미생물학회지 (Korean Journal of Microbiology)

  • 제38권4호
  • /
  • Pages.235-240
  • /
  • 2002
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  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Protease 생성균 Aeromonas hydrophila PB16의 분리 및 합성폐수처리능

Isolation and Characterization of Aeromons hydrophila PBl6 and Properties of Synthetic Wastewater Degradation

  • 박형수 (삼성엔지니어링 기술연구소) ;
  • 양선영 (삼성엔지니어링 기술연구소) ;
  • 김무훈 (삼성엔지니어링 기술연구소) ;
  • 이종광 (삼성엔지니어링 기술연구소) ;
  • 유용호 (삼성엔지니어링 기술연구소) ;
  • 박두현 (서경대학교 생물공학과)
  • 발행 : 2002.12.01
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초록

식품폐수처리장의 활성슬러지와 논, 밭의 토양에서 우수한 protease 생성 균주을 분리 선별하였다. 이 중 효소활성이 우수한 PB16은 그람 음성, 간균이며 protease activity는 6.49 U/ml이었다. 생리, 생화학적 특성 및 16S rRNA 염기서열분석을 실시한 결과, Aeromonas hydrophila (99.0%) 인 것으로 확인 되었다. Bioscreen C를 사용한 최적 성장조건 평가는 합성폐수에 vitamin과mineral을 첨가한 배지의 중식속도(0.26 $h^{-1}$)가 무 첨가배지(0.21 $h^{-1}$)보다 높았으며, 분리균주의 합성폐수 유기물 제거능 실험에서는 초기 soluble-CODcr 2,472 mg/l인 고농도 합성폐수를 1일, 3일 반응 후 각각 59, 87%의 제거율을 나타내었다.

Protease producing bacterium, PB16 was isolated from food processing wastewater sludge and paddy field soil samples and selected by the clear zone and enzyme activity test. The isolate was gram negative, rod type and its protease productivity was 6.49 U/ml. As a result of API20NE kit test and 16S rDNA sequencying, the isolated PB16 was identified as Aeromonas hydrophila (99%). The growth rate ($h^{-1}$) was 0.21 in synthetic waste water only and 0.26 in synthetic waste water containing vitamin and mineral using a bioscreen C. Synthetic wastewater removal rate was 59 and 87%, respectively after 1 and 3 day reaction (intial CODcr was 2,472 mg/l).

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