Journal of Plant Biotechnology

한국식물생명공학회 (The Korean Society of Plant Biotechnology)
권호 표지

Identification of Fruit-specific cDNAs in a Ripened Inodorus Melon Using Differential Screening and the Characterization of on Abscisic Acid Responsive Gene Homologue

  • Hong, Se-Ho (Department of Biological Sciences, Korean Advanced Institute of Sciences and Technology) ;
  • Kim, In-Jung (Department of Biological Sciences, Korean Advanced Institute of Sciences and Technology) ;
  • Chung, Won-Il (Department of Biological Sciences, Korean Advanced Institute of Sciences and Technology)
  • 발행 : 2002.03.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Eight cDNAs corresponding to fruit-specific genes were isolated from ripened melon through differential screening. Sequence comparison indicated that six of these cDNAs encoded proteins were previously characterized into aminocyclopropane-1-carboxylate (ACC) oxidase, abscisic acid, stress and ripening inducible (ASR) gene, RINC-H2 zinc finger protein, pyruvate decarboxylase, or polyubiquitin. RFS2 and RFS5 were the same clone encoding polyubiquitin. The other cDNAs showed no significant homology with known protein sequences. The ASR homologue (Asr1) gene was further characterized on the cDNA and genomic structure. The deduced amino acid sequence had similar characteristics to other plant ASR. The Asr1 genomic DNA consisted of 2 exons and 1 intron, which is similar to the structure of other plants ASR genes. The promoter region of the Asr1 gene contained several putative functional cis-elements such as an abscisic acid responsive element (ABRE), an ethylene responsive element (ERE), a C-box or DPBf-1 and 2, Myb binding sites, a low temperature responsive element (LTRE) and a metal responsive element (MRE). The findings imply that these elements may play important roles in the response to plant hormones and environmental stresses in the process of fruit development. The results of this study suggest that the expressions of fruit specific and ripening-related cDNAs are closely associated with the stress response.

키워드

참고문헌

  1. Plant Molecular Biology v.33 Characterization of two cDNA clones for mRNAs As expressed during ripening of melon (Cucumis melo L.) fruits Aggelis A;John I;Karvouni Z;Grierson D https://doi.org/10.1023/A:1005701730598
  2. Plant Cell v.12 Identification of the SAAT gene involoved in strawberry flavor biogenesis by use of DNA microarrays Aharoni A;Keizer LCP;Bouwmeester HJ;Sun Z;Alvarez-Huerta M;Verhoeven HA;Blaas J;van Houwelingen AMML;De Vos RCH;van der Voet H;Jansen RC;Guis M;Mol J;Davis RW;Schena M;van Tunen AJ;O'Connell AP https://doi.org/10.1105/tpc.12.5.647
  3. Plant Physiol v.107 Dissection of oxidative stress tolerance using transgenic plants Allen RD https://doi.org/10.1104/pp.107.4.1049
  4. Plant Physiol v.106 Genomic nucleotide sequence of tomato Asr2, a second member of the stress/ripening-induced Asrl gene family Amitai-Zeigerson H;Scolnik PA;Bar-Zvi D https://doi.org/10.1104/pp.106.4.1699
  5. Plant Sci v.110 Tomato Asr1 mRNA and protein are transiently ex pressed following salt stress, osmotic stress and treatment with abscisic acid Amitai-Zeigerson H;Scolnik PA;Bar-Zvi D https://doi.org/10.1016/0168-9452(95)94515-K
  6. Nature Biotechnol v.14 Expression of ACC oxidase antisense gene inhibits ripening of centaloupe melon fruits Ayub R;Guis M;Ben Amor M;Gillot L;Roustan JP;Latche A;Bouzayen M;Pech JC https://doi.org/10.1038/nbt0796-862
  7. Eur J Biochem v.212 Isolation of a ripening and woundinduced cDNA from Cucumis melo L. encoding a protein with homology to the ethylene-forming enzyme Balaque C;Watson CF;Turner AJ;Rouge P;Picton S;Pech JC;Grierson D https://doi.org/10.1111/j.1432-1033.1993.tb17628.x
  8. Plant Mol Bio v.37 Regulation of abscisic acid-induced transcription Busk PK;Pages M https://doi.org/10.1023/A:1006058700720
  9. Plant Physiol v.108 Pumelo fruit transcript homologous to ripening-induced genes Canel C;Bailey-Serres JN;Roose ML https://doi.org/10.1104/pp.108.3.1323
  10. Plant Physiol v.121 Lily (Lilium longiflorum) transcript (Accession No. AF077629) induced by desiccation during pollen development (PGR99-159) Chang FC;Wang CS https://doi.org/10.1104/pp.121.3.1053
  11. Physiol Plant v.97 Gene expression under water deficit in loblolly pine (Pinus taeda L.): Isolation and characterization of cDNA clones Chang S;Puryear JD;Dias DMAL;Funkhouser EA;Newton RJ;Cairney J https://doi.org/10.1111/j.1399-3054.1996.tb00490.x
  12. Plant Mol Biol Rep v.1 Plant DNA minipreparation: Version II Dellaporta SL;Wood J;Hicks JBA
  13. Plant Cell v.12 cDNA-AFLP reveals a striking overlap in race-specific resistance and wound response gene expression profiles Durrant WE;Rowland O;Piedras P;Hammond-Kosack KE;Jones JD https://doi.org/10.1105/tpc.12.6.963
  14. Plant Mol Biol v.20 Expression of stress-responsive ubiquitin genes in potato tubers Garbarino JE;Rockhold DR;Belknap WP https://doi.org/10.1007/BF00014491
  15. Gene v.148 Structure and promoter activity of a stress and developmentally regulated polyubiquitin-encoding gene of Nicotiana tabacum Genschik P;Marbach J;Uze M;Feuerman M;P;esse B;Fleck J https://doi.org/10.1016/0378-1119(94)90689-0
  16. Acta Hort v.447 Asr1, a tomato water-stress regulated gene: Genomic organization, developmental regulation and DNA-binding activity Gilad A;Amitai-Zeigerson H;Scolnik PA;Bar-Zvi D
  17. Plant Physiol v.113 Genomic nucleotide sequence of the tomato stress/ripening induced Asr1 gene (Accession No. U86130) (PGR97-042) Gilad A;Amitai-Zeigerson H;Scolnik PA;Bar-Zvi D
  18. Ann Rev Plant Physiol Plant Mol Biol v.52 Molecular biology of fruit maturation and ripening Giovannoni J https://doi.org/10.1146/annurev.arplant.52.1.725
  19. J Biol Chem v.261 Differential and common recognition of the catalytic sites of the cGMP-dependent and cAMP-dependent protein kinases by inhibitory peptides derived from the heat-stable inhibitor protein Glass DB;Cheng HC;Kemp BE;Walsh DA
  20. Plant Physiol v.122 Characterization of ripening-regulated cDNAs and their expression in ethylene-suppressed Charentais melon fruit Hadfield KA;Dang T;Guis M;Pech J-C;Bouzayen M;Bennett AB https://doi.org/10.1104/pp.122.3.977
  21. Plant Physiol v.117 Polygalacturonase gene expression in ripen melon fruit supports a role for polygalacturonase in ripening-associated pectin disassembly Hadfield KA;Rose JKC;Yaver DS;Berka RM;Bennett AB https://doi.org/10.1104/pp.117.2.363
  22. Plant Sci v.159 Cloning of an auxin-responsive 1-aminocyclopropane-1-carboxylate synthase gene (CMe-ACS2) from melon and the expression of ACS genes in etiolated melon seedlings and melon fruits Ishiki Y;Oda A;Yaegashi Y;Orihara Y;Arai T;Hirabayashi T;Nakagawa H;Sato T https://doi.org/10.1016/S0168-9452(00)00298-3
  23. J Exp Bot v.51 Isolation of cDNA clones corresponding to genes expressed during fruit ripening in Japanese pear (Pyrus pyrifolia Nakai): involvement of the ethylene signal transduction pathway inn their expression Itai A;Tanabe K;Tamura F;Tanaka T https://doi.org/10.1093/jexbot/51.347.1163
  24. Plant Mol Biol v.27 Isolation and characterization of a melon cDNA clone encoding phytoene synthase Karvouni Z;Hohn I;Taylor JE;Watson CF;Turner AJ;Grierson D https://doi.org/10.1007/BF00020888
  25. Plant Sci v.133 Molecular characterization of a cytosolic ascorbate peroxidase in strawberry fruit Kim IJ;Chung WI https://doi.org/10.1016/S0168-9452(98)00029-6
  26. Mol Biol Cell v.11 The APC11 RING-H2 finger mediates E2-dependent ubiquitination Leverson JD;Joazeiro CA;Page AM;Huang HK;Hieter P;Hunter T https://doi.org/10.1091/mbc.11.7.2315
  27. J. Agri Food Chem v.47 Role of ethylene and abscisic acid in physicochemical modifications during melon ripening Martinez-Madrid M;Martnez G;Pretel MT;Serrano M;Romojaro F https://doi.org/10.1021/jf990035m
  28. Plant Physiol v.115 Molecular cloning and nucleotide sequence of an abscisic acid-sress-ripening induced (ASR)-like protein from apricot fruit (acc. No. U93164). Gene expression during fruit ripening. (PGR97-166) Mbeguie-A-Mbeguie D;Gomez RM;Fils-Lucaon B
  29. Proc Natl Acad Sci USA v.90 Identification of an ethylene-responsive region in the promoter of a fruit ripening gene Montgomery J;Goldman S;Deikman J;Margossian L;Fischer RL
  30. Nature Biotechnol v.19 Over expression of petunia chalcone isomerase in tomato results in fruit containing increased levels of flavonols Muir SR;Collins GJ;Robinson S;Hughes S;Bovy A;Ric De Vos CH;van Tunen AJ;Verhoeyen ME https://doi.org/10.1038/88150
  31. Plant Mol Biol v.35 Expression analysis of a gene family in loblolly pine (Pinus taeda L.) induced by water deficit stress Padmanabhan V;Dias DMAL;Newton RJ https://doi.org/10.1023/A:1005897921567
  32. Nature Biotechnol v.18 Elevation of the provitamin A content of transgenic tomato plants Romer S;Fraser PD;Kiano JW;Shipton CA;Misawa N;Schuch W;Bramley PM https://doi.org/10.1038/76523
  33. Plant Physiol v.117 Temporal Sequence of cell wall disassembly in rapidly ripening melon fruit Rose JKC;Hadfield KA;Labavitch JM;Bennett AB https://doi.org/10.1104/pp.117.2.345
  34. Mol Gen Genet v.252 Asr genes belong to a gene family comprising at least three closely linked loci on chromosome 4 in tomato Rossi M;Lijavetzky D;Bernacchi D;Hopp HE;Iusem N
  35. Plant Mol Biol v.40 Early elicitor induction in members of a novel multigene family coding for highly related RING-H2 proteins in Arabidopsis thaliana Salinas-Mondragon RE;Garciduenas-Pina C;Guzman P https://doi.org/10.1023/A:1006267201855
  36. Molecular cloning, A Laboratory Manual (2nd ed). Sambrook J;Russell DW
  37. Proc Natl Acad Sci USA v.74 DNA sequencing with chain terminating inhibitors Sanger F;Nicklen S;Coulson R
  38. Plant Physiol v.119 Stage-and tissue-specific expression of ethylene receptor homolog genes during fruit development in muskmelon Sato-Nara K;Yuhashi KI;Higashi K;Hosaya, K.;Kubota M;Ezura H https://doi.org/10.1104/pp.119.1.321
  39. Plant Physiol v.113 Expression patterns and promoter activity of the cold-regulated gene ci21A of potato Schneider A;Salamini F;Gebhardt C https://doi.org/10.1104/pp.113.2.335
  40. Biochemistry of fruit ripening Melons Seymour GB;McGlasson WB;Seymour GB(ed.);Taylor JE(ed.);Tucker GA(ed.)
  41. J Agri Food Chem v.49 Acetyl-coA: Alcohol acetyltransferase activity and aroma formation in ripening melon fruits Shalit M;Katzir N;Tadmor Y;Larkov O;Burger Y;Shalekhet F;Lastochkin E;Ravid U;Amar O;Edelstein M;Karchi Z;Lewinsohn E https://doi.org/10.1021/jf001075p
  42. Plant Mol Biol v.27 Isolation and characterization of a water stress-inducible cDNA clone from Solanum chacoense Silhavy D;Hutvagner G;Barta E;Banfalvi Z https://doi.org/10.1007/BF00019324
  43. Ann Rev Plant Physiol Plant Mol Biol v.46 Light-regulated transcription Terzaghi WB;Cashmore AR https://doi.org/10.1146/annurev.pp.46.060195.002305
  44. Ann Rev Biochem v.57 The biology and enzymology of eukaryotic protein acylation Towler DA;Gordon JI;Adams SP;Glaser L https://doi.org/10.1146/annurev.bi.57.070188.000441
  45. Plant Sci v.140 Characterization and expression pattern of an abscisic acid and osmotic stress responsive gene from rice Vaidyanathan R;Kuruvilla S;Thomas G
  46. Plant J v.5 The basic domain in the bZIP regulatory protein Opaque2 serves two independent functions: DNA binding and nuclear localization Varagona MJ;Raikhel NV https://doi.org/10.1046/j.1365-313X.1994.05020207.x
  47. Nucl Acids Res v.17 A small-scale procedure for the repid isolation of plant RNAs Verwoerd TC;Dekker BM;Hoekema A https://doi.org/10.1093/nar/17.6.2362
  48. Plant Cell Physiol v.39 Characterization of a desiccation-related protein in lily pollen during development and stress Wang CS;Liau YE;Huang JC;Wu TD;Su CC;Lin CH https://doi.org/10.1093/oxfordjournals.pcp.a029335
  49. Can J Forest Research v.27 Sequence analysis and ozone-induced accumulation of polyubiquitin mRNA in Pinus sylvestris Wegener A;Gimbel W;Werner T;Hani J;Ernst D;Sandermann H https://doi.org/10.1139/cjfr-27-6-945