Filter- Photometry of Chemiluminescence from Firefly Luciferin Intermediate M420 in Deoxygenated Dimethyl Sulfoxide

  • Shibata, Rikuo (Department of Applied Chemistry, Faculty of Engineering, Toyo University) ;
  • Yoshida, Yasuhiko (Department of Applied Chemistry, Faculty of Engineering, Toyo University) ;
  • Wada, Naohisa (Department of Life Science, Faculty of Life Science, Toyo University)
  • Published : 2002.08.01

Abstract

The intermediate M420 formed in a solution of firefly luciferin in deoxygenated dimethyl-sulfoxide added potassium f-butoxide was observed to emit yellow-green and red light by filter-photometry. By H-NMR, M420 was found to be deprotonated at the site where luciferin reacts with oxygen.

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