A Study on the Antigen Characteristics of Rhodotorula rubra

Rhodotorula rubra의 항원특성에 관한 연구

  • Kwon, Hyuk-Ku (Graduate School of Venture, Hoseo University) ;
  • Lee, Jang-Hoon (College of Environment & Safety Engineering, Hoseo University) ;
  • Ryeon, Kon (Department of Microbiology Dankook University)
  • 권혁구 (호서대학교 벤처 전문대학원) ;
  • 이장훈 (호서대학교 환경안전 공학부) ;
  • 염곤 (단국대학교 미생물학과)
  • Published : 2002.12.01

Abstract

Antigenicity of Rhodotrula rubra isolated from pulmonary tissue of pulmonary tuberculosis patients was studied by means of agglutination reaction with R. rubra whole cell antiserum. And the serological reactivity of crude polyfac charide from R. frubra, Candida albicans, Candida tropicalis, Candida, glabrata, and Saccharomyces cerevisiae ATCC 26603 with antiserum to R. rubra whole cell was studied by means of immunodiffusion test. R. rubra showed stationary phase after 48h when it was cultured in GYEP broth. While agglutinogen titer was 1:64 at lag phase, agglutinogen titer was 1 :256 after 20h. After growth of R. rubra on different 11 media, nutritional environment showed similar agglu-tination reartivity. The agglutinogen titer of C. albicans, C. tropicalis, C. giabrata, which were isolated from patient's expectoration, to R. rubra antiserum by means of agglutination reaction were 1:16, respectively. But, Sacch. cervisiae ATCC26603 was negative. Those results were lower than that of R. rubra agglutinogen titer 1:256. As a result of immu-nodiffusion test with crude polysaccharide extracted from cell wall of R. rubra, C. albicans, C. tropicalis, C. glabrata, Sacch. cervisiae ATCC26603, precipitin line was found only with R. rubra, of which antibody titer was 8.

Keywords

References

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