Mycobiology

  • 제30권1호
  • /
  • Pages.31-36
  • /
  • 2002
  • /
  • 1229-8093(pISSN)
  • /
  • 2092-9323(eISSN)
한국균학회 (The Korean Society of Mycology)
권호 표지
DOI QR코드

DOI QR Code

Selection and Efficacy of Soil Bacteria Inducing Systemic Resistance Against Colletotrichum orbiculare on Cucumber

  • Kwack, Min-Sun (Division of Bioscience and Technology, Korea University) ;
  • Park, Seung-Gyu (Department of Agricultural Technology, Dongbuhannong Chemical Co., Ltd.) ;
  • Jeun, Yong-Chull (Department of Plant Resources Science, Cheju National University) ;
  • Kim, Ki-Deok (Division of Bioscience and Technology, Korea University)
  • 발행 : 2002.03.31
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Soil bacteria were screened for the ability to control cucumber anthracnose caused by Colletotrichum orbiculare through induced systemic resistance(ISR). Sixty-four bacterial strains having in vitro antifungal activity were used for selecting ISR-inducing strains in cucumber. Cucumber seeds(cv. Baeknokdadagi) were sown in potting mixtures incorporated with the soil bacteria, at a rate of ca. $10^8$ cells per gram of the mixture. Two week-old plants were then transplanted into the steam-sterilized soil. Three leaf-stage plants were inoculated with a conidial suspension($5{\times}10^5$ conidia/ml) of C. orbiculare. Diseased leaf area(%) and number of lesions per $cm^2$ leaf were evaluated on third leaves of the plants, $5{\sim}6$ days after inoculation. Among 64 strains tested, nine strains, GC-B19, GC-B35, GK-B18, MM-B22, PK-B14, RC-B41, RC-B64, RC-B65, and RC-B77 significantly(P=0.05) reduced anthracnose disease compared to the untreated control. In contrast, some bacterial strains promoted susceptibility of cucumber to the disease. From the repeated experiments using the nine bacterial strains, GC-B19, MM-B22, PK-B14, and RC-B65 significantly(P=0.05) reduced both diseased leaf area(%) and number of lesions per $cm^2$ leaf in at lease one experiment. These strains with control efficacy of $37{\sim}80%$ were determined to be effective ISR-inducing strains.

키워드

참고문헌

  1. Adams, P. B. 1990. The potential of mycoparasites for biological control of plant diseases. Annu. Rev. Phytopathol. 28: 59-72 https://doi.org/10.1146/annurev.py.28.090190.000423
  2. Chang, S. H., Lee, J. Y, Kim, K. D. and Hwang, B. K. 2000. Screening for in vitro antifungal activity of soil bacteria against plant pathogens. Mycobiology 28: 190-192
  3. Cook, R. J. 1993. Making greater use of introduced microorganisms for biological control of plant pathogens Annu. Rev. Phytopathol. 31: 53-80 https://doi.org/10.1146/annurev.py.31.090193.000413
  4. Cook, R. J., Bruckart, W. L., Coulson, J. R., Goettel,M. S., Humber, R. A., Lumsden, R. D., Maddox, J. V, McManus, M. L., Moore, L., Meyer, S. E, Quimby, P. C, Stack, J. P. and Vaughn, J. L. 1996. Safety of microorganisms intended for pest and plant disease control: A framework for scientific evaluation. Biological Control 7: 333-351 https://doi.org/10.1006/bcon.1996.0102
  5. Dik, A. J., Koning, G. and Kohl, J. 1999. Evaluation of microbial antagonists for biological control ofBotrytis cinearea stem infeetion in cucumber and tomato. Eur. J Plant Pathol. 105: 115-122 https://doi.org/10.1023/A:1008623210258
  6. Han, D. Y, Coplin, D. L., Bauer, W. D. and Hoitink, H. A. J. 2000. A rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance. Phytopathology 90: 327-332 https://doi.org/10.1094/PHYTO.2000.90.4.327
  7. Hokeberg, M., Gerhardson, B. and Johnsson, L. 1997. Biologieal control of cereal seed-borne disease by seed bacterization with greenhouse-selected bacteria. Eur. J. Plant Pathol. 103: 25-33 https://doi.org/10.1023/A:1008681608400
  8. Hoffland, E., Pieterse, C. M. J., Bik, L. and Van Pelt, J. A. 1995. Induced systemic resistance in radish is not associated with accumulation of pathogenesis-related proteins. Physiol. Mol. Plant Pathol. 46: 309-320 https://doi.org/10.1006/pmpp.1995.1024
  9. Jeun, Y.-C., Park, K. and Kim, C.-H. 2001. Different mechanisms of induced systemic resistance and systemic acquired resistance aginst Colletotrichum orbiculare on the leaves of cucumber plants.Mycobiology 29: 19-26
  10. Kloepper, J. W. 1991. Development of in vivo assay for prescreening antagonists of Rhizoctonia solani in cotton. Phytopathology 81: 1006-1013 https://doi.org/10.1094/Phyto-81-1006
  11. Kloepper, J. W., Leong, J., Teintze, M. and Schroth, M. N. 1980. Enhanced plant growth by siderophores produced by plant growth-promoting rhizobacteria. Nature 286: 885-886 https://doi.org/10.1038/286885a0
  12. Kloepper, J. W., and Schroth, M. N. 1978. Plant growth-promoting rhizobacteria on radishes. In: Proceedings of the Forth International Conference on Plant Pathogenic Bacteria, Vol. 2. Station de Pathologie Vegetale et Phytobacteriologie, INRA, Angers (ed.). Gibert-Clarey, Tours, pp. 879-882
  13. Knoester, M., Pieterse, C. M. J., Bol, J. F. and Van Loon, L. C. 1999. Systemic resistance in Arabidopsis induced by rhizobacteria requires ethylene-dependent signaling at the site of application. Mol. Plant-Microbe Interact. 12: 720-727 https://doi.org/10.1094/MPMI.1999.12.8.720
  14. Kuc, J. 1995. Induced systemic resistance-An overview. Pp 169-175. In: Induced Resistance to Disease in Plants. R. Hammerschmidt and J. Kuc, eds. Kluwer Academic Publishers
  15. Kumar, B. S. D. 1998. Disease suppression and crop improvement through fluorescent pseudomonads isolated from cultivated soils. World J. Microbiol. Biotech. 14: 735-741 https://doi.org/10.1023/A:1008822200618
  16. Kwack, M. S. 2001. Selection and efficacy of soil bacteria inducing systemic resistance against Colletotrichum orbiculare in cucumber plants. M.S. Thesis, Korea University, Seoul, Korea. 73pp.
  17. Liu, L., Kloepper, J. W. and Tuzun, S. 1995. Induction of systemic resistance in cucumber against Fusarium wilt by plant growth promoting rhizobacteria: Duration of protection and effect of host resistance on protection and root colonization. Phytopathology 85: 1064-1068 https://doi.org/10.1094/Phyto-85-1064
  18. Mathre, D. E., Cook, R. J. and Callan, N. W. 1999. From discovery to use: Traversing the world of commercializing biocontrol agents for plant disease control. Plant Dis. 83: 972-983 https://doi.org/10.1094/PDIS.1999.83.11.972
  19. Meera, M. S., Shivanna, M. B., Kageyama, K. and Hyakumachi, M. 1995. Persistence of induced systemic resistance in cucumber in relation to root colonization by plant growth promoting fungal isolates. Crop Protec. 14: 123-130 https://doi.org/10.1016/0261-2194(95)92866-L
  20. Moenne-Loccoz, Y., Naughton, M., Higgins, P., Powell, J., Connor, B. and O'Gara, F. 1999. Effect of inoculum preparation and formulation on survival and biocontrol efficacy of Pseudomonas fluorescens F113. J. Appl. Microbial. 86: 108-116 https://doi.org/10.1046/j.1365-2672.1999.00640.x
  21. Moline, H., Hubbard, J. E., Karns, J. S., Buyer, J. S. and Cohen, J. D. 1999. Selective isolation of bacterial antagonists of Botrytis cinerea. Eur. J. Plant Pathol. 105: 95-101 https://doi.org/10.1023/A:1008641929417
  22. Pieterse, C. M. J., Van Wees, S. C. M., Hoffland, E., Van Pelt, J. A. and Van Loon, L. C. 1996. Systemic resistance in Arabidopsis induced by biocontrol bacteria is independent of salicylic acid accumulation and pathogenesis-related gene expression. Plant Cell 8: 1225-1237 https://doi.org/10.1105/tpc.8.8.1225
  23. Press, C. M., Wilson, M., Tuzun, S. and Kloepper, J. W. 1997. Salicylic acid produced by Serratia marcescens 90-166 is not the primary determinant of induced systemic resistance in cucumber or tobacco. Mol. Plant-Microbe Interact. 6: 761-768
  24. Raupach, G. S. and Kloepper, J. W. 2000. Biocontrol of cucumber diseases in the field by plant growth-promoting rhizobacteria with and without methyl bromide fumigation. Plant Dis. 84: 1073-1075 https://doi.org/10.1094/PDIS.2000.84.10.1073
  25. SAS Institute Inc. 1988. SAS/STAT Users Guide. Release 6.03 edn. SAS Institute, Cary, NC, U.S.A.
  26. Smith, K. P. and Goodman, R. M. 1999. Host variation for interactions with beneficial plant-associated microbes. Annu. Rev. Phytopathol. 37: 473-491 https://doi.org/10.1146/annurev.phyto.37.1.473
  27. Thompson, D. C. and Jenkins, S. F. 1985. Influence of cultivar resistance, initial disease, environment, and fungicide concentration and timing on anthracnose development and yield loss in pickling cucumbers. Phytopathology 75: 1422-1427 https://doi.org/10.1094/Phyto-75-1422
  28. Uri, N. D. 1998. Government policy and the development and use of biopesticides. Futures 30: 409-423 https://doi.org/10.1016/S0016-3287(98)00045-7
  29. Van Loon, L. C., Bakker, P. A. H. M. and Pieterse, C. M. J. 1998. Systemic resistance induced by rhizosphere bacteria. Annu. Rev. Phytopathol. 36: 453-483 https://doi.org/10.1146/annurev.phyto.36.1.453
  30. Van Wees, S. C. M., Pietcrse, C. M. J., Trijssenaar, A., Van't Westende, Y. A. M., Hartog, F. and Van Loon, L. C. 1997. Differential induction of systemic resistance in Arabidopsis by biocontrol bacteria. Mol. Plant-Microbe Interact. 6: 716-724
  31. Wasilwa, L. A., Correll, J. C., Morelock, T. E. and McNew, R. E. 1993. Reexamination of reces of the cucnrbit anthracnose pathogen Colletotrichum orbiculare. Phytopathology 83: 1190-1198 https://doi.org/10.1094/Phyto-83-1190

피인용 문헌

  1. vol.104, pp.8, 2014, https://doi.org/10.1094/PHYTO-11-13-0305-R
  2. strain GYL4 and its efficacy in the control of anthracnose vol.72, pp.8, 2016, https://doi.org/10.1002/ps.4181