Nitrogen Removal from Wastewaters by Microalgae Without Consuming Organic Carbon Sources

  • Lee, Kwang-Yong (Institute of Industrial Biotechnology, Department of Biotechnology, Inha University) ;
  • Lee, Choul-Gyun (Institute of Industrial Biotechnology, Department of Biotechnology, Inha University)
  • Published : 2002.12.01

Abstract

The possibility of microalgal nitrogen treatment was tested in wastewaters with a low carbon/nitrogen (C/N) ratio. Chlorella kessleri was cultured in the two different artificial wastewaters with nitrate as a nitrogen source: one contained glucose for an organic carbon source and the other without organic carbon sources. The growth rates of the two cultures were almost identical when the aeration rate was over 1 vvm. These results suggest that microalgae could successfully remove nitrogen from wastewater, as far as the mass transfer of $CO_2$, was not limited. Nitrate was successfully reduced to below 2 mg $NO_3^-$-N/ml from the initial nitrate concentration of 140 mg $NO_3^-$-N/ml in 10 days, even in the wastewater with no organic carbon source. Similar results were obtained when ammonium was used as the sole nitrogen source instead of nitrate. Higher concentrations of nitrogen of 140, 280, 560 and 1,400 mg/ml were also tested and similar amounts of nitrogen were removed by algal cultures without showing any substrate inhibition.

Keywords

References

  1. Wat. Sci. Technol. v.38 Toxicity identification evaluation of ammonia, nitrite and heavy metals at the Stensund Wastewater Aquaculture plant Adamsson,M.;G.Dave;L.Forsberg;B.Guterstam
  2. Appl. Biochem. Biotechnol. v.73 Carbon dioside fixation and polyunsaturated fatty acid production by the red alga Porphyridium cruentum Akimoto,M.;A.Shirai;K.Ohtaguchi;K.Koide https://doi.org/10.1007/BF02785661
  3. Standard Methods for the Examination of Water and Wastewater (20th ed.) APHA,AWWA;WEF
  4. J. Biotechnol. v.70 Commericial production of microalgae: Ponds, tanks, tubes and fermenters Boroxitzka,M.A. https://doi.org/10.1016/S0168-1656(99)00083-8
  5. J. Microbiol. Biotechnol. v.12 Isolation and identification of photosynthetic bacterium useful for wastewater treatment Choi,H.P.;H.J.Kang;H.C.Seo;H.C.Sung
  6. Water Res. v.31 Wastewater nutrient removal by marine microalgae grown on a corrugated raceway Craggs,R.J.;P.J.McAuley;V.J.Smith https://doi.org/10.1016/S0043-1354(96)00093-0
  7. Algal and Cyanobacterial Biotechnology Cresswell,R.C.;T.A.V.Rees;N.Shah
  8. Nature Prod. Rep. v.3 Marine natural products Faulkner,D.J. https://doi.org/10.1039/np9860300001
  9. Energy v.22 Co₂fixation and ethanol production with microalgal photosynthesis and intracellular anaerobic fermentation Hirano,A.;R.Ueda;S.Hirayama;Y.Ogushi https://doi.org/10.1016/S0360-5442(96)00123-5
  10. J. Ferment. Bioeng. v.81 Carbon dioside fixation in batch culture of Chlorella sp. using a photobioreactor with a sunlight-collection device Hirata,S.;M.Hayashitani;M.Taya;S.Tone https://doi.org/10.1016/0922-338X(96)85151-8
  11. J. Microbiol. Biotechnol. v.3 Growth of Spirulina platensis in effluents from wastewater treatment plant of pig farm. Hong,S.S.;N.H.Lee
  12. J. Microbiol. Biotechnol. v.11 Biosorption model for binary adsorption sites Jeon,C.;J.Y.Park;Y.J.Yoo
  13. J. Microbiol. Biotechnol. v.10 Kinetics of removing nitrogenous and phosphorus compounds from swine waste by growth of microalga, Spirulina platensis Kim,M.H.;W.T.Chung;M.K.Lee;J.Y.Lee;S.J.Ohh;J.H.Lee;D.H.Park;D.J.Kim;H.Y.Lee
  14. J. Microbiol. Biotechnol. v.11 Effect of ionic copper toxicity on the growth of green alga, Selenastrum capricornutum Kim,M.K.;R.E.H.Smith
  15. Water Res. v.32 The relative importance of Lemna gibba L. bacteria and algae for the nitrogen and phosphorus removal in duckweed-covered domestic wastewater Korner,S.;J.E.Vermaat https://doi.org/10.1016/S0043-1354(98)00166-3
  16. Resour. Conservation Recycling v.11 Influence of organic-N sources on an algal wastewater treatment system. Lau,P.S.;N.F.Y.Tam;Y.S.Wong https://doi.org/10.1016/0921-3449(94)90090-6
  17. J. Microbiol. Biotechnol. v.10 Effects of NO and SO₂on growth of highly-CO₂-tolerant microalgae Lee,J.H.;J.S.Lee;C.S.Shin;S.C.Park;S.W.Kim
  18. J. Microbiol. Biotechnol. v.11 CO₂fixation by chlorella KR-1 using flue gas and its utilization as a feedstuff for chicks. Lee,J.S.;D.K.Kim;J.P.Lee;S.C.Park;J.H.Koh;S.J.Ohh
  19. Biotechnol. Bioprocess Eng. v.6 Effect of light/dark cycles on wastewater treatments by microalgae Lee,K.Y.;C.G.Lee https://doi.org/10.1007/BF02932550
  20. J. Microbiol. Biotechnol. v.10 Effects of nutrient levels on cell growth and secondary carotenoids formation in the freshwater green alga, Chlorococcum sp. Liu,B.H.;D.H.Zhang;Y.K.Lee
  21. Fuel Energy Abstracts Jan v.25 Sprial tubular bioreactor for hydrogen production by photosynthetic microorganisma: Design and operation Markov,S.A.;P.F.Weaver;M.Seibert
  22. Energy Convers. Mgrnt. v.38 The biological CO₂fixation and utilization project by RITE (2) Murakami,M.;M.Ikenouchi https://doi.org/10.1016/S0196-8904(96)00316-0
  23. J. Ferment. Bioeng. v.86 Improvement of microalgal NO removal in bubble column and airlift reactors Nagase,H.;K.Eguchi;K.Yoshihara;K.Hirata;K.Miyamoto https://doi.org/10.1016/S0922-338X(99)89018-7
  24. Wat. Sci. Tech. v.35 Organic wastewater treatment without greenhouse gas emission by photosynthetic bacteria Nakajima,F.;N.Kamiko;K.Yamamoto https://doi.org/10.1016/S0273-1223(97)00178-9
  25. J. Microbiol. Biotechnol. v.11 Astaxanthin production by Haematococcus pluvialis under various light intensities and wavelengths Park,E.K.;C.G.Lee
  26. Bioscience v.46 Algal diversity and commerical algal products Radmer,R.J. https://doi.org/10.2307/1312833
  27. J. Microbiol. Biotechnol. v.10 Services of algae to the environment Rai,L.C.;H.D.Kumar;F.H.Mohn;C.J.Soeder
  28. Algae Biomass Production and Use Shelef,G.;C.J.Soeder
  29. Energy Convers. Mgmt. v.36 CO₂fixation by artificial weathering of waste concrete and coccolithophorid algae cultures Takano,H.;T.Matsunaga https://doi.org/10.1016/0196-8904(95)00101-I
  30. J. Microbiol. Biotechnol. v.12 Biotechnological potential of marine cyanobacteria in wastewater treatment - Disinfection of raw sewage by Oscillatoria willei BDU 130511 Uma,L.;K.Selvaraj;G.Subramanian;S.Nagarkar;R.Manjula
  31. Handbook of Microalgal Mass Culture Laboratory techniques for the cultivation of microalgae Vonshak,A.;A.Richmond(ed.)