약학회지 (YAKHAK HOEJI)

  • 제46권3호
  • /
  • Pages.197-202
  • /
  • 2002
  • /
  • 0377-9556(pISSN)
  • /
  • 2383-9457(eISSN)
대한약학회 (The Pharmaceutical Society of Korea)
권호 표지

Macrophage의 IL-1$\beta$Gene Expression 대한 Lectin-conjugated Ellagitannin의 효과

Effects of Lectin-conjugated Ellagitannin on the IL-1$\beta$, Gene Expression of Macrophage

  • 발행 : 2002.06.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Lectin-conjugated praecoxin A, which has developed as a missile antitumor drug, is the one that has conjugated with wheat germ agglutinin (WGA), a kind of carbohydrate-binding protein (lectin) especially bound to melanoma. Praecoxin A is a kind of tannin extracted and purified from plants. Beside this direct antitumor effect as tannins, we have examined an activation of macrophage by lectin-conjugated praecoxin A. We also confirmed the gene expression of IL-1 $\beta$, both in vitro and in vivo. We added 1, 10, 100 $\mu\textrm{g}$/mι of lectin-conjugated praecoxin A, 10 $\mu\textrm{g}$/mι of lectin, 10 $\mu\textrm{g}$/mι of praecoxin A to normal murine macrophage and analyzed the extracted total RNAs by RT-PCR after 4, 8, 12, 24 hours. Our data demonstrated that lectin-conjugated praecoxin A increased IL-1$\beta$, mRNA expression in a dose dependent manner. However, the effectiveness of lectin-conjugated praecoxin A was not superior to lectin and praecoxin A.

키워드

참고문헌

  1. Kuby, J : Cell-Mediated Immunity. J. Kuby. Immunology 4th edited. 347 (2000)
  2. Stites, D. P. and Terr, A. I. : Basic and Clinical Immunology. 7th ed. 870 (1991)
  3. Adams, D. O. and Hamilton, T. A.: The cell biololgy of macrophage activation. Annu. Rev. Immunol. 2, 283 (1984) https://doi.org/10.1146/annurev.iy.02.040184.001435
  4. Roitt, I., Brostoff, J. and Male D. Immunology. 2nd ed. 13, 25 (1989)
  5. Banchereau, J. et al. : The CD40 antigen and its ligand. Ann. Rev. Immunol. 12, 881 (1994) https://doi.org/10.1146/annurev.iy.12.040194.004313
  6. Brodsky, F. M. and Guagliardi, L. : The cell biology of antigen processing and presentation. Ann. Rev. Immunol. 9, 707 (1994)
  7. Chantry, D. and Feldmann, M. : The role of cytokines in autoimmunity. Biotechnol. Ther. 1, 361 (1991)
  8. Clark, E. A. and Ledbetter, J. A. : How Band T cells talk to each other. Nature. 367, 425 (1994) https://doi.org/10.1038/367425a0
  9. Parker, D. C. : T cell-dependent B cell activation. Ann. Rev. Immunol. 11, 331 (1993)
  10. Romagnani, S. : Cytokine production by human T cells in disease states. Ann. Rev. Immunol. 12, 227 (1994) https://doi.org/10.1146/annurev.iy.12.040194.001303
  11. Ullman, K., Northrop, J. P., Veirweij, C. L. and Crabtree, G. R : Transmission of signals from T lymphocyte antigen receptor to the gene responsible for cell proliferation and immune fuction. Ann. Rev. Immunol. 8, 421 (1989) https://doi.org/10.1146/annurev.iy.08.040190.002225
  12. Abbas, A. K, Lichtman, A. H. and Pober, J. S. Cellular and Molecular Immunology. 1st ed. (1991)
  13. Dinarello, C. A. : Role of interleukin-1 in infectious diseases. Immunol. Rev. 127, 119 (1992) https://doi.org/10.1111/j.1600-065X.1992.tb01411.x
  14. Arai, K., Lee, F., Miyajima, A., Arai, N. and Yokoda T. : Cytokines; coordinatiors ofimmune and inflammatory responses. Annu. Rev. Biochem. 59, 783 (1990) https://doi.org/10.1146/annurev.bi.59.070190.004031
  15. Balkwill, F. R., Burke, F. : The cytokine network. Immunol. Today. 10, 299 (1989) https://doi.org/10.1016/0167-5699(89)90085-6
  16. Lee, M. W., Tanaka, T., Nonaka, G. and Nishioka, I. : Hirsunin, an ellagitannin with diarylheptanoid moiety, from Alnus hirsuta var. microphylla. Phytochemistry. 31, 967 (1992)
  17. Chang, J. H., Cho, J. H., Kim, H. H., Lee, M. W., Han, S. S. and Lee, D. I. : Anti-tumor Activity of Pedunculagin, One of the Ellagitannin. Arch. Pharm. Res. 18, 396 (1995) https://doi.org/10.1007/BF02976342
  18. 赤木忠厚, 乾直道외 20. 組織培養の技術. 19 (1982)
  19. 瀨野得二, 小山秀機, 黑木登志夫. Manual of Selected Culture Cell lines for Bioscience and Biotechnology. 132 (1993)
  20. Coligan, J. E., Kruisbeek, A. M., Margulies, D. H., Shevach, E. M. and Strober; W. Current Protocols in Immunology. wiley Interscience. 4, 2. 1 (1996)
  21. Kim, H. K., Han, K. S. and Lee, D. I. : Effects of Lectinconjugated Ellagitannin on Antitumor Activity. Yakhak Hoeji. 44, 6, 607 (2000)
  22. Kim, H. K., Han, K. S. and Lee, D. I. : Effects of Lectinconjugated Ellagitannin on Inhibiton of Melanoma Metastasis. Yakhak Hoeji. 44, 6, 601 (2000)