International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Effect of Systemic Fungicide on Total Hemocyte Count and Hemolymph Biochemical Changes in Silkworm, Bombyx mori L., infected with Beau-veria bassiana

  • Mallikarjuna, M. (Central Sericultural Research and Training Institute) ;
  • Nataraju, B. (Central Sericultural Research and Training Institute) ;
  • Thiagarajan, V. (Central Sericultural Research and Training Institute)
  • Published : 2002.12.01
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Abstract

Silkworm diseases are major constraint in silk cocoon production. Among silkworm diseases, white muscar-dine is highly contagious and most common in winter and rainy seasons. It is suggested that hemocytes involve in defense against invasion of Beauveria bassi-ana and systemic fungicide/chemicals prevent the proliferation of fungi in the hemolymph or preventing the growth of the fungi in the body cavity through enhancing the hemocyte mediated defense response. In the present study the influence of systemic fungicide on hematological changes in silkworms infected with Beauveria bassiana was reported. It is observed that the total hemocyte counts increased in the hemolymph up to 5 days post inoculation in systemic fungicide treated batches while in the inoculated control the increase was up to 3 days indicating the positive hemocyte mediated response in silkworm treated with systemic fungicide. After 2 days in the inoculated control as the multiplication and growth of mycelia increased, defense capacity of the silkworm was decreased. The biochemical changes were also observed in the hemolymph of silkworm infected with B. bassiana. In silkworm infected with Beauveria bassi-ana, the total protein content increased whereas total carbohydrate and total lipids decreased as the infection progresses. In the case of systemic fungicide treated batches the increase in total protein content was comparatively higher and decrease in total carbohydrate and lipids were comparatively lower than the inoculated control.

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References

  1. Bidochka, M. J. and Hajek, A. E. (1998) A Nonpermissiove Entomophthoralean Fungal Infection Increases Activation of Insect Prophenoloxidase. J. Invertebr. Pathol. 72, 231-238 https://doi.org/10.1006/jipa.1998.4782
  2. Butt, T. M., S. P. Wraight, Galaini, S. Wraight, R. A. Humber, D. W. Roberts and R. S. Soper (1988) Humoral encapsulation of the fungus Erynia radicans (Entomophth orales) by the potato leaf hooper, Empoasca fabae (Homoptera-Ci cadellidae). J. Invertebr. Pathol. 52, 49-56 https://doi.org/10.1016/0022-2011(88)90101-2
  3. Butt, T. M. and R. A. Humber (1989) Response of gypsy moth hemocytes to natural fungal protoplasts of three Entomophager species (Zygomycotina Entomophthorates). J. Invertebr. Pathol. 53, 121-123 https://doi.org/10.1016/0022-2011(89)90084-0
  4. Chain, B. M. and R. S. Anderson (1982) Selective depletion of the plasmatocytes in Galleria mellonella following injection of bacteria. J. Insect. Physiol. 28, 377-384 https://doi.org/10.1016/0022-1910(82)90051-8
  5. Chino, H. and L. I. Gilbert (1965) Lipid release and transport in insects. Biochem. Bio. Phys. Acta 98, 94-110 https://doi.org/10.1016/0005-2760(65)90014-7
  6. Dubios, M., K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith (1956) Colorimetric method of determination of sugars and related substances, Anal. Chem. 28, 350-356 https://doi.org/10.1021/ac60111a017
  7. Dunn, P. E. and D. R. Drake (1983) Fate of bacteria injected into native and immunized larvae of the tobacco hornworm, Manduca sexta. J. Invertebr. Pathol. 41, 77-85 https://doi.org/10.1016/0022-2011(83)90238-0
  8. Folch, I., I. Ascoli, M. Less, J. A. Meath and F. N. Le Baron (1951) Preparation of lipid extracts from brain tissue. J. Biol. Chem. 191, 833-841
  9. Gardner, W. A., R. M. Sutton and R. Noblet (1979) Effects of infection by Beauveria bassiana on hemolymph proteins of noctuid larvae. Ann. Entomol. Soc. Am. 72, 224-228 https://doi.org/10.1093/aesa/72.2.224
  10. Horohov, D. W. and P. E. Dunn (1983) Phagocytosis and nodule formation by hemocytes of Manduca sexta larvae following injection of Pseudomonas aeruginosa. J. Invertebr. Pathol. 41, 203-213 https://doi.org/10.1016/0022-2011(83)90220-3
  11. Hou, R. F. and J. Chang (1985) Cellular Defense Response to Beauveria bassiana in the Silkworm, Bombyx mori. Appl. Ent. Zool. 20, 118-125 https://doi.org/10.1303/aez.20.118
  12. Jones, J. C. (1962) Current concepts concerning insect hemocytes. Amer. Zool. 2, 209-246 https://doi.org/10.1093/icb/2.2.209
  13. Kanost, M. R., J. K. Kawooya, J. H. Law, R. 0. Ryan, M. C. Van Heusden and R. Ziegler (1990) Insect hemolymph proteins, Adv. Insect Physiol. 22, 299-396 https://doi.org/10.1016/S0065-2806(08)60008-9
  14. Kawakami, K. (1965) Phagocytosis in Muscardine-Diseased Larvae of the Silkworm, Bombyx mori (Linnaeus). J. Invertebr. Pathol. 7, 203-208 https://doi.org/10.1016/0022-2011(65)90039-X
  15. Kusunoki, J. and H. Watanabe (1982) Changes in the amino acid composition of the hemolymph from the fifth instar larvae Bombyx mori infected with Beauveria bassiana. J. Seric. Sci. Jpn. 51, 517-522
  16. Lowry, O. H., N. J. Rosebrough, A. L. Farr and R. J. Randall (1951) Protein measurement with the folin-phenol reagent. J. Biol. Chem. 193, 265-275
  17. Pombo, V., L. M. Vellose, B. M. Ribeiro and Bao, S. N. (1998) Structural and ultrastructural changes during the infection of UFL-AG-286 Cells with the Baculovirus AgMNPV. J. Invertebr. Pathol. 72, 239-245 https://doi.org/10.1006/jipa.1998.4788
  18. Salt, G. (1970) The cellular defense reactions of insects. Cambridge Monograph in Experimental Biology, No. 16, Cambridge Univ. Press. London
  19. Tanada, Y. and H. K. Kaya (1993) Insect Pathology, Academic Press, San Diego, California, USA. p. 529
  20. Tauber, 0. E. and J. F. Yeager (1934) On the total hemolymph (blood) cell count of the field cricket Gryllus assimillis pennsylvanicus. J. Sci. 9, 1324
  21. Tauber, 0. E. and J. F. Yeager (1935) On the total blood counts of insects. I. Orthoptera, Odonata, Hemiptera and Homoptera. Ann. Entomol. Soc. Am. 28, 229-240 https://doi.org/10.1093/aesa/28.2.229
  22. Vey, A. and P. Gotz (1986) Anti-fungal cellular and defense mechanism in insects, In 'Hemocytic and Humoral Immunity in Arthropods' (A. P. Gupta, ed.), pp. 90-115, Wiley, New York
  23. Virendrakumar, B. Nataraju and R. K. Datta (1997) Studies on the efficacy of certain systemic fungicides on the control of white muscardine in silkworm, Bombyx mori L. Paper presented in Current Technology Seminar on Silkworm Disease Management, Silkworm Rearing Technology and Mulberry Pathology. C. S. R. & T. I., Berhampore, India
  24. Wago, H. (1995) Host defense reactions of insects. Appl. Ent. Zool. 39, 1-13 https://doi.org/10.1303/jjaez.39.1