Journal of Plant Biotechnology

  • 제29권1호
  • /
  • Pages.19-23
  • /
  • 2002
  • /
  • 1229-2818(pISSN)
  • /
  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지
DOI QR코드

DOI QR Code

신초 Cluster 형성에 의한 Lilium Asiatic Hybrid 'Hae Hwa'의 기내번식

In Vitro Propagation of Lilium Asiatic Hybrid 'Hae Hwa' via the Formation of Shoot Clusters

  • 발행 : 2002.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Lilium asiatic hybrid 'Hae Hwa'의 인편 및 자구절편 배양으로 신초 cluster를 유도하여 자구를 대량증식하고, 형성된 신초 cluster에서 정상적인 자구를 대량생산하고자 일련의 실험을 실시하였다. MS배지에 BA 1.0mg/L와 IAA 0.5 mg/L가 첨가된 배지에서 신초 cluster의 유도는 인편보다 자구절편을 배양하는 것이 효과적이었다. MS배지에 BA 5.0 mg/L와 IAA 0.5 mg/L 첨가배지에서 신초수 및 신초무게가 양호하여 형성된 신초 cluster 절편에서 신초 cluster의 증식에 적합하였다. 또한 자구의 비대는 MS 배지에 sucrose 60∼90 g/L가 첨가된 배지에서 양호하였다. MS배지에 sucrose 30∼90 g/L를 주입한 5L airlift 생물반응기에 신초 cluster 절편체를 배양한 결과, 신초만 무성하게 자랐고 자구비대는 불량하였다. 신초 cluster를 8주간 배양한 후에 2배 MS 염류와 sucrose 250g/L, 활성탄 5 g/L를 포함한 액체배지를 동일용기에 첨가하여 배양한 결과, 명배양에서 형성된 자구수는 증가하였으나 소자구의 생장은 명, 암 모두 비슷하였다. 이로써 형성된 소자구의 무게가 53∼68 mg이며, BA 5.0 mg/L와 IAA 0.5 mg/L가 첨가된 MS 배지에서 신초 cluster를 증식한 후, 액체배지 첨가방법에 의하여 효율적인 소자구의 생산이 이루어졌다.

This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid 'Hae Hwa'. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.

키워드

참고문헌

  1. Han BH (2001) Establishment of suitable media in bioreactors for micropropagation of Lilium spp. In: Micropropagation of lily bulblets in bioreactor by using bulblets with swollen basal plates and industrialization of lily bulb production for exporting. (The third years' report in G7 project) pp 29-108
  2. Han BH, Yae BH, Goo DH, Ko JY (1999a) Effects of growth regulators and light on the formation and proliferation of bulblets with swollen basal plate from in vitro culture of bulbscales in Lilium oriental hybrid 'Casa Blanca'. J. Kor. Soc. Hort. Sci. 40:463-466
  3. Han BH, Yae BW, Goo DH, Ko JY (1999b) The formation and growth of bulblets from bulblet sections with swollen basal plate in Lilium Oriental Hybrid 'Casa Blanca'. J. Kor. Soc. Hort. Sci. 40:747-750
  4. Han BH, Yae BW, Goo DH, Ko JY (1999c) Effect of inorganic salts in MS medium, sucrose, and activated charcoal on bulblet formation from in vitro bulbscales in Lilium Oriental Hybrid 'Casa Blanca'. Kor J Plant Tiss Cult 26:103-107
  5. Han BH, Yae BW, Goo DH (2001) Micropropagation of Lilium oriental hybrid 'Casa Blanca' using bulblet sections with swollen basal plate in bioreactor. Kor. J. of Plant Tissue Cult 28:135-140
  6. Leshem B, Lilien-Kipnis H, Steinitz B (1982) The effect of light and of explant orientation on the regeneration and subsequent growth of bulblets on Lilium Iongiflorum Thunb. bulb-scale sections cultured in vitro. Sci Hort 17:129-136 https://doi.org/10.1016/0304-4238(82)90005-X
  7. Maesato K, Sharada K, Fukui H, Hara T, Sarma KS (1994) In vitro bulblet regeneration from bulbscale explants of Lilium japonicum Thunb. Effect of plant growth regulators and culture environment. J. Hort. Sci. 69:289-297
  8. Murashige T, Skoog F (1962) A revised medium for rapid growth and biossays with tobacco tissue culture. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  9. Nhut DT (1998) Micropropagation of lily (Lilium Iongiflorum) via in vitro stem node and pseudo-bulblet culture. Plant Cell Rep 17:913-916 https://doi.org/10.1007/s002990050508
  10. Nhut DT, Le BV, Fukai S, Tanaka M, Van Thanh KT (2001) Effects of activated charcoal, explant size, explant position and sucrose concentration on plant and shoot regeneration of Lilium longiflorum via young stem culture. Plant Growth Regulation 33:59-65 https://doi.org/10.1023/A:1010701024963
  11. Niimi Y (1984) Effect of $\alpha$-naphthalenacetic acid and 6-benzylaminopurine on the development of excised-bulbs (LiIium rubellum Baker) cultured in vitro both in diffused light and in continuous darkness, and the leaf emergence from the bulbs in vivo. J Jap Soc Hort Sci 53:59-65 https://doi.org/10.2503/jjshs.53.59
  12. Niimi Y (1985) Factors affecting the regeneration of growth of bulblets in bulb scale cultures on Lilium rubrIlum Barker. J Jap Soc Hort Sci 4:82-86 https://doi.org/10.2503/jjshs.54.82
  13. Novak FJ, Petru E (1981) Tissue culture propagation of Lilium hybrids. Scientia Hort 14:191-199 https://doi.org/10.1016/0304-4238(81)90012-1
  14. Stimart DP, Ascher PD (1981) Developmental responses of Lilium Iongiflorum bulblets to constant or alternating temperatures in vitro. J Amer Soc Hort Sci 106:450-454
  15. Takayama S, Misawa M (1979) Differentiation in Lilium bulbscales grown in vitro. Effect of various cultural conditions. Physiol Plant 46:184-190 https://doi.org/10.1111/j.1399-3054.1979.tb06555.x
  16. Van Aartrijk J, Blom-Barnhoon GJ (1981) Growth regulator requirements for adventitious regeneration from LiIium bulb-scale tissue in vitro, in relation to duration of bulb storage and cultivar. Scientia Hort 14:261-268 https://doi.org/10.1016/0304-4238(81)90021-2