동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제16권5호
  • /
  • Pages.1016-1019
  • /
  • 2002
  • /
  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

조구등이 Glucose Oxidase로 손상된 대뇌신경세포에 미치는 효과

Effect of Ramulus et uncus uncariae on Glucose Oxidase-Induced Toxicity in Cultured Cerebral Neurons

  • Kim Hyeong Soo (School of Medicine, Wonkwang University) ;
  • Lee Yong Suk (School of Medicine, Wonkwang University) ;
  • Oh Suk Kyu (School of Medicine, Wonkwang University) ;
  • Lee Kang Chang (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Geon Mok (Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Jeong (Wonkwang health Science College) ;
  • Lee Sang Bork (Wonkwang health Science College) ;
  • Kim Jong Ho (Wonkwang health Science College) ;
  • Yu Jun Ki (College of Oriental Medicine. Wonkwang University) ;
  • Kang Young Seong (College of Oriental Medicine. Wonkwang University) ;
  • Kim Sung Soo (College of Oriental Medicine. Wonkwang University) ;
  • Song Ho Jun (College of Oriental Medicine. Wonkwang University) ;
  • Park Seung Taeck (School of Medicine, Wonkwang University)
  • 발행 : 2002.10.01
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초록

To examine the cytotoxic effect of glucose oxidase(GO) in cultured mouse cerebral neurons, cytotoxicity was measured by MTT assay after cultured nerve cells were incubated for 3 hours in the media containing 1 ～ 60mU/ml concentrations of GO. In addition, the neuroprotective effect of Ramulus et uncus uncariae(REUU) was determined by MTT assay in these cultrures. Cell viability was remarkably decreased in a dose- and time-dependent manner after cultured mouse cerebral neurons were exposed to 30mU/ml GO for 3 hours. In the neuroprotective effect of REUU on GO-induced toxicity, REUU blocked the GO-mediated neurotoxicity in these cultures. From above the results, it suggests that GO is toxic in cultured mouse cerebral neurons and selective herb extract such as REUU is effective in prevetion of the neurotoxicity induced by GO.

키워드

참고문헌

  1. Neuroreport v.3 Pre-treatmnet with a $Ca^{2+}$ channel antagonist facilitates vestibular compensation. Darlington C.L.;Smith P.F.
  2. Nutr Revi v.53 The role of phenolics, conjugated linoleic acid, carnosine, and pyrroloquinoline quinone as nonessential dietary antioxidants. Decker E.A.
  3. Nature v.336 NMDA receptors activate the arachidonic acid cascade system in striatal neurons. Dumuis A;Sebben M;Haynes L;Pin J-P;Bockaert J
  4. Biochem Pharmacol v.15 Metabolic studies of allopurinol, an inhibitor of xanthine oxidase. Elion G.B.;Kovensky A.;Hitchings G.H.;Metz E.;Rundles R.W.
  5. J Biochem v.259 Iron-catalyzed hydroxyl radical formation : Stringent requirement for free iron coordination site. Graf E;Mahoney JR;Bryant RG;Eaton Jw
  6. Cent Nerv System Trauma v.3 Role of lipid peroxidation in post-traumatic spinal cord degeneration. Hall E;Braughler JM
  7. J. Neurochem v.59 Reactive oxygen species and the central nervous system. Halliwell B.
  8. Agric Biol Chem v.47 Antioxidative effect of consttuents of rosemary(Rosemarinus of ficinalis L.) and their derivatives. Natani, R.;Nakatani, N.;Fuwa, H.
  9. J Neurosci Res v.29 Oligodendroglial cell death induced by oxygen radicals and its protection by catalase. kim, Y.S.;Kim, S.U.
  10. Trends in Food Sci Tech v.6 Spices as antioxidants. Madesen HL;Bertelsen G
  11. J Neurosci Res v.37 Oxygen radical-induced neurotoxicity in spinal cord neuron cultures. Michikawa M.;Lim K. T.;McLarnon J. G.;Kim S. U.
  12. J Immun Methods v.65 Rapid colorimetric assay for the cellular growth and survival: Application to proliferation and cytotoxic assays. Mosmann T
  13. J Neurosci v.10 Excitatory amino acid and free radical formation may cooperate in the genesis of ischemia- induced neuronal damage. Pellegrini-Giampietro DE;Cherici G;Alesiani M;Carla V;Morroni F.
  14. Stroke v.15 The protective effects of combined administration of anti-oxidants and perfluoro- chemicals on cerebral ischemia Suzuki J;Fujimoto S;Oba M
  15. Proc Natl Acad Sci U.S.A. v.89 A major inducer of anticarcinogenic protective enzymes from broccoli: Isolation and elucidation of structure. Zhang Y;Talaly P;Cho CG;Posner GH