YAKHAK HOEJI (약학회지)

The Pharmaceutical Society of Korea (대한약학회)
권호 표지

Flow Cytometrical Investigation on in vitro Immunomodulating Activity of PJ-4, a Protein-polysaccharide from Culture Flitrate of Insects-born Fungus Paecilomyces Tenuipes DGUM 32001

눈꽃동충하초 (Paecilomyces tenipes DGUM 32001) 균사배양물로부터 분리한 단백다당체 PJ-4의 in vitro 면역활성

  • 정경수 (충남대학교 약학대학) ;
  • 이지선 (충남대학교 약학대학) ;
  • 김용해 (신화제약 중앙연구소) ;
  • 한영환 (동국대학교 자연과학대학 생물학과) ;
  • 이만형 (대구가톨릭대학교 약학대학)
  • Published : 2002.06.01
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Abstract

In the previous report, we described the in vivo antitumor activity of PJ-4, a protein-polysaccharide fraction prepared from the culture filtrate of an insect-born fungus, Paecilomyces tenuipes DGUM 32001. In the present study, we elucidated the immunomodulating activity of PJ-4 on the BALB/c mouse splenic lymphocytes using flow cytometrical techniques. As a result, PJ-4 was found to stimulate the lymphocytes not only to form lymphoblasts but also to express CD25 (IL-2 receptor $\alpha$ chain) molecule, which is well known as a T cell activation marker. More interestingly, its T cell stimulatory activity was more strongly exerted on CD8$^{+}$ T cells than on CD4$^{+}$ T cells. All these data suggest that PJ-4 exerts its antitumor activity at least partly through stimulation of T cells which play major roles in the cell-mediated immune system.tem.

Keywords

References

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