Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
권호 표지

Introduction of Hog Cholera Virus Gene into Potato Plants by Agrobacterium-mediated Transformation and the Analysis of Its Expression

  • Kim, Hyun-Soon (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Jeon, Jae-Heung (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology) ;
  • Kim, Cheol-Jung (Lab. of Infectious Disease, Disease of Veterinary Medicine, Chungnam National University) ;
  • Hyouk Joung (Plant Cell Biotechnology Lab., Korea Research Institute of Bioscience and Biotechnology)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The HCV gene was expressed in potato plants under the control of the constitutive CaMV 355 promoter or tuber-specific patatin promoter. Solanum tuberosum plants carrying a plant expression vector harboring the encoding region of HCV gene were generated by Agrobacterium tumefaciens-mediated in vitro transformation methods. The presence of HCV gene in the plant genome was detected by PCR and DNA hybridization experiments. We obtained the 5 lines of transgenic potato with the pMBPHCV construct and 4 lines of transgenic potato with the pATHCV construct. The HCV transgenic stably integrated into the potato genome, as well as their transcription. HCV mRNA was identified in leaf and tuber tissues of transgenic plants by Northern blot analysis. The transgenic potato plants produced the expected transcript, and the corresponding HCV protein accumulated in individual transgenic plants.

Keywords

References

  1. Current Opinion in Biotechnology v.11 Plants for delivery of edible vaccine. Amanda MW;Arntzen CJ https://doi.org/10.1016/S0958-1669(00)00070-7
  2. Transfenic Research v.6 Expression of cholera toxin B subunit oligomers in transgenic potato plants Arakawa T;Chong DKX;Merritt JL;Langhridge WHR https://doi.org/10.1023/A:1018487401810
  3. Nature Biotechnology v.16 Efficacy of a food plant-based oral cholera toxin B subunit vaccine Arakawa T;Chong DKX;Langridge WHR https://doi.org/10.1038/nbt0398-292
  4. Plant Cell Reports v.20 Synthesis of a cholera toxin B subuint-rotavirus NSP4 fusion protein in potato Arakawa T;Yu J;Langridge WHR https://doi.org/10.1007/s002990000312
  5. The FASEB Journal v.14 Human repiratory syncytial virus vaccine antigen produced in plants Belanger H;Fleysh N;Cox S;Bartman G;Deka D;Trudel M;Koprowski H;Yusibov V https://doi.org/10.1096/fj.00-0144com
  6. Biotechnol Prog v.16 Process options in hepatitis B surface antigen extration from transgenic potato Belgin D;Mason HS;Richter LJ;Hunter JB;Shuler ML https://doi.org/10.1021/bp0000454
  7. Nucl Acid Res v.14 The stucture and transcription start site of a major potato tuber portein gene Bevan M;Barker R;Goldsbrough A;Jarvis M;Kavanagh T;Iturriage G https://doi.org/10.1093/nar/14.11.4625
  8. Transgenic Research v.6 Expression of the human milk protein β-casein in transgenic potato plants Chong DKX;Roberts W;Arakawa T;Illes K;Bagi G;Slattery CW;Langridge WHR https://doi.org/10.1023/A:1018410712288
  9. Phytochemica Bulletin v.19 A rapid DNA isolation procedure for small quantities of fresh tissue Doyle JJ;Doyle JL
  10. Virology v.249 Expression of immunogenic glyco-protein S polypeptides from transmissible gastroenteritis coronavirus in transgenic plants Gomez N;Carrillo C;Salinas J;Parra F;Borca MV;Escribano JM https://doi.org/10.1006/viro.1998.9315
  11. Science v.268 Oral immunization with a recombinant bacterial antigen produced in transgenic plants Haq TA;Mason HS;Clements JD;Arntzen CJ https://doi.org/10.1126/science.7732379
  12. J. Mol Biol v.311 Expression of the native cholera toxin B subunit gene and assembly as functional oligomers in transgenic tobacco chlorplasts Henry D;Seung-Bum L;Tanvi P;Peter OW https://doi.org/10.1006/jmbi.2001.4921
  13. Proc. Natl Acad Sci USA v.98 Oral immunization with hepatitis B surface antigen expressed in transgenic plants Kong Q;Richter L;Yang YF;Arntzen CJ;Mason HS;Thanavala Y
  14. Vaccine v.19 Oral immunisation of native and primed animals with transgenic potato tubers expressing LT-B Lauterslager TGM;Florack DEA;van der Wal TJ;Molthoff JW;Langeveld JPM;Bosch D;Boersma WJA;Hilgers LAT https://doi.org/10.1016/S0264-410X(00)00513-2
  15. Proc Natl Acad Sci USA v.89 Expression of hepatitis B surface antigen in transgenic plants Mason HS;Lam DMX;Arntzen CJ
  16. Proc Natl Acad Sci USA Expression of Norwalk virus capsid protein in transgenic tobacco and potato and its oral immuno-genicity in mice Mason HS;Ball JM;Shi JJ;Jiang X;Estes MK;Arntzen CJ
  17. Vaccine v.16 Edible vaccine protects mice against Escherichia coli heat-labile enterotoxin (LT): Potatoes expressing a synthetic LT-B gene Mason HS;Haq TA;Clements JD;Arntzen CJ https://doi.org/10.1016/S0264-410X(98)80020-0
  18. Biotechnology v.13 Expression of the rabies virus glycoprotein in transgenic tomatoes McGarvey PB;Hammond J;Dienelt MM;Hooper DC;Fu ZF;Dietzschold B;Koprowski H;Michaels FH https://doi.org/10.1038/nbt1295-1484
  19. Current Opinion in Biotechnology v.12 Edible genetically modified microorganisms and plants for improved health Mercenier A;Wiedermann U;Breiteneder H https://doi.org/10.1016/S0958-1669(00)00255-X
  20. Gene v.62 Molecular characterization of the patatin multigene family of potato Mignery GA;Pikaard CS;Park WD https://doi.org/10.1016/0378-1119(88)90577-X
  21. Plant Cell Rep v.20 L-Cysteine increases Agrobacterium-mediated T-DNA delivery into soybean cotyledonary-node cells Olhoft PM;Somers A https://doi.org/10.1007/s002990100379
  22. Infection and Immunity v.69 Towards development of an edible vaccine against bovine pneumonic pasteurellosis using transgenic white clover expressing a mannheimia haemolytica A1 Leukotoxin 50 fusion protein Raymond WHL;Judith S;Doug HD;Patricia ES;Yongqing N;Reggie YCL https://doi.org/10.1128/IAI.69.9.5786-5793.2001
  23. Nature Biotechnology v.18 Production of hepatitis B surface antigen in transgenic plants for oral immuniztion Richter LJ;Thanavala Y;Arntzen CJ;Mason HS https://doi.org/10.1038/81153
  24. Transgenic Research v.9 Oral immunization of mice with transgenic tomato fruit expressing respiratory syncytial virus-F protein induces a systemic immune response Sandhu JS;Krasnyanski SF;Domier LL;Korban SS;Osadjan MD;Buetow DE https://doi.org/10.1023/A:1008979525909
  25. Veterinary Microbiology v.78 Duration of the protection of and E2 subunit marker vaccine against classical swine fever agter a single vaccination de Smit AJ;Bouma A;de Kluijver EP;Terpstra C;Moormann RJM https://doi.org/10.1016/S0378-1135(00)00306-0
  26. Plant Cell Reports v.19 Transgenic tomato plants expressing a Lycopersicon chilense chitinase gene demonstrate improved resistance to Verticillium dahliae race 2 Tabaeizadeh Z;Agharbaoui Z;Harrak H;Poysa V https://doi.org/10.1007/s002990050733
  27. Nat Med v.4 Immunogenicity in gymans of a recombinant bacterial antigen deliversed in a transgenic potato Tacket CO;Mason HS;Losonsky G ;Clements JD;Levine MM;Arntzen CJ https://doi.org/10.1038/nm0598-607
  28. Proc Natl Acad Sci USA v.92 Immunogenicity of transgenic plant-derived gepatitis B surface antigen Thanavala Y;Yang YF;P. Lyons P;Mason HS;Arntzen C
  29. Vaccine v.18 Immunogenicity of porcine transmissible gestroenteritis virus spike protein expressed in plants Tuboly T;Yu W;Bailey A;Degrandis S;Du S;Erickson L;Nagy E https://doi.org/10.1016/S0264-410X(99)00525-3
  30. Vaccine v.19 DNA-mediated protection against classical swine fever virus Xinglong Y;Changchun T;Hongwei L;Rongliang H;Chuangfu C;Zuosheng L;Maolin Z;Zhen; Y https://doi.org/10.1016/S0264-410X(00)00334-0
  31. Genetic engineering of crop plants Tuber-specific gene expression in transgenic potato plants Willmitzer L;Basner A;Frommer W;Hofgen R;Liu XJ;Koster M;Prat s;Rocha-sosa M;Sonnewald U;Vancanneyt G;Lycett D(ed);Grierson D(ed)