Extractives from the Bark of Sophora japonica L

  • Park, Youngki (Dept. of Forest Resources, Dongguk University) ;
  • Lee, Hak-Ju (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Choi, Don-Ha (Div. Wood Chemistry & Microbiology, Korea Forest Research Institute) ;
  • Kwon, Yeong-Han (Nambu Forest Experiment Station, KFRI) ;
  • Oh, Jung-Soo (Dept. of Forest Resources, Dongguk University)
  • Received : 2002.06.19
  • Accepted : 2002.08.07
  • Published : 2002.09.26

Abstract

This study was carried out to investigate the constituents of Sophora japonica(Leguminosae) bark. To isolate compounds, bark was extracted with ethanol and then partitioned with hexane, dichloromethane, ethylacetate and butanol successively. After partitioned, DCM fraction was subjected to column chromatography with various solvent system in silica gel and/or Sephadex LH-20. Structures were elucidated by spectroscopic methods including MS, 1H, 13C and 2D-NMR experiments. Four compounds were isolated from the bark and identified as 3',7-dihydroxy-4'-methoxyisoflavone (cyclosin), puerol A, maackiain-3-O-𝛽-D-glucopyranoside (trifolirhizin), and 4', 5, 7-trihydroxyisoflavone (genistein). Among these compounds, cyclosin and trifolirhzin were first isolated from S. japonica.

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