Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Plant Regeneration of Major Cultivars of Sweetpotato (Ipomoea batatas) in Korea via Somatic Embryogenesis

체세포배발생을 통한 국내 주요 고구마 품종의 식물체 재분화

  • 권은정 (한국생명공학연구원 식물세포공학연구실, 목원대학교 생명과학과) ;
  • 권석윤 (환경생명공학연구실) ;
  • 김문자 (목원대학교 생명과학과) ;
  • 안영섭 (호남농업시험장 목포시험장) ;
  • 이준설 (호남농업시험장 목포시험장) ;
  • 정병춘 (호남농업시험장 목포시험장) ;
  • 곽상수 (환경생명공학연구실) ;
  • 이행순 (한국생명공학연구원 식물세포공학연구실)
  • Published : 2002.09.01
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Abstract

An efficient plant regeneration system of major cultivars of sweetpotato (Ipomoea batatas (L.) Lam.) in Korea via somatic embryogenesis was established. Embryogenic calli were formed from shoot apical meristems of sweetpotato cultivars when cultured on LS medium supplemented with 1 mg/L auxin (2,4-D, picloram, dicamba). Among three kinds of auxin, 1 mg/L 2,4-D showed the highest embryogenic calli induction rate. After 4 weeks of cultures on LS medium supplemented with 1 mg/L 2,4-D, embryogenic calli induction rates of Sinhwangmi, Zami, Yulmi, and White Star were 86%, 78%, 76%, and 80%, respectively. Upon transfer onto LS basal medium, most of somatic embryos developed into plantlets. Regenerated plantlets were transplanted to potting soil and grown to mature plants in a greenhouse.

4종류 고구마 품종을 대상으로 정단분열조직 배양에 의한 배발생 캘러스 유도, 이들로부터 고빈도의 체세포배발생과 식물체 재분화 체계를 확립하였다. 자미. 율미, 신황미, 및 화이트 스타의 생장점을 LS 기본배지에 1 mg/L auxin (2,4-D, picloram, dicamba)가 첨가된 배지에서 배양하였다. 그 결과 2,4-D 첨가 배지에서 가장 높은 빈도로 배발생 캘러스가 유도되었으며, 배양 4주째에 유도된 배발생 캘러스의 빈도는 신황미, 자미, 율미. 및 화이트 스타에서 86%, 78%, 76%, 및 80%를 각각 나타내었다. 2,4-D 첨가 배지에서 얻어진 배발생 캘러스를 2.4-D를 제거한 배지로 옮겨 주어 체세포배를 유도하였으며, 이 체세포배의 90% 이상이 완전한 식물체로 발달하였다. 이러한 결과는 정단분열조직 배양으로부터의 체세포배 발생 방법이 광범위한 고구마 품종에 적용될 수 있을 뿐만 아니라 분자육종을 통한 신기능성 고구마 품종개발에 효율적으로 이용될 수 있을 것으로 기대된다.

Keywords

References

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