Journal of Physiology & Pathology in Korean Medicine (동의생리병리학회지)

The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine (대한동의생리학회·한의병리학회)
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Inhibitory Effect on Melanogenesis of Radix Glycyrrhizae Water Extract

감초 물추출물의 멜라닌 형성 억제효과

  • Mun Yeun Ja (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Kim Jin (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lim Nan Young (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Lee Seung Yon (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Seop Gwak (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Hwang Chung Yeon (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University) ;
  • Woo Won Hong (Department of Herbal Resources, Professional Graduate School of Oriental Medicine, Wonkwang University)
  • 문연자 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 김진 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 임난영 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 이승연 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 곽섭 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 황충연 (원광대학교 한의학전문대학원 한약자원개발학과) ;
  • 우원홍 (원광대학교 한의학전문대학원 한약자원개발학과)
  • Published : 2002.12.01
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Abstract

This study was conducted to evaluate the effects of Glycyrrhizae Radix water extract, known as depigmenting agent, on melanin biosynthesis in cellular level. The inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis was identified by mushroom tyrosinase assay, To determine whether Glycyrrhizae Radix water extract suppress melanin synthesis in cellular level, B16 mouse melanoma cells were cultured in the presence of different concentrations of Glycyrrhizae Radix water extract. The maximum concentration of Glycyrrhizae Radix water extract that was not inhibitory to growth of the cells was 2 mg/ml. At that concentration, melanin synthesis was significantly inhibited without cytotoxicity after 5 days, compared with untreated cells. The treatment with Glycyrrhizae Radix water extract reduced tyrosinase and DOPAchrome tautomerase activity in a dose-dependent manner. These results suggest that the inhibitory effect of Glycyrrhizae Radix water extract on melanogenesis is due to the suppression of tyrosinase and DOPAchrome tautomerase activity.

Keywords

References

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