Biomedical Science Letters (대한의생명과학회지)
- Volume 8 Issue 4
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- Pages.229-234
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- 2002
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- 1738-3226(pISSN)
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- 2288-7415(eISSN)
Effect of Chlorella Growth Factor on the Proliferation of Human Skin Keratinocyte
- Yong-Ho Kim (Department of Biomedical Laboratory Science, Inje University) ;
- Yoo-Kyeong Hwang (Department of Biomedical Laboratory Science, Inje University) ;
- Yu-Yon Kim (Department of Biomedical Laboratory Science, Inje University) ;
- Su-Mi Ko (Department of Biomedical Laboratory Science, Inje University) ;
- Jung-Min Hwang (Department of Biomedical Laboratory Science, Inje University) ;
- Yong-Woo Lee (Department of Biomedical Laboratory Science, Inje University)
- Published : 2002.12.01
Abstract
Chlorella is rich in chlorella growth factor (CGF). A review of the literature has described that CGF improves the capability of a Th1-based immunity, anticancer, antioxidant antibacterial activity, growth promotion, wound healing and so on, but has not studied the effect for the metabolism and the proliferation of human skin keratinocyte. The aim of this study was to examine the effect of metabolism and the proliferation of human skin keratinocyte in vitro. CGF was extracted with an autoclaving method which is a modified hot-water extraction method from dried chlorella and conformed by means of absorbance 0.22 at 260 nm. We have measured the extracellular acidification rate (ECAR) of the CGF by Cytosensor
Keywords
- Chlorella growth factor (CGF);
- Human epidermal keratinocyte;
- HaCaT cell;
- Epidermal growth factor receptor (EGFR);
- Proliferation;
- Cytosensor$^{\circledR}$ Microphysiometer