Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
권호 표지

Screening of the Acetylcholinesterase Inhibitors from Water Extracts of the Medicinal Plants

한약재 물 추출물의 아세틸콜린에스테라제 활성 억제 검색

  • Lee Hyung Chul (Department of pathology, College af Oriental Medicine. Wonkwang University) ;
  • Hwang Sang-Gu (Department of pathology, College af Oriental Medicine. Wonkwang University) ;
  • Lee Young Chan (Department of pathology, College af Oriental Medicine. Wonkwang University) ;
  • Kim Dae Gun (Department of pathology, College af Oriental Medicine. Wonkwang University) ;
  • Park Young Jun (Department of pathology, College af Oriental Medicine. Wonkwang University) ;
  • Kim Tae Eun (Department of Physiology, College af Oriental Medicine. Sangji University) ;
  • Kim Moung Dong (Department of Physiology, College af Oriental Medicine. Sangji University) ;
  • Jeon Byung Hun (Department of pathology, College af Oriental Medicine. Wonkwang University)
  • 이형철 (원광대학교 한의과대학 병리학교실) ;
  • 황상구 (원광대학교 한의과대학 병리학교실) ;
  • 이영찬 (원광대학교 한의과대학 병리학교실) ;
  • 김대근 (원광대학교 한의과대학 병리학교실) ;
  • 박영준 (원광대학교 한의과대학 병리학교실) ;
  • 김태은 (상지대학교 한의과대학 생리학교실) ;
  • 김명동 (상지대학교 한의과대학 생리학교실) ;
  • 전병훈 (원광대학교 한의과대학 병리학교실)
  • Published : 2002.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

Over the past decade there has been a resurgence of interest in botanical products for their medicinal uses. This present study was designed to screen medicinal plants for the treatment of brain diseases such as Alzheimer's disease or aging. We tested the effects of the water extracts from 41 species medicinal plants on acetylcholinesterase (AChE) inhibition and antioxidant activity in vitro. Among them, Euodia rutaecarpa, Rubus coreanus, Achyranthes japonica, Morus alba, Salvia miltiorrhiza, Liriope platyphylla, and Rheum palmatum showed relatively AChE inhibition potency over 80%. Among the 7 medicinal plants investigated, E. rutaecarpa showed an 25%, 65%, and 93% inhibitions on AChE activity at 2.5, 12.5, and 25 ㎍/㎖ of the extract, respectively and was found to be one of most potent AChE inhibitor. The water extracts from 4 species (E. rutaecarpa, R. coreanus, A. iaponica, and M. alba) were tested on their antioxidant capacities using radical scavenging effect against ABTS/sup ㆍ+/. Among the 4 medicinal plants investigated, both R. coreanus and E. rutaecarpa showed significant antioxidant capacity. Therefore, E. rutaecarpa and R. coreanus are expected to ameliorate the clinical symptoms in Alzheimer's disease due to significant AChE inhibition and radical scavenging effect.

Keywords

References

  1. 醫學入門(全五卷中二) v.5 이천
  2. Progress Report on Alzheimer's disease (NIH Publication 95-3994). National Institute on Aging.
  3. Arch. Neurol. v.42 Diagnosis of Alzheimer's disease. Khachaturian, Z.S. https://doi.org/10.1001/archneur.1985.04060100083029
  4. Acta Psychiat. Scand. v.76 The prevalence of dementia: A quantitative integration of the literature. Jorm, A.;Korten, A.;Henderson, A.
  5. Lancet v.2 Neurotransmitters and C.N.S. disease dementia. Rossor, M.N.
  6. Rev. Neurol. v.155 Alzheimer's disease: lesions and their progression. Duyckaerts, C.;Colle, M.A.;Delatour, B.;Hauw, J.J.
  7. Arch. Neurol. v.44 Cholinesterase in cerebrosponal fluid. A longitudinal study in Alzheimer disease. Elble, R.;Giavobini, E.;Scarsella, G.F. https://doi.org/10.1001/archneur.1987.00520160041012
  8. Science v.219 Alzheimer's disease; A disorder of cortical cholinergic innervation. Coyel, J.T.;Price, D.L.;Long, M.R. https://doi.org/10.1126/science.6338589
  9. Mech. Aging Dev. v.122 Acetylcholinesterase in Alzheimer's disease. Talesa, V.N. https://doi.org/10.1016/S0047-6374(01)00309-8
  10. Neurobiol. Disease v.5 Possible causes of Alzheimer's disease: Amyloid fragments, free radicals, and calcium homeostasis. Holscher, C. https://doi.org/10.1006/nbdi.1998.0193
  11. Drug v.53 Cognitive enhancement therapy for Alzheimer's disease. The way forward. Parnetti, L.;Senin, U.;Mecocci, P. https://doi.org/10.2165/00003495-199753050-00003
  12. J. Nat. Pro. v.58 Acetylcholinesterase inhibition by territrem B derivatives. Peng, F.C. https://doi.org/10.1021/np50120a006
  13. Kor. J. Pharmacogn. v.27 An acetylcholinesterase inhibitor isolated from Corydalis Tuber and its mode of action. Hwang, S.Y.;Chang, Y.P.;Byun, S.J.;Jeon, M.H.;Kim, Y.C.
  14. Brain Res. v.760 Neuroprotective effects of the antioxidant LY231617 and NO synthase inhibitors in global cerebral ischaemia. O'Neill, M.;Hicks, C.;Ward, M.;Panetta, J.A. https://doi.org/10.1016/S0006-8993(97)00293-X
  15. Br. J. Pharmacol. v.119 Inhibition of nitric oxide synthase by 1-(2-trifluoromethylphenyl) imidazole (TRIM) in vitro: Antinociceptive and cardiovascular effects. Handy, R.L.C.;Harb, H.L.;Wallace, P.;Gaffen, Z.;Whitehead, K.J.;Moore, P.K. https://doi.org/10.1111/j.1476-5381.1996.tb16003.x
  16. 實用中醫內科學 方藥中(主編)
  17. 增補 萬病回春 공정현
  18. 東洋醫學大辭典 謝觀
  19. 備急千金要方(卷四十) v.14 孫思邈
  20. 中醫老年病學 袁立人
  21. Biochem. Pharmacol. v.7 A new and rapid colorimetric determination acetyl -cholinesterase activity. Ellman, G.L.;Coutney, K.D.;Andres, V.Jr.;Featherstone, R.M. https://doi.org/10.1016/0006-2952(61)90145-9
  22. Meth. Enzymol. v.234 Total antioxidant status in plasma and body fluids. Rice-Evans, C.;Miller, N.J. https://doi.org/10.1016/0076-6879(94)34095-1
  23. 韓方成人病學會誌 v.3 三和散이 腦組織의 acetylcholine esterase 및 monoamine oxidase 活性에 미치는 影響. 徐榮浚;申鉉喆;尹哲浩;鄭智天;申億變
  24. 東醫神經精神科學會誌 v.8 七福飮이 노화 백서 뇌조직의 생화학적 변화에 미치는 영향. 손정석;류영수
  25. 大韓韓醫學會誌 v.19 定志丸이 腦組織의 生化學的 變化와 神經細胞의 損傷에 미치는 實驗的 硏究. 崔龍埈;成彊慶;文炳淳
  26. Kor. J. Pharmacogn. v.28 Screening of the acetylcholinesterase inhibitors from medicinal plants. Lee, B.H.;Choi, B.W.;Ryu, G.S.;Lee, E.S.;Kang, K.J.;Hwang, D.Y.;Hong, N.D.