Clinical and Experimental Reproductive Medicine

  • 제29권4호
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  • Pages.323-335
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  • 2002
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  • 2233-8233(pISSN)
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  • 2233-8241(eISSN)
대한생식의학회 (The Korean Society for Reproductive Medicine)
권호 표지

Laser Capture Microdissection을 이용한 유전자 발현 연구 (III) -생쥐 착상 부위 자궁 내강상피 조직에서 배아 병치 기간 동안 일어나는 유전자 발현에 관한 Microarray 분석-

Analysis of the Gene Expression by Laser Capture Microdissection (III) -Microarray Analysis of the Gene Expression at the Mouse Uterine Luminal Epithelium of the Implantation Sites during Apposition Period-

  • 윤세진 (차병원 여성의학연구소) ;
  • 전은현 (포천중문 의과대학교 생명과학 전문대학원) ;
  • 박창은 (차병원 여성의학연구소) ;
  • 고정재 (차병원 여성의학연구소) ;
  • 최동희 (차병원 여성의학연구소) ;
  • 차광열 (차병원 여성의학연구소) ;
  • 김세년 (디지탈 지로믹스) ;
  • 이경아 (차병원 여성의학연구소)
  • Yoon, Se-Jin (Infertility Medical Center, CHA General Hospital) ;
  • Jeon, Eun-Hyun (Graduate School of Life Science and Biotechnology, College of Medicine, Pochon CHA University) ;
  • Park, Chang-Eun (Infertility Medical Center, CHA General Hospital) ;
  • Ko, Jung-Jae (Infertility Medical Center, CHA General Hospital) ;
  • Choi, Dong-Hee (Infertility Medical Center, CHA General Hospital) ;
  • Cha, Kwang-Yul (Infertility Medical Center, CHA General Hospital) ;
  • Kim, Se-Nyun (Digital Genomics Inc.) ;
  • Lee, Kyung-Ah (Infertility Medical Center, CHA General Hospital)
  • 발행 : 2002.12.30
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초록

Object: The present study was accomplished to obtain a gene expression profile of the luminal epithelium during embryo apposition in comparison of implantation (1M) and interimplantation (INTER) sites. Material and Method: The mouse uterine luminal epithelium from IM and INTER sites were sampled on day 4.5 (Day of vaginal plug = day 0.5) by Laser Captured Microdissection (LCM). RNA was extracted from LCM captured epithelium, amplified, labeled and hybridized to microarrays. Results from microarray hybridization were analyzed by Significance Analysis of Microarrays (SAM) method. Differential expression of some genes was confirmed by LCM followed by RT-PCR. Results: Comparison of IM and INTER sites by SAM identified 73 genes most highly ranked at IM, while 13 genes at the INTER sites, within the estimated false discovery rate (FDR) of 0.163. Among 73 genes at IM, 20 were EST/unknown function, and the remain 53 were categorized to the structural, cell cycle, gene/protein expression, immune reaction, invasion, metabolism, oxidative stress, and signal transduction. Of the 24 structural genes, 14 were related especially to extracellular matrix and tissue remodeling. Meanwhile, among 13 genes up-regulated at INTER, 8 genes were EST/unknown function, and the rest 5 were related to metabolism, signal transduction, and gene/protein expression. Among these 58 (53+5) genes with known functions, 13 genes (22.4%) were related with $Ca^{2+}$ for their function. Conclusions: Results of the present study suggest that 1) active tissue remodeling is occurring at the IM sites during embryo apposition, 2) the INTER sites are relatively quiescent than IM sites, and 3) the $Ca^{2+}$ may be a crucial for apposition. Search for human homologue of those genes expressed in the mouse luminal epithelium during apposition will help to understand the implantation process and/or implantation failure in humans.

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