Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지

The Effects of 20-hydroxyecdysone in Drosophila Kc Cells on the Ecdysteroidosis

  • Hwang, Seong-Hee (BK21, Science and Technology, Agricultural Biotechnology, Seoul National University) ;
  • Kim, Pan-Gyi (Department of Environmental Health, Yongin University)
  • Published : 2002.11.01
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Abstract

Drosophila Kc cells are ecdysone-responsive : hormone treatment leads rapidly to increased synthesis of several ecdysone-inducible polypeptides (EIPs) and to commitment to eventual proloferative arrest. Later the treated cells undergo morphological transformation, cease to proliferate and to grow. These responses have proven useful as models for studying ecdysone action and environmental endocrine disrupting actions. In this study, we used 20-HE to check out the Kc cells properties to the ecdysone and this properties will be applied to the environmental chemicals to find out the endocrine disrupting action in ecosystem. The cell counts of cultures harvested after 3 days' growth in the presence of 20-hydroxyecdysone. In Kc cell cultures, there were statistically significant different from control cells at 20HE 10/sup 7/-10/sup 5/. The morphological effects of all the hormones were similar, differing only in the dose level at which they were initiated.

Keywords

References

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