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In vitro Evaluation of the Antifungal Activity of Propolis Extract on Cryptococcus neoformans and Candida albicans

  • Chee, Hee-Youn (Division of Biological Sciences, Medical School, Konyang University)
  • Published : 2002.06.30

Abstract

The antifungal activities of propolis on Cryptococcus neoformans and Candida albicans were evaluated. In microbroth culture assay, the MIC(minimum inhibitory concentration) of propolis for C. neoformans and C. albicans were 2 and 16 mg/ml, respectively. In propolis-included solid medium assay, the MIC of propolis for C. neoformans and C. albicans were 4 and 16 mg/ml, respectively. Propolis showed fungicidal activity against C. neoformans, whereas propolis possesed fungistatic activity against C. albicans. The MFC(minimum fungicidal concentration) for C. neoformans was 8 mg/ml. Cell morphology of C. neoformans was affected by treatment of propolis. In scanning electron microscope, the appearance of cell rupture was observed.

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References

  1. Bankova, V., Christov, R., Kujumgiev, A., Marcucci, M. C. and Popov, S. 1995. Chemical composition and antibacterial activity of Brazilian propolis, Z. Naturforsch. 50c: 167-172
  2. Christov, R., Bankova, V., Tsvetkova, I., Kujumgiev, A. and Delgado,T. A. 1999. Antibacterial furofuran Iignans from Canary Island propolis. Fitoterapia. 70: 89-92 https://doi.org/10.1016/S0367-326X(98)00044-6
  3. Hegazi, A. G., Abd El Hady, F. K. and Abd Allah, F. A. M. 2000. Chemical composition and antimicrobial activity of European propolis. Z. naturforsch. 55c: 70-75
  4. Hodema, E. and Kedzia, B. 1987. Investigation upon the combined action of propolis and antimycotic drugs on Candida albicans, Herba Pol. 33: 145-151
  5. Krell, R. 1996. Value-added products from bee keeping. Food and Agriculture Organization of the UN, Agriculture Service Bulletin 124
  6. Manolova, N. and Maksimova, V. 1987. Immuno-stimulating effect of propolis. Effect on cellular immunity. Acta-Microbiol-Bulg. 21: 76-81
  7. Matsuno, T., Jung, S.-K., Matsumoto, Y., Saito, M. and Morilawa, J. 1997. Preferential cytotoxicity to tumor cells of 3,5-diprenyl-4-hydroxycinnamic acid (artepillin C) isolated from propolis. Anticancer Res. 17: 3565-3568
  8. Ota, C., Unterkirche, C., Fantinato, V. and Shimizu, M. T. 2001. Antifungal activity of propolis on different species of Candida. Mycoses. 44: 375-378 https://doi.org/10.1046/j.1439-0507.2001.00671.x
  9. Tadaisi-Kikuni, N. B. and Schilcher, H. 1994. Electron microscopic and microcalorimetric investigations of the possible mechanism of the antibacterial action of a defined propolis provenance. Planta Med. 60: 222-227 https://doi.org/10.1055/s-2006-959463
  10. Thomsom, W. M. 1990. Propolis. Med. J. Aust. 153: 654

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  1. vol.2, pp.2, 2012, https://doi.org/10.1080/22311866.2012.10719118