Biotechnology and Bioprocess Engineering:BBE

  • 제7권6호
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  • Pages.331-334
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  • 2002
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Non-invasive Methods for Determination of Cellular Growth in Podophyllum hexandrum Suspension Cultures

  • Chattopadhyay, Saurabh (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology) ;
  • Bisaria, V.S. (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology) ;
  • Scheper, T. (Institut fuer Technische Chemie) ;
  • Srivastava, A.K. (Department of Biochemical Engineering and Biotechnology, Indian Institute of Technology)
  • 발행 : 2002.12.01
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초록

Culture conductivity and on-line NADH fluorescence were used to measure cellular growth in plant cell suspension cultures of Podophyllum hexandrum. An inverse correlation between dry cell weight and medium conductivity was observed during shake flask cultivation. A linear relationship between dry cell weight and culture NADH fluorescence was obtained during the exponential phase of batch cultivation In a bioreactor under the pH stat (pH 6) conditions. It was observed that conductivity measurement were suitable for biomass characterisation under highly dynamic uncontrolled shake flask cultivation conditions. However, if the acid/alkali feeding is done for pH control the conductivity measurement could not be applied. On the other hand the NADH fluorescence measurement allowed online-in situ biomass monitoring of rather heterogenous plant cell suspension cultures in bioreactor even under the most desirable pH stat conditions.

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