동의생리병리학회지 (Journal of Physiology & Pathology in Korean Medicine)

  • 제16권6호
  • /
  • Pages.1117-1121
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  • 2002
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  • 1738-7698(pISSN)
  • /
  • 2288-2529(eISSN)
대한동의생리학회·한의병리학회 ( The Physiological Society of Korean Medicine and The Society of Pathology in Korean Medicine)
권호 표지

삼종 지황 추출물이 배양 심근세포에 미치는 영향

Effects of three kinds of Radix Rehmanniae Water Extract in Cultured Rat Myocardial Cells

  • 황인진 (원광대학교 한의과대학 생리학교실) ;
  • 권강범 (원광대학교 한의과대학 생리학교실) ;
  • 조현익 (원광대학교 한의과대학 생리학교실) ;
  • 민영기 (원광대학교 한의과대학 생리학교실) ;
  • 허재혁 (원광대학교 한의과대학 생리학교실) ;
  • 김구환 (원광대학교 한의과대학 생리학교실) ;
  • 류도곤 (원광대학교 한의과대학 생리학교실)
  • Hwang In Jin (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kwon Kang Beom (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Cho Hyun Ik (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Min Young Gi (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Heo Jae Hyuk (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Kim Gu Hwan (Department of Physiology, College of Oriental Medicine, Wonkwang University) ;
  • Ryu Do Gon (Department of Physiology, College of Oriental Medicine, Wonkwang University)
  • 발행 : 2002.12.01
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초록

To test the protective effect of herbal medicine on myocardial damage against oxygen free radical-induced myocardiotoxicity, cytotoxicity was examined using MTT, Beating rate and DNA synthesis assay in the presence of water extract of three kinds of Radix Rehmanniae. Myocardial toxicity was evaluated in neonatal rat myocardiocytes in cultures. The results of these experiments were obtained as follows : Xanthine oxydase/hypoxanthine resulted in a decrease in viability, beating rate and DNA synthesis in cultured myocardial cells. Radix Rehmanniae Recens(生地黃, RRR) water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in beating rate. Radix Rehmanniae Preparat(熟地黃, RRP) water extract shows effects of protection from the cardiocyte toxicity induced by xanthine oxydase/hypoxanthine treatment such as increases in DNA synthesis. These results show that xanthine oxydase/hypoxanthine elicits toxic effects in cultured myocardial cells derived from neonatal rat, and suggest that water extract of three kinds of Radix Rehmanniae is very effective in the prevention of xanthine oxydase/hypoxanthine-induced cardiotoxicity.

키워드

참고문헌

  1. 韓藥學Ⅱ 陸昌洙(외)
  2. 漢藥臨床應用 李尙仁(외)
  3. 申氏本草學 申佶求
  4. 本草維新 辛民敎
  5. J. Biol. Chem. v.245 Quantitative aspects of the production of superoxide anion radical anion radical by milk xanthine oxidase. Fridovich I.
  6. J. Biol. Chem. v.252 Liposome oxidation and erythrocyte lysis by enzymatically generated superoxide and hydrogen peroxide. Killogg E. W.;Fridovich I.
  7. Proc. Roy. Soc. London A. v.147 The catalytic decomposition of hydrogen peroxide by anion salts. Harber, F.;Weiss, J.
  8. J. Biol. Chem. v.259 Iron-catalyzed hydroxyl radical formation: Stringent requirement for free iron coordination site. Graf E.;Mahoney JR;Bryant RG;Eaton JW
  9. Int. J. Neurosci v.57 Oxygen free radicals and brain dysfunction. Jesberger J. A.;Richardson J. S.
  10. J. Cereb Blood Flow Metab. v.13 Basic FGF, NGF, and IGFs protect hippocampal and cortical neurons against iron-induced degeneration. Zhang Y.;Tatsuno T.;Carney J. M.;Mattson M. P.
  11. FASEB J. v.4 Role of oxygen free radicals in carcinogenesis and brain ischemia. Floyd R. A.
  12. J. Immunol. Methods v.65 Rapid colirimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assay. Mosmann T.
  13. Chem. Pharm. Bull. v.35 no.1 Effect of adriamycin on cultured mouse embrio myocardial cells. K. Takahashi;Y. Fujita;T. Mayumi;T. Hama;T. Kishi
  14. J. Korean Oriental Med. v.21 no.2 Effects of Myrrha Water Extract on Rat Myocardial Cells in Cultures. Kwon K. B.;Jo H. I.;Kim G. H.;Kim S. B.;Lee H. S.;Hwang W. J.; Park S. T.;Ryu D. G.
  15. Korean Journal of Oriental Medical Physiology & Pathology v.15 no.2 Effects of Dansamyeum Water Extract on Heart Beating Rate in Cultured Mouse Myocardial Cells. Son C. S.;Kwon K. B.;Kim S. B.;Lee H. S.;Seo E. H.;Lee H. S.;Ryu D. G.
  16. Korean Journal of Oriental Medical Physiology & Pathology v.15 no.4 Effects of Dansam-yeum Water Extract on LDH Activity of Myocardial Cells damaged by Reactive Oxygen Species. Son C. S.;Kwon K. B.;Jung J. S.;Hwang I. J.;Kim W. K.;Kim H. C.; Oh K. S.;Lee H. S.;Ryu D. G.