Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Isolation and Nucleotide Sequence Analysis of ADP-glucose Pyrophosphorylase gene from Chinese cabbage (Brassica rapa L.)

  • Kim, In-Jung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Park, Jee-Young (Department of Horticulture, College of Agriculture and Life Science, Chungnam National University) ;
  • Lee, Young-Wook (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Chung, Won-Il (Department of Biological Sciences, Korea Advanced Institute of Science and Technology) ;
  • Lim, Yong-Pyo (Department of Horticulture, College of Agriculture and Life Science, Chungnam National University)
  • Published : 2002.06.01
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Abstract

ADP-glucose pyrophosphorylase (AGPase) catalyzes the key regulatory step in starch biosynthesis. Two cDNA clones encoding AGPase subunits were isolated from the leaf cDNA library of Chinese cabbage (Brassica campestris L. spp. pekinensis). One was designated as BCAGPS for the small subunit and the other as BCAGPL for the large subunit. Both cDNAs have uninterrupted open reading frames deriving 57 kDa and 63 kDa polypeptides for BCAGPS and BCAGPL, respectively, which showed significant similarity to those of other dicot plants. Also, However, the deduced amino acid sequence of BCAGPL has a unique feature. That is, it contains two regions (Rl and R2) lacking in all other plant enzymes. This is the first report of BCAGPL containing Rl and R2 among plant large subunits as well as small subunits. From the genomic Southern analysis and BAC library screening, we inferred the genomic status of BCAGPS and BCAGPL gene.

Keywords

References

  1. Plant Mol Biol v.23 Isolation and analysis of a cDNA clone encoding the small subunit of ADP-glucose pyrophosphorylase from wheat Ainsworth C;Tarvis M;Clark J https://doi.org/10.1007/BF00021416
  2. Plant Mol Biol Rep v.9 Nuclear DNA content of some important plant species Arumuganathan K;Earle ED https://doi.org/10.1007/BF02672069
  3. Symp Soc Exp Biol v.31 Conservation of carbohydrate by the nonphotosynthetic cells of higher plants Ap Rees T
  4. Biochem Biophys Res Comms v.257 Heat stability of the potato tuber ADP-glucose pyrophosphorylase: Role of Cys residue 12 in the small subunit Ballicora MA;Fu Y;Frueauf JB;Preiss J https://doi.org/10.1006/bbrc.1999.0469
  5. Plant Physiol v.118 ADP-glucose pyrophosphorylase from potato tubers. Site-directed mutagenesis studies of the regulatory sites Ballicora MA;Fu Y;Nesbitt NM;Preiss J https://doi.org/10.1104/pp.118.1.265
  6. Plant Physiol v.117 Mutagenesis of the glucose-1-phosphate-binding sit of potato tuber ADP-glucose pyrophosphorylase Fu Y;Ballicora MA;Preiss J https://doi.org/10.1104/pp.117.3.989
  7. Plant Physiol v.108 The large subunit of the embryo isoform of ADP-glucose pyrophosphorylase from maize Giroux L;Smith-White B;Gilmore V;Hannah LC;Preiss J https://doi.org/10.1104/pp.108.3.1333
  8. Proc Natl Acad Sci USA v.95 Enhanced stability of maize endosperm ADP-glucose pyrophosphorylase is gained through mutants that alter subunit interactions Greene TW;Hannah LC
  9. Proc Natl Acad Sci USA v.95 Generation of up-regulated alosteric variants of potato ADP-glucose pyrophosphorylase by reversion genetics Greene TW;Kavakli IH;Kahn ML;Okita TW
  10. Nucleic Acids Res v.15 Putative polyadenylation signals in nuclear genes of higher plants:a compilation and analysis Joshi CP https://doi.org/10.1093/nar/15.23.9627
  11. Biosci Biotechnol Biochem v.62 Characterization of cDNAs encoding small and large subunits of ADP-glucose pyrophosphorylases from watermelon (Citrullus vulgaris S.) Kim IJ;Kahng HY;Chung WI https://doi.org/10.1271/bbb.62.550
  12. Korean J Food Sci Technol v.21 Selection scheme of radish varieties to improve storage stabilities of fermented pickled radish cubes with special reference to sugar content Kim KJ;Kim KH;Myung WK;Shim ST;Kim HK
  13. Mol Gen Genet v.246 Molecular cloning and characterization of novel isoforms of potato ADP-glucose pyrophosphorylase La Cognata U;Willmitzer L;Mller-Rber B https://doi.org/10.1007/BF00298960
  14. Plant physiol v.86 Isolation and characterization of a starchless mutant of Arabidopsis thaliana (L) Heynh, Lacking ADP-glucose pyrophos phorylase activity Lin TP;Caspar T;Somerville C;Preiss J https://doi.org/10.1104/pp.86.4.1131
  15. Plant Physiol v.85 kSubunit structure of spinach leaf ADP-glucose pyrophos phorylase Morell MK;Bloom M;Knowles V;Preiss J https://doi.org/10.1104/pp.85.1.182
  16. Gene v.206 Molecular cloning and organspecific expression of three isoforms of tomato ADP-glucose pyrophosphorylase gene Park SW;Chung WI https://doi.org/10.1016/S0378-1119(97)00588-X
  17. J Plant Res v.111 Molecular cloning and characterization of small and large subunits of ADP-glucose pyrophosphorylase from oriental melon Park SW;Kahng HY;Kim IJ;Park JO;Chung WI https://doi.org/10.1007/BF02507150
  18. Foods and Biotechnology v.4 Effect of starch sources on yulmoo kimchi fermentation Park WP
  19. Surveys of Plant Molecular and Cell Biology v.17 Biology and molecular biology of starch synthesis and its regulation Preiss J;Miflin BJ(ed.)
  20. Molecular Cloning: A Laboratory Manual(2nd Ed.) Sambrook J;Fritsch EF;Maniatis T
  21. Proc Nat'l Acad Sci USA v.74 DNA sequencing with chain termination inhibitors Sanger F;Nicklen S;Coulson R
  22. J Mol Evol v.34 Comparison of proteins of ADP-glucose pyrophosphorylase from diverse sources Smith-White BJ;Preiss J https://doi.org/10.1007/BF00162999
  23. Nucleic Acid Res v.22 Clustal W; Improving the sensitivity of progressive multiple sequence alignment through sequence weighting, positions-specific gap penalties and weight matrix choice Thmopson JD;Higgins AG;Gibson TJ https://doi.org/10.1093/nar/22.22.4673
  24. Nucleic Acid Res v.17 A small-scale procedure for the rapid isolation of plant RNAs Verwoerd TC;Dekker BM;Hoekema A https://doi.org/10.1093/nar/17.6.2362
  25. Plant Physiol v.100 ADP-glucose pyrophosphorylase large subunit cDNA from barley endosperm Villand P;Olsen OA;Kilian LA;Kleczkowski L https://doi.org/10.1104/pp.100.3.1617
  26. Plant Mol Biol v.23 Molecular characterization of multiple cDNA clones for ADP-glucose pyrophosphorylase from Arabidopsis thaliana Villand P;Olsen OA;Kleczkowski LA https://doi.org/10.1007/BF00042361