The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
권호 표지

Screening of 3-Hydroxy-3-Methylglutaryl-Coenzyme A Reductase Inhibitors In Vitro and Its Application to Pullets

HMG-CoA Reductase의 저해제 탐색과 가금의 콜레스테를 저하 효과

  • Moon, Young-Ja (Department of Food and Nutrition, Woosong Information College) ;
  • Yeom, Keum-Hee (Department of Food Science and Technology, Chung Nam National University) ;
  • Sung, Chang-Keun (Department of Food Science and Technology, Chung Nam National University)
  • Published : 2002.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

The primary objective of these studies was to screen the materials showing inhibitions of HMG-CoA reductase in vitro. The secondary objective was to determine the effect of garlic, lovastatin and copper on cholesterol concentrations in plasma, liver and breast tissues in pullets. The degree of inhibition of the selective samples on HMG-CoA reductase activity was determined in vitro. The inhibition ratios of water soluble garlic extracts, lovastatin (methanol extracts) and copper to HMG-CoA reductase activity were 51.3%, 87.5%, and 82.0%, respectively. Control diet (basal diet) and experimental diets, garlic powder (3% in diet), lovastatin (300mg/Kg of diet) and copper (200mg/Kg of diet) were fed to pullets in order to investigate the changes of cholesterol concentration in plasma and tissues. Total cholesterol, HDL- and LDL-cholesterol in blood plasma were significantly reduced in pullets fed diet containing 3% garlic powder. However, copper significantly increased total cholesterol compared to control and lovastatin did not affect plasma cholesterol concentration. Total cholesterol and triglyceride of liver and breast tissues in pullets were not affected by adding the cholesterol-lowering materials to diets. The data suggests that it is not easy for HMG-CoA reductase inhibitors to reduce cholesterol levels in body due to complication of cholesterol metabolism. However, garlic administration can lower the levels of plasma cholesterol in pullets.

체내 cholesterol 수치를 낮추기 위하여 HMG-CoA reductase 활성을 저해하는 물질들을 닭에게 급여하여 in vitro상에서 검토한 결과, HMG-CoA reductase 활성에 대한 마늘, lovastatin과 copper의 순으로 저해능은 51.3%, 87.5%, 82.0%이었다. 또한 혈장, 가슴조직, 간장의 cholesterol 함량에 대한 마늘(3% in diet), lovastatin(300mg/Kg of diet), copper(200mg/Kg of diet)의 효과를 검토하였다. 혈장 중의 cholesterol 함량과 HDL, LDL cholesterol 함량은 3% 마늘의 투여로 감소되어졌으나 copper의 투여는 오히려 증가하였고, lovastatin 투여에 의한 차이는 나타나지 않았다. 그리고 간장과 가슴조직의 cholesterol과 triglyceride는 사료에 cholestrol의 수치를 저하시키는 물질을 첨가하였으나 별다른 차이가 없었다.

Keywords

References

  1. Hargis, P. S. : Modifying egg yolk cholesterol in the domestic fowl - a review, World's Poultry Sci. J., 44, 17 (1988) https://doi.org/10.1079/WPS19880002
  2. Ziegler, D. M. : Role of reversible oxidation-reduction of enyme thiols-disculfides in metabolic regulation. Ann. Rev. Biochem., 54, 305 (1985) https://doi.org/10.1146/annurev.bi.54.070185.001513
  3. Bordia, A. : Effect of garlic on blood lipid in patients with coronary heart disease, Am. J. Clin. Nutr., 34, 2100 (1981)
  4. Sklan, D., Berner, Y. N. and Rabinowitch, H. D. : The effect of dietary onion and garlic on hepatic lipid concentrations and activity of antioxidative enzymes in chicks. J. Nutr. Biochem., 3, 322 (1992) https://doi.org/10.1016/0955-2863(92)90022-B
  5. Klevay, L. M. : Hypercholesterolemia in rats produced by and increase in the ratio of zinc to copper ingested. Am. J. Clin Nutr., 26, 1060 (1973)
  6. Henwood, J. M., Heel, R. C. : Lovastatin: A preliminary reveiw of its pharacodynamic properties and therapeutic use in hypercholesterolemia. Drugs, 36, 429 (1988) https://doi.org/10.2165/00003495-198836040-00003
  7. Lee, Y. H., Shin, Y. M., Lee, J. E., Chio, Y. S., Lee, S. Y. : In vitro screening of 3-hydroxy-3-methylglutaryl coenzyme A reductase inhibitor from plants extracts. Kor. J. Biotechnol. Bioeng., 6, 55 (1991)
  8. Lowry, O. H., Rosebrough, N. J., Farr, A. L. and Randall, R. L. : Protein measurement with the folin phenol reagent. J. Biol. Chem., 193, 265 (1951)
  9. Hulcher, F. H., and Olesson, W. H. : Simplified spectrophotometric assy for microsmal-3-hydroxy-3-methylglutaryl Co A reductase by measurement of coenzyme. Am. J. Lipid Res., 14, 625 (1987)
  10. Folch, J. M. L., and Sloane-Stanley, G. H. : A sample method for the determination of total lipids from animal tissues. J. Biol. Chem., 226, 497 (1957)
  11. SAS Institute : SAS User's Guide : Statistics. SAS Inst, Inc., Cary, NC (1986)
  12. Steel, R. G. D., and Torrie, J. H. : Principles and Procedures of Statistics. McGraw Hill Book Co., NY. (1980)
  13. Duncan. D. B. : Multiple range and multiple F-test Biometrics 11, 1 (1955) https://doi.org/10.2307/3001478
  14. Geffrey, L. Z., Parson, W. W. and Vance, D. E. : Principle of Biochemistry, Wm. C. Brown Communications, 459 (1995)
  15. Qureshi, A. A., Din, Z. Z., Abuirmeileh, N., Burger, W. C., Ahmad, Y. and Elson, C. E. : Suppression of avian hepatic lipid metabolism by solvent extracts of garlic: impact on serum lipids. J. Nutr., 113, 1746 (1983)
  16. Konjufca, V. H., Pesti, G. M. and Bakalli, R. I. : Modulation of cholesterol levels in Broiler Meat by dietary garlic and Copper. Poultry Sci., 76, 1264 (1997) https://doi.org/10.1093/ps/76.9.1264
  17. Kang, J. A. and Kang, J. S. : Effect of garlic and onion on plasma and liver cholesterol and triacylglycerol and platelet aggregation in rats fed basal or cholesterol supplemented diets. Kor. Nutr. Soc., 30, 132 (1997)
  18. Elkin, R. G. and Rogler, J. C. : Effect of lovastatin on laying hen performance and egg choleaterol content. Poultry Sci., 68(Suppl. l):49. (Abstr.) (1989)
  19. Luhman, C. M., Miller, B. G. and Beitz, D. C. : Research Note: The Effect of Feeding Lovastatin and Colestipol on Production and Cholesterol Content of Eggs. Poultry Sci., 69, 852 (1990) https://doi.org/10.3382/ps.0690852
  20. Pesti. G. M., and Bakalli, R. I. : Studies on effect of feeding cupric sulfate pentahydrate to laying hens on egg cholesterol content. Polutry Sci., 77, 1540 (1998) https://doi.org/10.1093/ps/77.10.1540