Examination of Genetic Relationships of Silkworm Stocks in Korea by Additive Isozyme Analysis

  • Sohn, Bong-Hee (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Kim, Hyun-Su (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Kang, Pil-Don (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Lee, Sang-Uk (Department of Sericulture and Entomology, National Institute of Agricultural Science and Technology) ;
  • Seong, Su-Il (Department of Life Science, College of National Sciences, The University of Suwon)
  • Published : 2002.12.01

Abstract

Previously, genetic relationships among 321 silkworm races preserved in the Department of Sericulture and Entomology, NIAST, RDA were studied using six isozymes comprising 20 loci. As a part of additional studies, three additional isozymes with 7 loci [phos-phoglucomutase (PGM), glucose phosphate isomerase (GPI), malete dehydrogenase (MDH)] from hemolymphs, midgets, and eggs were employed to reevaluate the previous dendrogram (UPGMA method). Although the current study supported the recognition of the previously identified major groups, some minor changes were apparent among the selected 47 forms. The current study showed that the polymorphic loci of GPI from eggs and MDH from hemolymph appear to be responsible characters for generating a new major group in the genetic relationship. Interpreting the data with current additive isozymes may represent more robust genetic relatioships among 321 silkworm races preserved in Korea, until further evidence is available.

Keywords

References

  1. Eguchi, M., Y. Takahama, M. Ikeda, and S. Horii (1988) A novel variant of acid-phosphatase isozyme from hemolymph of the silkworm, Bomyx mori. Jpn. J. Genet. 63, 149-157 https://doi.org/10.1266/jjg.63.149
  2. Kanekatsu, R., M. Hori, and K. Igeta (1992) Presence of three sucrases with different molecular weights in the larval midgut of the silkworm, Bombyx mori. J. Seric. Sci. Jap. 61, 6-14
  3. Kim, I., J. S. Bae, H. D. Sohn, P. D. Kang, K. S. Ryu, B. H. Sohn, W. B. Jeong and B. R. Jin (2000) Genetic homogeneity in the domestic silkworm, Bombyx mori, and phylogenetic relationship between B. mori and the wild silkworm, B. mandarina using mitochondrial COI gene sequences, Int. J. Indust. Entomol. 1, 19-17
  4. Norusis, M. J. (1986) SPSS/PC + for the IBM PC/XT/AT, SPSS INC., Chicago
  5. Seong, S. I. (1997) Genetic Relationships of Silkworm Stocks in Korea. Korean J. Seric. Sci. 39, 119-133
  6. Takeda, S., M. Azuma, M. Itoh, and M. Eguchi (1993) The strain difference of polymorpic nature of membrane-bound alkaline phosphatase in the midgut epithelium of the silkworm, Bombyx mori. Comp. Biochem. Physiol. 104, 81-89 https://doi.org/10.1016/0305-0491(93)90341-2
  7. Yoon, H. J., J. S. Bae, I. Kim, B. R. Jin and Y. I. Mah (2001) A phyolgenetic study in some long-horned beetles (Coleoptera: Cerambycidae) using mitochondrial COI gene and 16S rRNA sequences, Int. J. Indust. Entomol. 2, 37-53