Journal of Food Hygiene and Safety (한국식품위생안전성학회지)

The Korean Society of Food Hygiene and Safety (한국식품위생안전성학회)
권호 표지

In vitro Screening of Natural Product Inhibitors on the UVB Phototoxicity

In vitro 실험법에 의한 천연물 중의 UVB 광독성 억제제 검색

  • 김현진 (한국화학연구원 부설 안전성평가연구소) ;
  • 이경아 (충남대학교 약학대학) ;
  • 김봉희 (충남대학교 약학대학)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The phototoxicity inhibitory activity of 15 natural products having antiinflammatory effect was screened by three in vitro methods : yeast growth inhibition test with Candida albicans, RBC photohemolysis and MTT assay. We induced phototoxic reaction by irradiating UVB (312 nm) on chlorpromazine (CPZ) that has been widely documented as phototoxic agent in clinical and experimental studies and then observed the effects of the natural products after treating them with CPZ. In yeast growth Inhibition test, P. persica and E. officinalis showed the inhibitory effect on the UVB phototoxicity and E. officinalis, yeast, P. suffruticosa showed phototoxicity inhibitory effect in that their ％ hemolysis compared with control were 45.76 $\pm$ 0.91, 34.42$\pm$1.01, 35.30 $\pm$4.76 on UVB. In MTT assay, all tested natural products increased cell viability compared with the contort.

소염효과를 나타내는 생약 15종을 선별하여 이들 생약 추출물이 Chloropromazine에 의한 UVB 광독성에 미치는 영향을 in vitro 실험법 중 Candida albicans test, RBC photohemolysis, MTT assay에 의한 cytotoxicity 측정법의 방법을 이용하여 조사하였다. Candidal albicans teat에 의해 광독성의 광량 및 화학물질 의존성을 관찰할 수 있었으며, 광독성 억제 효과를 검색하기에 적당한 UVB조사량 및 CPZ농도는 2.1 J/$\textrm{cm}^2$, 0.6mg인 것으로 생각되었다. Candida albicans test에 의해 CPZ에 의한 UVB 광독성에는 P. persica, E. officinalis가 억제효과를 보임을 알 수 있었으며, RBC photohemolysis 결과 모든 생약시료들에 대해 hemolysis가 감소되었으며, 특히, Yeast, P. suffruticosa에 의한 % hemolysis는 34.42$\pm$1.01, 35.30$\pm$4.7로 유의성 있는 억제효과를 나타냄을 관찰할 수 있었다. MTT assay에 의한 cytotoxicity 측정 결과, UVB 조사에 이하여 세포생존율은 감소되었고, 시료처리시 세포생존율은 증가됨을 관찰할 수 있었다.

Keywords

References

  1. Seckmeyer, G. B., Mayer, R. E. and Bernhard, G.: UV-B in Germany Higher in 1993 than in 1992. Geography, Res. Lett. 21, 577 (1994) https://doi.org/10.1029/94GL00567
  2. Augustin C, et aI: Use of dermal equivalent and skin equivalent models for identifying phototoxic compounds in vitro, Photoderm Photoimmun & Photomed, 13, 27-36 (1997) https://doi.org/10.1111/j.1600-0781.1997.tb00105.x
  3. 조희구, 김해경; 이규태: 서울의 오존전량 변화 경향. 한국기상학회지 30(2), 219 (1994)
  4. Shin, et al.: Estimating skin cancer caused by UVB. Kor. J. ENVIRON. TOXICOL. 11, 1-10 (1996)
  5. Edward, S. M., et at.'. Quantitative m vitro assessment of phototoxicity using a human skin model, $skin^{2TM}$, Photoderm Photoimmun & Photomed, 10, 111-117 (1994)
  6. Kerr, J. B. and Wardle, D. I.: Ozone and UV-B Monitoring Program in Canada in Private Communication. (1994)
  7. Woodcock, A. and Magnus, I. A. : The sunburn cell in mouse skin: preliminary quantitative studies on its production. Br. J. Dermatol 95, 459-468 (1979) https://doi.org/10.1111/j.1365-2133.1976.tb00854.x
  8. Donald, L. B., Daniel, P. H. and Thomas, V. O.: An animal model of solar aged skin : Histological, Physical, and visible changes in UV irradiated hairless mouse skin. Photochem.PhotobioI., 46(3), 367-378 (1987) https://doi.org/10.1111/j.1751-1097.1987.tb04783.x
  9. Gensler, H. L., Gerrish, K. E., Williams, T., and Roe, G.:Provontation of photocarcinogensis and UV-induce immuno-suppression in mice by topical tannic acid. Nutr. Cancer., 22,121 (1994) https://doi.org/10.1080/01635589409514337
  10. Halliwell, B. and Gutteridge, J.: M. C. Arch. Biochem. Biophys., 280, 1 (1990) https://doi.org/10.1016/0003-9861(90)90510-6
  11. Keino, H., M. S, N. H., B. T.,and K. S.: Protection by Lascorbic acid against phototoxicity in tm-protoporphyrintreated suckling rats. Biol Neonate, 63(2), 183-190 (1993) https://doi.org/10.1159/000243930
  12. Klingman, A. M. and Kaidbey, K. H.: Phototoxicity to benozaprofen, Eur. J. Rheum. Inflam., 5, 123-137 (1982)
  13. Ljunggren, B.: Propionic acid-derived non-steroidal antiinflammatory drugs are phototoxic in vitro, Photodermatology, 2, 3-9 (1985)
  14. Raymond J. M. Niesink, John de Vries, and Mannfred A. Hollnger: Toxicology Principles and Applications, CRC Press 512-514 (1996)
  15. K-ahn. G., Fleischaker. B.: Red blood cell hemolysis by photosensitizing compounds. J. Inverst. Dermatol. 56, 85-90 (1971) https://doi.org/10.1111/1523-1747.ep12260639
  16. Ljinggern, B., Moller, H.: Phototoxic reaction to Chlorpromazine as studied with the quantitative mouse tail technique. Acta. Derum., 56, 373-376 (1976)
  17. Saucin, M.: Mechanism of photosensitization by Phenothiazine derivatives. Acta. Int. Physiol. Biochem., 87, 1051-1052 (1979)
  18. Guy, F. W., Kays, H. K., and Alvert, M. K. : Phototoxicity from benoxaprofen; In vivo and in vitro studies. Photochem. Photobiol., 36, 59-64 (1982) https://doi.org/10.1111/j.1751-1097.1982.tb04340.x
  19. Moore, D. E. and Tamat, S. R. : Photosensitization by drugs. J. Pharm. Pharmacol., 32, 172-177 (1989)
  20. Lijunggem, B., Moller, H.: Phenothiazine phototoxicity; An experimental study on chlorpromazine and its metabolites. J. Invest. Dermatol., 68, 313-317 (1977) https://doi.org/10.1111/1523-1747.ep12494582
  21. Chignell. C. F, Motten. A. G. and Buettner. G. R.: Photoinduced free radicals from chlorpromazine and related Phenothiazines : relationship to phenothiazine induced Photosensitization. Environ. Health. Perspect. 64, 103-110 (1985) https://doi.org/10.2307/3430002
  22. Marko, J., Vermeersch, G., Febvay-Garot, N., and LablacheCombier, A.: Photo-CIDNP in nuclei acid bases and their nucleotides induced by chlorpromazine and analogs. Photochem. Photobiol., 42, 213-221 (1985) https://doi.org/10.1111/j.1751-1097.1985.tb08934.x
  23. Tomas, A. C., Gary, A. E., and Irene, E. K.: Photoaddition of chlorpromazine to guanosine-5-monophosphate. Photochem. Photobial., 43, 607-613 (1986) https://doi.org/10.1111/j.1751-1097.1986.tb05635.x