The Journal of Korean Medicine (대한한의학회지)

The Society of Korean Medicine (대한한의학회)
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The Effect of Kamihaengche-tang Plus Yukmijihwang-tangon Oxidant and Hg-induced Rabbit's Liver Cell Injury

가미행체탕 합 육미지황탕이 Oxidant 및 Hg에 의한 가토 간세포손상에 미치는 영향

  • 이수행 (동의대학교 한의과대학 비계내과학 교실) ;
  • 김원길 (동의대학교 한의과대학 비계내과학 교실) ;
  • 김우환 (동의대학교 한의과대학 비계내과학 교실)
  • Published : 2002.09.01
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Abstract

Objectives : This study was carried out to determine whether Kamihaengche-tang plus Yulanijihwang-tang (KCYH) exerts a protective effect against oxidant-induced liver cell injury. Methods : Cell injury was estimated by measuring lactate dehydrogenase (LDH) and alanine aminotransferase (ALT) release, and lipid peroxidation was estimated by measuring malondialdehyde, a product of lipid peroxidation in rabbit liver slices. Results : Oxidants (tBHP and $H_2O_2$) increased dose-dependently LDH release which was significantly prevented by 1% KCYH. The protective effect of KCYH against oxidant-induced cell injury was dose-dependent in the range of 0.05-1 % concentrations. Similarly, KCYH inhibited oxidant-induced lipid peroxidation in a dose-dependent manner. When liver tissues were exposed to Hg (0.5 mM), ALT activity in the medium and lipid peroxidation in tissues were markedly increased. These changes were prevented by 1% KCYH, KCYH restored toxicant-induced inhibition of cellular GSH content. KCYH increased the activities of catalase and glutathion peroxidase in oxidant-treated tissues. Conclusions : These results indicate that KCYH exerts a protective effect against oxidant-induced liver cell injury, and this effect is attributed to prevention of lipid peroxidation. These effects may be due to an increase in concentration of endogenous antioxidants.

Keywords

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