Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Effects of Gamibojungikki-tang on Total Protein Synthesis of Cultured Spinal Sensory Neurons Damaged by GLUCOSE OXIDASE

가미보중익기탕이 GLUCOSE OXIDASE에 의해 손상된 배양 척수감각신경세포의 총단백질 합성량에 미치는 영향

  • Ho Lee Chang (Department of Physiology, College of Oriental Medicine, WonKwang University) ;
  • Beam Kwon Kang (Department of Physiology, College of Oriental Medicine, WonKwang University) ;
  • Ho Jang Seung (Department of Physiology, College of Oriental Medicine, WonKwang University) ;
  • Sun Song Yong (Department of Oriental Rehabilitation Medicine, Collage of Oriental Medicine, WonKwang University) ;
  • Gon Ryu Do (Department of Physiology, College of Oriental Medicine, WonKwang University)
  • 이창호 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 장승호 (원광대학교 한의과대학 생리학교실) ;
  • 송용선 (원광대학교 한의과대학 재활의학과) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Published : 2002.02.01
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Abstract

In order to clarify the neuroprotective effect of Gamibojungikki-tang (GBJIKT) water extract on cultured mouse spinal sensory neuron damaged by glucose Oxidase (GO), MTT [3-(4,5-dimethylthiazole-2-yl) -2,5-diphenyltetrazolium bromide] assay and SRB (Sulforhodamine B) assay were carried out after the cultured mouse spinal sensory neuron were preincubated with various concentrations of GBJIKT water extract for 3 hours prior to exposure of GO. Cell viability of cultured mouse spinal sensory neurons exposed to various concentrations of GO for 8 hours was decreased in a dose-dependent manner. MTT50 values were 45 mU/ml GO. Cultured mouse spinal sensory neurons in the medium containing various concentration of GO for 8 hours showed decreasing of total protein synthesis. GO was toxic on cultured spinal sensory neurons. Pretreatment at GBJIKT water extract for 3 hours following GO prevented the GO-induced neurotoxicity such as decreasing of total protein synthesis. These results suggest that GO shows toxic effect on cultured spinal sensory neurons and GBJIKT water extract is highly effective in proecting the neurotoxicity induced by GO.

Keywords

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