Effects of Temperature and Heavy Metals on Extractable Lipofuscin in the Blue Crab, Callinectes sapidus

  • Ju, Se-Jong (Chesapeake Biological Laboratory, University of Maryland Center for Environmental Science) ;
  • Harvey, H.R. (Chesapeake Biological Laboratory, University of Maryland Center for Environmental Science)
  • Published : 2002.12.01

Abstract

The potential role of environmental factors on extractable lipofuscin accumulation rate in the blue crab was studied by examining the effect of temperature on laboratory reared blue crabs and the effect of trace metals from samples collected at impacted sites (Baltimore Harbor) and a relatively pristine site (outdoor ponds at Horn Point Laboratory, Cambridge, MD, USA). Lipofuscin levels did not significantly related with sampling sites or heavy metal concentrations in the crab tissue. Heavy metal body burden was highly variable among sampling sites and tissue types but significantly higher for both impacted areas (Curtis Creek and Inner Harbor) in comparison to the reference site. Among tissue types, gills showed the highest metal concentrations with the exception of Hg, which was highest in muscle tissue. For two groups of crabs that were held at either ambient (4 to 1$0^{\circ}C$) or heated seawater (19$^{\circ}C$) for two months, normalized-lipofuscin levels were significantly different (P-0.001). Results suggest that temperature may affect lipofuscin accumulation (=0.25ng-lipofuscin/mg-protein/temperature-degree day). Therefore, temperature should be considered for accurate age calibration of crab populations using lipofuscin.

Keywords

References

  1. Final report no.UMCES[CBL]97-142 Spatial mapping of sedimentary contaminants in the Baltimore Harbor.Patapsco River/Back River system. Ref Baker, J.;R. Mason;J. Cornwell;J. Ashley;J. Halka;J. Hill
  2. Free Red. Res. v.28 Autoxidation of rat brain homogenate: evidence for spontaneous lipid perosidation. Comparison with the characteristics of $Fe^2+$-and ascorbic acid-stimulated lipid peroxidation Barrier, L.;G. Page;B. Fauconneau;F. Juin
  3. Environ. Sci. Technol. v.33 Speciation and Cycling of Mercury in Lavaca Bay, Texas sediments Bloom N.S.;G.A. Gill;S. Cappellino;C. Dobbs;L. Mcshea;C. Driscoll;R. Mason;J. Rudd
  4. Marine Pollution Heavy metal contamination in the sea Bryan, G.W.
  5. J. Exp. Mar. Biol. Ecol. v.269 The effect of dietary antioxidants on lipofuscin accumulation in the crustacean brain Castro M.;P. Encarnacao;O. Tully
  6. J. Exp. Mar. Biol. Ecol. v.144 The accumulation of fluorescent age pigments in the trochid gastropod Monodonta lineata Clarke A.;M.A. Kendall;D.J. Gore
  7. lkAge Pigments in fungi as a biological marker for heavy metal pollution in aquatic environments Cuomo, V.;E.A. Totaro;F. Vanzanella;P. Russo;S. D'Antonio;M. Caramia
  8. Mar. Ecol. Prog. Ser. v.118 Prey perferences of blue crabs, Callinectes sapidus, feeding on 3 bivalve species Ebersole E.L.;V.S. Kennedy
  9. Mar. Environ. Res. v.35 Crustaceans as models for metal merabolism: I Effects of the molt cycle on blue crab metal metabolism and metallothionein Engel D.W.;M. Brouwer
  10. Mar. Environ. Res. v.49 Trophic accumulation and depuration of mercury by blue crabs(Callinectes sapidus)and pink shrimp(Penaeus duorarum) Evans D.W.;R.D. Kathman;W.W. Walker
  11. Comp. Biochem. Physiol. v.76C Heavy metal detoxification in the mussel Mytilus edulis. Composition of Cd-containing Kidney granules(tertiarylysosomes) George S.G.
  12. Med. Hypoth. v.41 Radiation and aging -free-radical damage, biological response and possible antioxidant intervention Greenstock C.L.
  13. Experientia v.51 Cadmium-induced lipofuscins and effect of zinc on hepatopancreas cells in Idotea baltica Guarino S.M.;F.M. Guarino;G. Tommonaro;M. de Nicola
  14. Advances in the biosciences 64 Osygen radicals, transition metals and aging Gutteridge J.M.C.;Totaro E.A.(ed.);P. Gless(ed.);F.A. Pisanti(ed.)
  15. Biochem. J. v.219 Oxygen toxicity, oxygen radicals, transtion metals and disease Hallwell B.;J.M.C. Gutteridge
  16. Mar. Biol. v.109 Critical aspects of fluorescent age-pigment methodologies: modification for accurate age assessment in aquatic organisms Hill K.T.;C. Wormsley
  17. Mar. Ecol. Prog. Ser. v.122 Age-related cellular and physiological reactions to hypoxia and hyperthermia in marine mussels Hole L.M.;M.N. Moore;D. Bellamy
  18. Mar. Ecol. Prog. Ser. v.185 Use of extractable lipofuscin for age determination of blue crab, Callinectes sapidus Ju S.-J.;D.H. Secor;H.R. Harvey
  19. Mar. Ecol. Prog. Ser. v.224 Growth rate variability and lipofuscin accumulation rates in the blue carb, Callinectes sapidus Ju, S.-J.;D.H. Secor;H.R. Harvey
  20. Mar. Biol. v.14 Some effects of temperature on the growth and metabolic rate of juvenile blue crabs, Callinectes sapidus, in the laboratory Leffler C.W.
  21. Arch. Environ. Contam. Toxicol. v.38 Factors control ling the bioaccumulation of mercury, methymercury, arsenic, selenium, and cadmium by freshwater invertebrates and fish Mason, R.P.;J.-M. Laporte;S. Andres
  22. Mech. Ageing. Dev. v.66 no.2 Heavy metals and lipofuscinogenesis. A study on myocardial cells cultured under varying oxidative stress Marzabadi M.R.;C.B. Jones
  23. Mar. Chem. v.45 A rapid micro-scale method for the extraction and analysis of protein in marine samples Nguyen R.T.;H.R. Harvey
  24. Mar. Biol. v.93 Some limitations on the use of the lipofuscin ageing technique Nicol S.
  25. J. Exp. Mar. Biol. Ecol. v.207 Lipofuscin (age pigment)as an index of crustacean age: correlation with age, temperature and body size in cultured juvenile Homanus Gammarus L O'Donovan V.;O. Tully
  26. Mar. Poll. Bull. v.19 Effect of Heavy metal ions on microorganism aging Pisanti F.A.;L. Lucadamo;F. Vanzanell;E.A. Totro
  27. Bull. Mar. Sci. v.64 Heavy metal bioaccumulation in Puerto Rican blue carbs(Callinectes sapidus) Sastre, M.P.;P. Reyes;H. Ramos;R. Romero;J. Rivera
  28. Comp. Ciochem. Physiol. v.96A Indivudual variation in, and the effect of rearing temperature and body size on, the concentration of fluorescent morphological lipofuscin in the brains of freshwater crayfish, Cherax Cuspidatus(Crustacea: Parastacidae) Sheehy M.R.J.
  29. Mar. Biol. v.121 More accurate chronological age determination of crustaceans from field situations using the physiological age marker, Lipofuscin Sheehy M.R.J.;J.G. Greenwood;D.R. Fielder
  30. J. Exp. Mar. Biol. Ecol. v.209 Predator-prey relationships in mummichogs(Fundulus heteroclitus(L)):Effect of living in a polluted environment Smith G.M.;J.S. Weis
  31. Ph. D. Thesis Models of crustacean growth dynamics Smith S.G.
  32. Musca domestica. Exp. Geront. v.9 Effects of Experimentally altered lifespans on the accumulation of fluorescent age pigments in the housefly Sohal R.S.;H. Donato
  33. Growth Dev. Aging v.63 Brain-tissue accumulation of fluorescent age pigments in four poeciliid fishes (Cyprinodontiformes)and the estimation of "Biological Age" Strauss, R.E
  34. Mar. Environ. Res. v.15 The role of copper level in the formation of neuronal lipofuscin in sprinal ganglia of Topedo marmorata Totaro E.A.;F.A. Pisanti;P. Glees;A. Continllo
  35. Mar. Environ. Res. v.18 The effect of copper pollution on mitochondrial degeneration Totaro, E.A.;F.A. Pisanti;P. Glees;A. Continllo
  36. US EPA, Environmental Criteria Assessment Office v.18 Health effects assessment for lead US EPA
  37. Office of Health and Environmental Assessment Mercury health effects update. Dealth issue assessment. EPA-600/8-74-019F. US EPA US EPA
  38. Office of Health and Environmental Assessment COmpilation of analytical methods. CD-ROM. US EPA US EPA
  39. C.R.C. Crit. Rev. Aquat. Sci. v.1 Heavy metals in marine invertebrates: mechanisms of regulation and toxicity at the cellular level Viarengo A.
  40. Comp. Biochem. Physiol. v.97C Heavy metal effects on liqid peroxidation in the tissues of Mytilus Galloprovincialis Law Viarengo A.;L. Canesi;M. Pettica;G. Poli;M.N. Moore;M. Orunesu
  41. Comp. Biochem. Physiol. v.104C Mechanisms of heavy metal cation homeostasis in marine invertebrates Viarengo A.;J.A. Nott
  42. J. Exp. Zool. v.286 Quantification of the age-pigment lipofusion in brains of knownage, pond-reared prawns Penaeus japonicus (Crustacea, Decapoda) Vila Y.;A. Medina;C. Megina;F. Ramos;I. Sobrino
  43. Bull. Mar. Sci. v.46 Ultrasonic telemerty of small-scale movements and microhabitat selection by molting blue crab(Callinectes sapidus) Wolcott T.G.;A.H. Hines