Mycobiology

The Korean Society of Mycology (한국균학회)
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Mycoflora of Chicken-Viscera with Aid of RAPD Technique as a Tool for Confirmation

  • Gherbawy, Youssuf A. (Botany Dept., Faculty of Science, South Valley University) ;
  • Farghaly, Refaat M. (Food Hygiene Dept., Faculty of Veternary Medecine, South Valley University)
  • Published : 2002.03.31
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Abstract

A total of 100 samples of chicken-viscera were collected from different poultry-slaughtering houses in Austria;(20 samples of each of gizzard, heart, intestine, liver and spleen). Intestine and gizzard were heavy contaminated with moulds than other examined visceral organs($4.4{\times}10^5$ and $2.6{\times}10^4$ colonies/1g of the samples, respectively). Fungal contamination was not detected in all samples of heart and spleen. Eighty-five mould isolates were collected from the examined samples, the majority of isolates belonging to Aspergillus glaucus group(20.0%) and Trichoderma(14.1%). These isolates comprised 15 species belonging to 9 genera. Members of Aspergillus glaucus(telomorph: Eurotium) group and Trichoderma were further confirmed their identification using random amplified polymorphic DNA-polymerase chain reaction(RAPD-PCR) technique.

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References

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