Applied Microscopy

Korean Society of Microscopy (한국현미경학회)
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Effects of Squalene on SOD Activity and Histological Changes in Liver Toxicity Induced by Cadmium

Cadmium으로 유발된 간독성에서 SOD활성과 조직학적 변화에 대한 스쿠알렌의 효과

  • Choi, Young-Bok (Dept. of Biological Sciences, College of Natural Sciences, Chosun University) ;
  • Kim, Jong-Se (Dept. of Biological Sciences, College of Natural Sciences, Chosun University) ;
  • Kim, Jung-Sam (Dept. of Physical Therapy, Mokpo Science College) ;
  • Cho, Kwang-Pil (Dept. of Physical Therapy, Mokpo Science College) ;
  • Hwang, Koo-Yeon (Dept. of Clinical Pathology, Seokang College) ;
  • Park, Jung-Pyung (Animal Experiment Center, College of Medicine, Chosun University)
  • 최영복 (조선대학교 자연과학대학 생물과학부) ;
  • 김종세 (조선대학교 자연과학대학 생물과학부) ;
  • 김정삼 (목포과학대학 물리치료과) ;
  • 조광필 (목포과학대학 물리치료과) ;
  • 황구연 (서강정보대학 임상병리과) ;
  • 박정평 (조선대학교 의과대학 동물실험센타)
  • Published : 2002.09.01
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Abstract

This study was carried out to investigate the effect of squalene (SQ) on the mouse hepatotoxicity induced by cadmium. ICR male mouse weighting about 30 gm were injected $CdCl_2$ (5.0 mg/kg) and SQ (180 mg/kg) into intraperitoneal. At the 1, 2, 3, 4, 5, 6, 7 days, livers were treated with superoxide dismutase (SOD) activity and transmission electron microscopical method and then observed with electron microscope. The results obtained were summarized as follows: SOD activity in the liver, Group A was higher than in normal. Group B was lower than in Group A. In the histological observation, nucleus of Group A showed irregular shape. Inner cavity of mitochondria swellen and development of cristae weakened. Swelling of Lamellae of rough endoplasmic reticulum (RER) showed. Nucleus of group B showed normal shape. Typical lamellae of RER were observed. These results described above treatment of SQ decreased the hepatotoxicity of the $CdCl_2$ and SOD activity in the mouse liver, and then it suggests SQ may be effective for the recovery of hepatic cell.

[ $CdCl_2$ ]이 간에 미치는 독성에 관한 Squalene의 효과를 알아보기 위하여 30 mg 내외의 웅성 ICR계 mouse에 체중 kg당 5.0 mg의 $CdCl_2$와 체중 kg당 180.0 mg의 SQ를 복강투여하여 1, 2, 3, 4, 5, 6, 7일째에 절취한 간조직을 SOD 활성도와 투과전자현미경법을 이용하여 관찰한 결과 다음과 같은 결론을 얻었다. 간조직에서 SOD 활성도는 Group A가 정상군에 비해 증가를 보였고, Group B는 Group A에 비해 약간의 감소를 나타내었다. 조직학적 관찰에서 Group A의 핵은 불규칙한 모양이 관찰되었고, 미토콘드리아의 내강 팽대와 cristae 파괴가 나타났으며, 조면소포체의 수조 팽대가 관찰되었다. Group B의 핵은 원형을 나타내었으며 미토콘드리아는 약간 팽대되었지만 정상적인 형태를 보였고, 조면소포체의 전형적인 층판구조가 관찰되었다. 이상의 결과를 종합하면 SQ 처치가 SOD 활성을 감소시키고 mouse 간세포에 미치는 카드뮴의 독성을 감소시켜 간세포의 회복에 효과가 있는 것으로 사료된다.

Keywords

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