DOI QR코드

DOI QR Code

In Vitro Inhibitory Activity of Cow Urine and Dung to Fusarium solani f. sp. cucurbitae

  • Basak, A.B. (Department of Botany, University of Chittagong) ;
  • Lee, Min-Woong (Department of Applied Biology, Dongguk University) ;
  • Lee, Tae-Soo (Department of Biology, University of Inchon)
  • Published : 2002.03.31

Abstract

This paper deals with the study on comparative efficacy and in vitro activity of cow urine and cow dung for controlling root rot disease of cucumber caused by Fusarium solani f. sp. cucurbitae Snyder & Hansen following slide germination and mycelial growth inhibition tests. Results showed that both germination of conidia and the percentage inhibition of mycelial growth decreased or suppressed and varied greatly with respect to different hour and days of incubation and kind of bio-matters. In between two bio-matters cow urine was found more effective than that of cow dung in conidial germination. No germination of conidia was recorded after one hour of incubation in any medium whereas in cow urine germination of conidia was not also observed even after 2 hours of incubation. After 7 hours of incubation out of 200 conidia of F. solani f. sp. cucurbitae, 28 in cow urine and 64 in cow dung were germinated while in control a total germinated conidia was 185. In case of percentage inhibition of conidial germination the highest percentage(100%) was recorded in cow urine after 2 hours of incubation followed by 3 hours(96.0%), 4 hours(91.0%) and 6 hours(89.4%). During the test on inhibition of mycelial growth, the highest percentage(62.8%) was recorded in cow urine potato dextrose agar(CUPDA) medium tested after 4 days of incubation, followed by 3 days(60.5%), 5 days(56.5%) and 2 days(55.0%). In this test cow dung potato dextrose agar(CDPDA) had less efficacy in suppression of the percentage inhibition of mycelial growth.

Keywords

References

  1. Agrios, G. N. 1988. Plant Pathology. Academic Press, Inc., New York, U.S.A. Third Edition
  2. Anonymous, 1998. List of Plant Diseases in Korea. Korean Society of Plant Pathology. Third edition
  3. Ashrafuzzaman, M. H. 1976. Laboratory Mannual of Plant Pathology, lst edition, Zaman Manzil, Iqbal Nagar, Khulna, Bangladesh
  4. Bakker, P. A H. M., Van Peer, R. and Schippers, B. 1990. Suppression of soil borne plant pathogens by fluorescent pseudomonads: mechanisma and prospects. In; Beemster ABR, ed. Biotic interaction and soil borne diseases. Elsevier, pp. 217-230
  5. Basak, A. B. and Paul, P. 1999. Effects of some plant extracts on fruit rot fungal pathogens of chilli. Chittagong University J. Sci. 3: 129-135
  6. Basak, A. B. and Lee, M. W. 2001a. Efficacy of cow dung in controlling root rot and Fusarium wilt disease of cucumber plants. Abstract published in the 2001 Korean Society of Plant Pathology Annual meeting and International Conference, held on the 25-30th October, Kyongju, Korea. pp. 49
  7. Basak, A. B. and Lee, M. W. 2001b. Comparative efficacy and in vitro activity of cow urine and cow dung for controlling Fusarium wilt of cucumber. Abstract published in the 2001 Korean Society of Plant Pathology Annual meeting and International Conference, held on the 25-30th October, Kyongju, Korea. pp. 49
  8. Boer, M. de. Sluis, Intse Van der, Loon Leendert, C., Bakker, P. A H. M. 1999. Combining fluorescent Pseudomonas spp. Strains to enhance suppression of Fusarium wilt of radish Eur. J. Plant Pathol. 105: 201-210
  9. Booth, C. and Waterson, J. M. 1964. Fusarium solani. C. M. I. Description of Pathogenic Fungi and Bacteria. No. 29, Commonwealth Mycological Institute, Ferry lane, Kew, Surrey, London, U.K.
  10. Cho, W D., Kim, W G., Jee, H. 1., Choi, H. S., Lee, S. D. and Choi, Y. C. 1997. Compendium of vegetable diseases with colour plates. Plant Pathology Division, Dept. of Crop Protection, National Institute of Agril. Science and Technology, Suwon 441-707, Korea, pp. 448
  11. Datnoff, L. E., Nemee, S. and Pernezny, K. 1995. Biological control of Fusarium crown rot and root rot of tomato in Florida using Trichoderma harzianum and Glomus intraradices. Biological Control 5: 527-431
  12. Holliday, P. 1970. Fusarium oxysporum f. sp. cucumerinum. C. M. I. Description of Pathogenic Fungi and Bacteria No. 215, Commonwealth Mycological Institute, Ferry Lane, Kew, Surrey, England
  13. Kim, W G., Cho, W D. and Jee, H. J. 1999. Occurrence of Sclerotia rot on cucurbitaceous vegetable crops in green houses. Korean J. Mycol. 27: 198-205
  14. Lee, J. T., Bae, D. W, Park, S. H., Shin, C. K., Kwak, Y. S. and Kim, H. K. 2001. Occurrence and biological control of post harvest decay in onion caused by fungi. Plant Pathology J. 17: 141-148
  15. Leeman, M., Den Ouden, F. M., Van Pelt, J. A, Coornelissen, C., Martamla-Garros, A, Bakker, P. A H. M. and Schippers, B. 1996. Suppression of Fusarium wilt of radish by co-inoculation of fluorescent Pseudomonas spp. And root colonizing fungi. Eur. J. Plant Pathol. 102: 21-31
  16. Walker, J. C. 1952. Diseases of vegetable crops. First edition. McGraw-Hill book Company, Inc., New York, Toronto, London

Cited by

  1. vol.32, pp.3, 2004, https://doi.org/10.4489/MYCO.2004.32.3.128
  2. Reduced cell wall degradation plays a role in cow dung-mediated management of wilt complex disease of chickpea vol.49, pp.7, 2013, https://doi.org/10.1007/s00374-013-0782-x
  3. Comparative Effect of Foliar Application of Cow Dung, Wood Ash and Benlate on the Disease Initiation and Development of Roselle (<i>Hibiscus sabdariffa</i> L.) Leaf Spot Disease Caused by <i>Coniella musaiensis</i> Var. <i>Hibisci</i>. in Makurdi, Central Nigeria vol.04, pp.08, 2016, https://doi.org/10.4236/gep.2016.48003