Biotechnology and Bioprocess Engineering:BBE

  • 제7권6호
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  • Pages.367-370
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  • 2002
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Hydrolysis of Rice Bran Oil Using Immobilized Lipase in a Stirred-Batch Reactor

  • Murty, V.Ramachandra (Department of Chemical Engineering, Manipal Institute of Technology, A Constituent Institution of Manipal Academy of Higher Education, Deemed University) ;
  • Bhat, Jayadev (Department of Chemical Engineering, Manipal Institute of Technology, A Constituent Institution of Manipal Academy of Higher Education, Deemed University) ;
  • Muniswaran, P.K.A. (Department of Chemical Engineering, Manipal Institute of Technology, A Constituent Institution of Manipal Academy of Higher Education, Deemed University)
  • 발행 : 2002.12.01
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초록

Candida cylindracea lipase was immobilized by adsorption on acid washed glass beads. It was observed that protein loading of the support depends on the size of the particle, with smaller particle containing higher amount of protein per unit weight. Initial reaction rate linearly varied up to enzyme concentration of 17.25 U/mL. Amount of free fatty acids produced was linearly proportional up to the enzyme loading of 1650 $\mu$g/g of bead. Achievement of chemical equilibrium took longer time in the case of less protein loading. Degree of hydrolysis was found to decrease in second and third consecutive batch operations on repeated use of immobilized lipase.

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참고문헌

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