CO;2
  15. Science v.287 Influence of dietary uptake and reactive sulfides on metal bioavailability from aquatic sediments Lee, B.G;S.B. Griscom;J.S. Lee;H.J. Choi;C.H. Koh;S.N. Luoma;N.S. Fisher https://doi.org/10.1126/science.287.5451.282
  16. Environ. Sci. Technol v.34 Influence of acid volatile sulfiders and metal concentrations on the bioavailability to marine invertebrates in contaminated sediments Lee, B.G;J.S. Lee;H.J. Choi;S.N. Luoma;C.H. Koh https://doi.org/10.1021/es001033h
  17. Mar. Ecol. Prog. Ser v.216 Influence of reactive sulfide(AVS)and supplementary food on Ag, Cd, and Zn bioaccumulation in the marine polychaete,Neanthes anrenaceodentata Lee, J.S;B.G. Lee;H. Yoo;C.H. Koh;S.N. Luoma https://doi.org/10.3354/meps216129
  18. Bull Environ. Contamin Toxicol v.29 Acute toxicity of silver to selected fish and invertebrates Lima, A.R;C. Curtis;D.E. Hammermeister;D.J. Call;T.A. Felhaber https://doi.org/10.1007/BF01606148
  19. Mar. Pollut. Bull v.31 Fate, biovailability and toxicity of silver in estuarine environments Luoma, S.N;Y.B. Ho;G.W. Bryan https://doi.org/10.1016/0025-326X(95)00081-W
  20. NTIS CONF-750920 The availability of sedimentbound cobalt, silver, and zinc to a deposit-feeding clam. In: Biological Implications of Metals in the Environment Luoma, S.N;E.A. Jenne;Wilung R.E(ed.);H.Drucker(ed.)
  21. Mar. Ecol. Prog. Ser v.12 Long-tern silver effects on the marine gastropod Crepidula fornicata Nelson, D.A;A. Calabrase;R.F. Greig;P.P. Yevich;S. Chang https://doi.org/10.3354/meps012155
  22. Environ. Sci. Technol v.26 Anthropogenic silver in the southern California bight: a new tracer of sewage in coastal waters Sanudo Willhelmy, S;R. Flegal https://doi.org/10.1021/es00035a012
  23. Can. J. Fish. Aquat. Sci v.38 Variations in the correlation of body size with concentrations of Cu and Ag in the bivalve Macoma balthica Strong, C.R;S.N. Luoma https://doi.org/10.1139/f81-146
  24. Mar. Ecol. Prog. Ser v.178 Bioavailability of dissolved and sediment-bound metals to a marine deposit-feeding polychaete Wang, W.X;I. Stupakoff;N.S. Fisher https://doi.org/10.3354/meps178281