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The Korean Society of Phycology (한국조류학회(藻類))
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Phylogenetic Relationship of Microcystis (Cyanophyceae) Based on Partial 16S rRNA Gene Sequences in Korea

16S rRNA 유전자의 일부 염기서열에 기초한 한국산 Microcystis의 계통 유연관계

  • Kim, Jong-In (Department of Life Science, Chongju University) ;
  • Lim, Jong-Hun (ChungCheongBuk-Do Institute of Health & Environmental Research) ;
  • Lee, Jae-Wan (Department of Life Science, Chongju University) ;
  • Lee, Hae-Bok (Department of Life Science, Chongju University)
  • 김종인 (청주대학교 생명과학과) ;
  • 임종헌 (충청북도 보건환경연구원) ;
  • 이재완 (청주대학교 생명과학과) ;
  • 이해복 (청주대학교 생명과학과)
  • Published : 2002.09.30
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Abstract

Partial 16S rRNA gene sequences of seven cyanophycean strains from the National Instiute of Environmental Research of Korea - Microcystis aeruginosa, M. aeruginosa f. aeruginosa, M. ichthyoblade, M. viridis, Anabaena flos-aquae, and Oscillatoria sancta - were analyzed and the phylogenetic relationship of Microcystis among Cyanophyceae were evaluated. Based on sequence analysis results, Microcystis is monophyletic, the clade of which supported 100% bootstrap tress, and distinguished clearly from the other taxa. Therefore, the partial 16S rRNA gene sequences can be a useful and efficient tool for distinguishing Microcystis from other cyanophycean without axenic culture or cloning.

Keywords

References

  1. Bittencourt-Oliveira M.C., Oliveira M.C. and Bolch C.J.S. 2001. Genetic variability of brazilian stains of the Microcystis aeruginosa complex (Cyanobacteria/Cyanophyceae) using the phycocyanin intergenic spacer and flanking regions (cpcBA). J. Phycol. 37: 810-818 https://doi.org/10.1046/j.1529-8817.2001.00102.x
  2. Doyle J.J. and Doyle J.L. 1987. A rapid DNA isolation procedure for small quantities of fresh leaf tissue. Phytochem. Bull. 19: 11-15
  3. Felsenstein J. 1999. PHYLIP Phylogeny inference package, ver. 3.573c. Distributed by the autor, Department of Genetics, University of Washington, Seattle.
  4. Hall T.A. 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids. Symp. Ser. 41: 95-98.
  5. Ishida T., Yokata A. and Sugiyama J. 1997. Phylogenetic relationships of filamentous cyanobacterial taxa inferred from 16S rRNA sequence divergenece. J. Gen. Appl. Microbiol. 43: 237-241. https://doi.org/10.2323/jgam.43.237
  6. Kumar S., Tamura K., Jakobsen I.B. and Nei M. 2001. MEGA2: Molecular Evolutionary Genetics Analysis software. Arizona State University, Tempe, Arizona, USA.
  7. Lee W.J. and Bae K.S. 2001. The phylogenetic relationship of several oscillatiorian cyanobaceria, forming blooms Daecheong Reservoirs, based on partial 16S rRNA gene sequences. J. Microbiol. Biotechnol. 11: 504-507.
  8. Lee J.A., Choi A.R. and Watanabe M. 1997. Taxonomic implications of the genus Microcystis (Cyanophyceae) from the Naktong river. Algae 12: 167-176.
  9. Nelissen B., De Baer R., Willmontte A. and De Wachter R. 1996. Phylogenetic relationships of nonaxenic filamentous cyanobacterial strains based on 16S rRNA sequence analysis. J. Mol. Evol. 42: 194-200. https://doi.org/10.1007/BF02198845
  10. Nubel U., Garcia-Piche F. and Muyzer G. 1997. PCR primers to amplify 16S rRNA genes from cyanobacteria. Appl. Environ. Microbiol. 63: 3327-3332.
  11. Park H.K. 1998. Physio-ecological study on Korean freshwater cyanobacteria, Microcystis spp. Diss. Pap. Ph. D. KNU. 146 pp.
  12. Reynolds C.S., Jaworski G.H.M., Cmiech H.A. and Leedale G.F. 1981. On the annual cycle of the blue-green alga Microcystis aeruginosa. Kutz. Emend. Elenkin. Phil. Trans. Roy. Soc. Lond. B. 293: 419. https://doi.org/10.1098/rstb.1981.0081
  13. Otsuka S., Suda S., Li R., Watanabe M., Oyaizu H., Masumoto S. and Watanabe M.M. 1998. 16S rDNA sequences and phylogenetic analyses of Microcystis strains with and without phycoerythrin. FEMS Microbiol. Lett. 164: 119-124. https://doi.org/10.1111/j.1574-6968.1998.tb13076.x
  14. Otsuka S., Suda S., Li R., Watanabe M., Oyaizu H., Masumoto S. and Watanabe M.M. 1999. Phylogenetic relationships between toxic and non-toxic strains of the genus Microcystis based on 16S to 23S interanal transcrived spacer sequence. FEMS Microbiol. Lett. 172: 15-21. https://doi.org/10.1111/j.1574-6968.1999.tb13443.x
  15. Thompson J.D., Higgins, D.G. and Gibson, T.J. 1994. CLUSTAL W: improving the senstivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice. Nucleic Acids Res. 22: 4673-4680. https://doi.org/10.1093/nar/22.22.4673
  16. Watanabe M.F. and Harada K.I. 1993. Toxic water bloom of blue-green algae: biological and chemical characteristics. Jpn. J. Limnol. 54: 225-243. https://doi.org/10.3739/rikusui.54.225
  17. Watanabe M.M. and Nozaki H. 1994. NIES-collection list of strains. 4th ed. pp. 32.
  18. Yeo I.H., Han S.K., Yu J.H. and Bai D.H. 1998. Isolation of novel alkalophilic Bacillus alcalophilus subsp. YB380 and the characteristics of its yeast cell wall hydrolase. J. Microbiol. Biotechnol. 8: 501-508.

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