Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
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Production of Bovine Transgenic Embryos Derived from Non-transfected and Transfected Adult Cells

외부유전자가 도입된 체세포를 이용한 소 형질전환 복제란 생산

  • J. K. Cho (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • M.M.U. Bhuiyan (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • G. Jang (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • Park, E. S. (School of Agriculutral Biotechnology, Seoul National University) ;
  • J. M. Lim (School of Agricultural Biotechnology, Seoul National University) ;
  • S. K. Kang (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • Lee, B. C. (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University) ;
  • W. S. Hwang (Department of Theriogenology & Biotechnology College of Veterinary Medicine, Seoul National University)
  • Published : 2002.08.01
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Abstract

The present study was conducted for the production of transgenic cloned cows those secrete human lactoferricin into milk by somatic cell nuclear transfer (NT). To estimate detrimental effects of gene transfection on transgenic cloned embryo production, development rates of NT embryos were compared between transfected and non-transfected cumulus and ear fibroblast cells. An expression plasmid for human lactofericin (pbeta-LFC) was constructed by inserting a bovine beta-casein promoter, a green fluorescent protein (GFP) marker gene, and human lactoferricin target gene into a pcDNA3 plasmid. Two bovine somatic cell lines (cumulus cell and ear fibroblast) were established and transfected with the expression plasmid using a liposomal transfection reagent, Fugene6 as a carrier. Cumulus cell and ear fibroblast were transfected at the passage of 2 to 4, trypsinized and GFP-expressing cells were randomly selected and used for somatic cell NT. Developmental competences (rates of fusion, cleavage, and blastocyst formation) in bovine transgenic somatic cell NT embryos reconstructed with non-transfectecd cells were significantly higher than those from transfected cells in cumulus cell and ear fibroblast (P＜0.05). This study indicated that transfection of done. cell has detrimental effect on embryo development in bovine transgenic NT.

Keywords

References

  1. Arnold RR, Cole MF and McGhee JR. 1977. A bactericidal effect for human lactoferrin. Science, 197:263-265 https://doi.org/10.1126/science.327545
  2. Baguisi A, Behboodi E, Melican DT, Pollock JS, Destrempes MM, Cammuso C, Williams JL, Nims SD, Porter CA, Midura P, Palacios MJ, Ayres SL, Denniston RS, Hayes ML, Ziomek CA, Meade HM, Godke RA, Gavin WG, Overstrom EW and Echelard Y. 1999. Production of goats by somatic cell nuclear transfer. Nat. Biotechnol., 17:456-461 https://doi.org/10.1038/8632
  3. Bowen RA, Reed ML, Schrieke A, Siedel GE, Jr Stacey A, Thomas SK and Kajikawa O. 1994. Transgenic cattle resulting from biopsied embryos expression of c-ski in a transgenic calf. Biol. Reprod., 50:664-668 https://doi.org/10.1095/biolreprod50.3.664
  4. Chan AWS. 1995. Transgenic animals: Current and alternative strategies. Cloning, 1:25-46 https://doi.org/10.1089/15204559950020076
  5. Cibelli JB, Stice SL, Golueke PJ, Kane JJ, Jerry J, Blackwell C, Ponce de Leon FA and Robl JM. 1998. Cloned transgenic calves produced from non-quiescent fetal fibroblasts. Science, 280:1256-1258 https://doi.org/10.1126/science.280.5367.1256
  6. Ellison RT 3rd and Giehl TJ. 1991. Killing of gram-negative bacteria by lactoferrin and lysozyme. J. Clin. Invest., 88:1080-1091 https://doi.org/10.1172/JCI115407
  7. Gandolfi F, Lavitrano M, Camaioni A, Spadafora G, Siracusa G and Lauria A. 1989. The use of sperm-mediated gene transfer for the generation of transgenic pigs. J. Reprod. Fertil., 4:10
  8. Haskell RE and Bowen RA. 1995. Efficient production of transgenic cattle by retroviral infection of early embryos. Mol. Reprod. Dev., 40:386-390 https://doi.org/10.1002/mrd.1080400316
  9. Hill JR, Burghardt RC, Jones K, Long CR, Looney CR, Shin T, Spencer TE, Thompson JA, Winger QA and Westhusin ME. 2000. Evidence for placental abnormality as the major cause of mortality in first-trimester somatic cell cloned bovine fetuses. Biol. Reprod., 63:1787-1794 https://doi.org/10.1095/biolreprod63.6.1787
  10. Houdebine LM. 2000. Transgenic animal bioreactors. Transgenic Res., 9:305-320 https://doi.org/10.1023/A:1008934912555
  11. Kawamata T, Tooyama I, Yamada T, Walker DG and McGeer PL. 1993. Lactotransferrin immunocytochemistry in Alzheimer and normal human brain. Am. J. Pathol., 142:1574-1585
  12. Klebanoff SJ and Waltersdorph AM. 1990. Peroxidant activity of transferrin and lactoferrin. J. Exp. Med., 172:1293-1303 https://doi.org/10.1084/jem.172.5.1293
  13. Krimpenfort P, Rademakers A, Eyestone W, Van de Schans A, Van der Broek S, Kootwijk E, Platenbury G, Pieger F, Strijker R and De Boer H. 1991. Generation of transgenic dairy cattle using in vitro embryo production. Biotechnology, 9:844-847 https://doi.org/10.1038/nbt0991-844
  14. Kulics J and Kijlstra A. 1987. The effect of lactoferrin on complement mediated modulation of immune complex size. Immunol. Lett., 14:349-353 https://doi.org/10.1016/0165-2478(87)90017-4
  15. Lee BC, Yoon KY, Kim HI, Roh SH and Hwang WS. 1994. Transvaginal ultrasound-guided ovum pick-up in cattle; Effectes of estrus. Korean J. Anim. Sci., 72:434-437 https://doi.org/10.2527/1994.722434x
  16. McCreath KJ, Howcroft J, Campbell KH, Colman A, Schnieke AE and Kind AJ. 2000. Production of gene-targeted sheep by nuclear transfer from cultured somatic cells. Nature, 405:1066-1069 https://doi.org/10.1038/35016604
  17. Petters RM and Sommer JR. 2000. Transgenic animals as models for human disease. Transgenic Res., 9:347-351 https://doi.org/10.1023/A:1008926303533
  18. Reiter B and Perraudin JP. 1998. The antibacterial activity of lactoferrin and neonatal E. coli infections. A selective and critical review. Adv. Exp. Med. Biol., 443:175-188
  19. Rosenkrans CFJ and First NL. 1991. Culture of bovine zygotes to the blastocyst stage: Effect of amino acids and vitamins. Theriogenology, 35:266 https://doi.org/10.1016/0093-691X(91)90242-6
  20. Schellander K, Peli J, Schmall F and Brem G. 1995. Artificial insemination in cattle with DNA-treated sperm. Anim. Biotechnol., 6:41-50 https://doi.org/10.1080/10495399509525831
  21. Schnieke AE, Kind AJ, Ritchie WA, Mycock K, Scott AR, Ritchie M, Wilmut I, Colman A and Campbell KHS. 1997. Human factor IX transgenic sheep produced by transfer of nuclei from transfected fetal fibroblasts. Science, 278:2130-2133 https://doi.org/10.1126/science.278.5346.2130
  22. Stice SL, Strelchenko NS, Keefer CL and Matthews L. 1996. Pluripotent bovine embryonic cell lines direct embryonic development following nuclear transfer. Biol. Reprod., 54:100-110 https://doi.org/10.1095/biolreprod54.1.100
  23. Takahashi Y and First NL. 1992. In vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology, 37:963-978 https://doi.org/10.1016/0093-691X(92)90096-A
  24. Wells DN, Misica PM and Tervit HR. 1999. Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells. Biol. Reprod., 60:996-1005 https://doi.org/10.1095/biolreprod60.4.996
  25. Westhusin ME, Long CR, Shin T, Hill JR, Looney CR, Pryor JH and Piedrahita JA. 2001. Cloning to reproduce desired genotypes. Theriogenology, 55:35-49 https://doi.org/10.1016/S0093-691X(00)00444-1
  26. Wilmut I, Schnieke AE, McWhir J, Kind AJ and Campbell KHS. 1997. Viable offspring derived from fetal and adult mammalian cells. Nature, 385:810-813 https://doi.org/10.1038/385810a0
  27. Yamada Y, Amagasaki T, Jacobsen DW and Green R. 1987. Lactoferrin binding by leukemic cell lines. Blood, 70:264-270
  28. Zakhartchenko V, Mueller S, Alberio R, Schernthaner W, Stojkovic M, Wenigerkind H, Wanke R, Lassnig C, Mueller M, Wolf E and Brem G. 2001. Nuclear transfer in cattle with non-transfected and transfected fetal or cloned transgenic fetal and postnatal fibroblasts. Mol. Reprod. Dev., 60:362-369 https://doi.org/10.1002/mrd.1098