한국환경성돌연변이발암원학회지 (Environmental Mutagens and Carcinogens)
- 제22권3호
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- Pages.175-182
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- 2002
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- 1225-6307(pISSN)
Human Estrogen Receptor Ligand Binding Domain (hER LBD)과 Co-activator로 구성된 효모 Two-Hybrid System을 이용한 내분비계장애물질 검출계의 구축
Construction of the Detection System of Endocrine Disrupters using Yeast Two-Hybrid System with Human Estrogen Receptor ligand Binding Domain and Co-activators
초록
Endocrine disruptors (EDs) are the chemicals that affect endocrine systems through activation or inhibition of steroid hormone response. It is necessary to have a good system to evaluate rapidly and accurately endocrine-disrupting activities of suspected chemicals and their degradation products. The key targets of EDs are nuclear hormone receptors, which bind to steroid hormones and regulate their gene transcription. We constructed a co-expression system of Gal4p DNA binding domain (DBD)- ligand binding domain of human estrogen receptor
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