Journal of Bio-Environment Control (생물환경조절학회지)

The Korean Society for Bio-Environment Control (한국생물환경조절학회)
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Autotrophic Growth of Limonium spp. 'Ocean Blue' Plantlets In Vitro as Affected by PPF, NAEH and $\textrm{CO}_2$ Concentration

스타티스 ‘오션 블루’의 자가영양배양시 광도, 환기횟수 및 $\textrm{CO}_2$ 농도가 소식물체의 기내 생육에 미치는 영향

  • 정기원 (경상대학교 응용생명과학부) ;
  • 정병룡 (경상대학교 농업생명과학연구원)
  • Published : 2002.09.01
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Abstract

Growth and development of Limonium spp.‘Ocean Blue’plantlets were studied under three levels of photosynthetic photon flux (PPF),70,150 and 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ , two levels of $CO_2$ concentration, 500 and 1000 $\mu$mol. $m^{-1}$ , and two levels of number of air exchanges per hour (NAEH),0.1 $h^{-1}$ and 2.8 $h^{-l}$. Explants were obtained from photomixotrophically-micropropagated plantlets. Four explants per vessel were cultured under cool-white fluorescent lamps for 16 h. $d^{-1}$ at 25$\pm$11$^{\circ}C$ and 70~80% relative humidity. In treatments of 2.8 $h^{-1}$ NAEH, a 10 mm round hole made on the vessel cap was sealed with a microporous filter and two $CO_2$ concentrations in the culture rooms were provided from a liquefied $CO_2$ tank. Fresh and dry weights, height, length of the longest root, number of loaves, and leaf area significantly increased with increasing PPF and especially, $CO_2$ concentration. Growth was enhanced by a 2.8 $h^{-1}$ NAEH. Overall, treatment with a 220 $\mu$mol. $m^{-2}$ . $s^{-1}$ PPF and a 1000 $\mu$mol. $m^{-1}$ $CO_2$ resulted in the most vigorous growth of Limonium spp. ‘Ocean Blue’ plantlets.s.

자가영양배양시 광도,환기횟수 및 $CO_2$농도가 스타티스 ‘Ocean Blue’ 소식물체의 생장에 미치는 영향을 조사하였다 MS 배지에서 광흔합영양해양을 통하여 증식된 스타티스 식물체에서 2~3장의 잎을 가진 줄기 절편체를 채취하여 배양용기당 4개씩 치상하였다. 절편체는 온도 25$\pm$1$^{\circ}C$. 상대습도 70~ 80%인 배양실에서 cool-white 형광램프로 명기동안 70, 150, 또는 220$\mu$mol. $m^{-2}$ . $s^{-1}$의 광을 공급하면서 41일 동안 배양하였다. MS 기본배지를 사용하였으며, sucrose와 vitamin은 첨가하지 않았다. 광도가 높아질수록 생체 중,건물중 및 엽면적이 증가하였다. $CO_2$농도를 높여준 경우 초장, 생체중, 건물중 및 엽면적이 증가하였다. 뿌리길이와 엽록소 농도는 광도나 $CO_2$의 영향을 받지 않았다. 배양용기 내의 환기횟수를 높이고, 고광도의 $CO_2$를 공급할수록,그리고 PPF를 증가시킬수록 스타티스 소식물체의 자가영양배양에서의 생육이 촉진되었다.

Keywords

References

  1. Coumac, L., I. Cirier, and P. Chagvardieff. 1992. Improvement of photoautotrophic Solanum tuberosum plantlet culture by light and $CO_2$: Differential develop-ment of photosynthetic characteristics and varietal con-straints. Acta Hort. 319:53-58
  2. Desjardins, Y., A. Gosselin, and S. Yelle. 1987. Accli-matization of in vitro strawberry plantlets in $CO_2$enriched environments and supplemental lighting. J. Amer. Soc. Hort. Sci. 112:846-851
  3. Desjardins, Y., F. Laforge, C. Lussier, and A. Gosse-lin. 1988. Effect of $CO_2$ enrichment and high photo-synthetic photon flux on the development of autotro-phy and growth of tissue cultured strawberry, rasp-berry and asparagus plants. Acta Hort. 230:45-53
  4. Desjardins, Y., A. Gosselin, and M. Lamarre. 1990. Growth of transplants and in vitro-cultured clones of asparagus in response to $CO_2$ enrichment and supple-mental lighting. J. Amer. Soc. Hort. Sci. 115:364-368
  5. Fujiwara, K., T. Kozai, and I. Watanabe. 1987. Funda-mental studies on environments in plant tissue culture vessels. J. Agr. Meteorol. 43:21-30 https://doi.org/10.2480/agrmet.43.21
  6. Fujiwara, K., S. Kira, and T. Kozai. 1992. Time course of $CO_2$ exchange of potato cultures in vitro with differ-ent sucrose concentrations in the culture medium. J. Agr. Meteorol. 48:49-56 https://doi.org/10.2480/agrmet.48.49
  7. Grout, B.W. and S. Millam. 1985. Photosynthetic development of micropropagated strawberry plantlets following transplanting. Ann. Bot. 55:129-131 https://doi.org/10.1093/oxfordjournals.aob.a086872
  8. Jeong, B.R., K. Fujiwara, and T. Kozai. 1995. Carbon dioxide enrichment in autotrophic micropropagation:Methods and Advantages. HortTechnology 3:332-334
  9. 정병룡, 이은주, 김수정,황승재. 1996. 발근단계에서의 기내 영양체계와 기외 순화방법이 카네이션과 딸기 배양묘의 생육에 미치는 영향. 한국식물조직배양학회 발표요지. pp. 37-38
  10. Kim, J.S. and B.R. Jeong. 2001. Growth, Photosyn-thetic rate and stomatal function of Spathiphyllum in vitro as affected by PPF and NAEH. J. Kor. Hort. Sci. 42:351-352
  11. Kirdmanee, C., Y. Kitaya, and T. Kozai. 1995a. Effects of $CO_2$ enrichment and supporting material in vitro onphotoautotrophic growth of Eucalyptus plantlets in vitro and ex vitro: Anatomical comparisons. Acta Hort. 393:111-118
  12. Kirdmanee, C., Y. Kitaya, and T. Kozai. 1995b. Effects of $CO_2$ enrichment and supporting material on growth,photosynthesis and water potential of Euclyptus shoots/plantlets cultured photoautotrophically in vitro. Environ Control Biol. 33:133-141 https://doi.org/10.2525/ecb1963.33.133
  13. Kozai, T. and Y. Iwanami. 1988. Effects of $CO_2$ enrich-ment and sucrose concentration under high photon fluxes on plantlet growth of carnation (Dianthus caryophylIus L.) in tissue culture during the propaga-tion stage. J. Japan. Soc. Hort. Sci. 57:279-288 https://doi.org/10.2503/jjshs.57.279
  14. Kozai, T. and K. Sekimoto, 1988. Effects of the num-ber of air changes per hour of the closed vessel and the photosynthetic photon flux on the carbon dioxide con-centration inside the vessel and the growth of straw-berry plantlets in vitro. Environ. Control Biol. 26:21-29 https://doi.org/10.2525/ecb1963.26.21
  15. Kozai, T., S. Kushihashi, C. Kubota, and K. Fujiwara, 1992a. Effect of the difference in air temperature between photoperiod and dark period on morphogene-sis and growth of potato plantlets in vitro under photo-autotrophic and $CO_2$ enriched conditions. J. Japan. oc. Hort. Sci. 61:93-98 https://doi.org/10.2503/jjshs.61.93
  16. Kozai, T., K. Fujiwara, M. Hauashi, and J. Aitken-Christe, 1992b. The in vitro environment and its Con-trol in micropropagation. pp. 247-282. In: Transplant Production System (K. Kurata and T. Kozai, Eds). Kluwer Academic Publishers
  17. Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  18. 이용범, 이병일. 1994. $CO_2$ 장기 시용이 토마토의엽온, 확산저항 및 광합성에 미치는 영향. J. Kor. Soc. Hort. Sci. 35:421-428
  19. 이은주. 1998. Study of the Autotrophic Culture of Limomnium spp. cv. Misty Blue. 경상대학교. 석사학위논문. p. 26-27