Toxicological Research

Korean Society of Toxicology & Korea Environmental Mutagen Society (한국독성학회)
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Genotoxicity Studies of DA-6034, a New Flavonoid Derivative

새로운 플라보노이드 유도체인 DA-6034에 대한 유전독성에 관한 연구

  • 강병철 (서울대학교병원 임상의학연구소) ;
  • 권은아 (서울대학교병원 임상의학연구소) ;
  • 이나래 (서울대학교병원 임상의학연구소) ;
  • 안병옥 (동아제약 (주) 연구소) ;
  • 김원배 (동아제약 (주) 연구소) ;
  • 이상구 (서울대학교병원 임상의학연구소) ;
  • 이국현 (서울대학교병원 임상의학연구소) ;
  • 정진호 (서울대학교병원 임상의학연구소) ;
  • 성명훈 (서울대학교병원 임상의학연구소)
  • Published : 2002.12.01
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Abstract

Inflammatory bowel disease (IBD) is a multifactorial disorder with unknown etiology and pathogenesis. Eupatilin, a kind of flavonoids, has been known to be effective for chronic diarrhea in Korea. In this study, we have investigated the genotoxicity of DA-6034, a new synthetic derivative of Eupatilin, wing in vitro and in viuo system such as Ames reverse mutation test, chromosomal aberration test and micronucleus test. in Ames reverse mutation test, DA-6034 treatment at the dose range up to 5,000 $\mu\textrm{g}$/ plate did not induced mutagenicity in Salmonella typhimurium TA98, TA100, TA102, TA1535, TA1537 with and without metabolic activation. Any significant aberration wasn't observed in chinese hamster lung(CHL) fibroblast cells treated with DA-6034 at the concentration of 5, 2.5, 1.25 mg/ml both in the absence and presence of metabolic activation system. In mouse micronucleus test, no significant increase in the occurrence of micronucleated polychromatic erythrocytes was observed in ICR male mice orally administered with DA-6034 of the doses of 2.0, 1.0, 0.5 g/kg. These results indicate that DA-6034 has no mutagenic potential under the condition in this study.

Keywords

References

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