Journal of Plant Biotechnology

  • 제29권1호
  • /
  • Pages.31-36
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  • 2002
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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품종별 대두 〔Glycine max L.〕 자엽에서의 부정근 형성

Adventitious Root Formation from Cotyledon in Soybean (Glycine max L.) Cultivars

  • Ha, Keon-Soo (Kangwon Province Agricultural Research & Extension Services) ;
  • Han, Tae-Jin (Division of Life science, Hallym University)
  • 발행 : 2002.03.01
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초록

대두의 자엽 절편을 이용한 부정근 형성과 품종 반응을 비교하였다. 품종별 부정근 형성 양상은 직접적인 부정근 형성 품종과 callus형성이 동시에 이루어지는 품종들로 구분되었으며, callus 형성이 많은 품종에서는 부정근 형성수가 적었다. 자엽 절편에서의 부정근 형성은 절편의 향축면 치상시에만 부정근이 형성되었고, 배축면과 향축면이 동시에 치상될 경우에도 향축면에서만 부정근이 형성되며, 절편의 배축이 존재하였던 방향에서만 부정근이 형성되는 방향성이 존재하였다. 부정근 형성시 자엽 절편 내 탄수화물은 형성초기보다 형성이 가장 많은 유기 4일에 가장 많이 관찰되었으며 ,형성된 부정근의 생장이 이루어지는 7일 이후에는 관찰되지 않았다.

The patterns of adventitious root formation from cotyledons for each cultivar of soybeans were compared. The results of adventitious root formation in cultivars are classified as two groups; the first group showed the direct adventitious root formation, and the second group resulted in the callus and adventitious root formation. The cultivars that have much callus formation had less the adventitious root formation. The adventitious root formation in the cotyledonary explants was occured only at the inoculation of adaxial side. When adaxial and abaxial side was inoculated simultaneously, the adventitious roots were formed at the adaxial side. Thus, it suggests that there must be direction to some extent. Starch in the cotyledonary explants were more abundant at the 4 days after induction than at the early stage of the adventitious root formation, but the starch was not observed after 7 days, that the growth stage of adventitious roots.

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